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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
LD50 (estimated) = 127 mg/kg bw in rats
Key value for chemical safety assessment
Acute toxicity: via oral route
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 127 mg/kg bw
- Quality of whole database:
- Value estimated from in vitro toxicity study, with high reliability (R1)
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
- strong cytotoxicity associated with rounded cells and decrease in cell viability (decrease in NRU) were noted at concentrations ≥ 3.17µg/mL,
- the calculated IC50 were 1.502 µg/mL and 1.791 µg/mL in the first and second experiments, respectively,
- these results correspond to estimated LD50 of 123 mg/kg and 131 mg/kg, in the first and second experiments, respectively.
In vitro acute oral toxicity study - 3T3 NRU test (Neutral Red Uptake)
The objective of this study was to evaluate the basal cytotoxicity of the test item, using the 3T3 NRU test (Neutral Red Uptake). The design of the study is based on the OECD Guidance document No. 129 for "Guidance Document on using cytotoxicity test to estimate starting doses for accurate oral systemic toxicity tests". The NRU assay is performed in a dose-response format allowing the calculation of the concentration of the test item that reduces Neutral Red Uptake (NRU) by 50% (IC50). The IC50 value is used in a linear regression equation to estimate the oral LD50 value in rats. The assay evaluates the cytotoxicity of the test item applied to mouse fibroblasts (Balb/c 3T3, clone A 31).
In both experiments, the tested concentrations were: 0.03, 0.1, 0.32, 1, 3.17, 10.01, 31.65 and 100 µg/mL in DMEM0. The following results were obtained after 48 hours incubation:
Under the experimental conditions of this study and after treatment of cells for 48 hours, the test item, is considered as cytotoxic in thisin vitrotest system. The mean IC50 was estimated to be 1.647 µg/mL and the corresponding LD50 for rats was estimated to be 127 mg/kg according to OECD Guidance No. 129.
This result is in line with the LD50 values calculated for many vanadyl derivatives.
Justification for classification or non-classification
According to the CLP Regulation 1272/2008/EC, 3.1.2.1 section, substances can be allocated to one of four toxicity categories based on acute toxicity by the oral, dermal or inhalation route according to the numeric criteria shown in Table 3.1.1:
Oral (mg/kg bodyweight)
Category 1: LD50 ≤ 5
Category 2: 5 <LD50 ≤ 50
Category 3: 50 < LD50 ≤ 300
Category 4: 300 < LD50 ≤ 2 000
The LD50 for the substance under investigation is estimated to be 127 mg/kg body weight in rats, therefore, according to the CLP regulation n. 1272/2008, the substance is classifed as Toxic via Oral Route Category 3.
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