Registration Dossier
Registration Dossier
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EC number: 944-855-6 | CAS number: 199445-85-7
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 March 2017 to 18 April 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study was conducted in accordance with international guidelines and in accordance with GLP. All relevant validity criteria were met.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: stored at approximately 4°C (refrigerator) in the dark under N2 conditions.
- Stability under test conditions: N/A
- Solubility and stability of the test substance in the solvent/vehicle: N/A
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: N/A - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
A mixed population of activated sewage sludge micro-organisms (Test System) was obtained on March 20th, 2017 from the secondary treatment stage of the sewage treatment plant at Villette (STEP Villette, av. de Thônex 105, 1226 THONEX (Geneva, Switzerland)), which treats predominantly domestic sewage.
The sample of activated sewage sludge was maintained on continuous aeration upon receipt. A sample of the activated sewage sludge was washed three times by settlement (centrifuge: Heittich rotenta 460 RS) and suspension in culture medium. To remove any excessive amounts of Dissolved Organic Carbon (DOC) that may have been present, the solution was stirred and maintained on with pure oxygen at room temperature. Determination of dry weight is made to inoculate final solution with 30mg/L dry weight activated sludge. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 50 mg/L
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The culture medium used in this study (see Appendix 1) was that recommended in the OECD Guideline N° 301F.
Solution A
(KH2PO4) 8.50 g/L
(K2HPO4) 21.75g/L
(Na2HPO4,2H2O) 33.40 g/L
(NH4Cl) 0.50 g/L
Solution B
(CaCl2,2H2O) 36.40 g/L
Solution C
(MgSO4,7H2O) 22.50 g/L
Solution D
(FeCl3,6H2O) 0.25 g/L
For 1 l of test medium, mix 10 ml of solution a) with 800 ml water and then add 1 ml each of solutions b), c) and d) and make up to 1 000 ml with water
- Additional substrate: N/A
- Solubilising agent (type and concentration if used): N/A
- Test temperature: 22°C +/- 1°C.
- pH: On day 28, all vessels were sampled for pH.
- pH adjusted: no
- CEC (meq/100 g): N/A
- Aeration of dilution water: N/A
- Suspended solids concentration: 30mg/L dry weight (activated sludge)
- Continuous darkness: no (diffuse light)
- Other:
TEST SYSTEM
- Culturing apparatus: The system consists of a sample flask sealed by a sensor head/CO2 trap in a temperature controlled incubator. The samples were stirred for the duration of the study with a magnetically coupled stirrer.
- Number of culture flasks/concentration: Blanks x 2, Test Item x 3, Reference x 1, Toxicity Control x 1, Abiotic Control x 1
- Method used to create aerobic conditions: Oxygen
- Method used to create anaerobic conditions: N/A
- Measuring equipment: WTW oxitopC
- Test performed in closed vessels due to significant volatility of test substance: No (sealed system as per OECD 301F)
- Test performed in open system: No
- Details of trap for CO2 and volatile organics if used: NaOH
- Other:
SAMPLING
- Sampling frequency: Daily
- Sampling method: WTW oxitopC calculate automatically the consumption of oxygen.
- Sterility check if applicable: N/A
- Sample storage before analysis: N/A
- Other: N/A
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control:
- Other: N/A - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 59
- St. dev.:
- 2.08
- Sampling time:
- 28 d
- Details on results:
- The test item (test 1) attained 58% degradation after 28 days.
The test item (test 2) attained 61% degradation after 28 days and didn’t satisfy the 10-day window validation criterion.
The test item (test 3) attained 57% degradation after 28 days. - Results with reference substance:
- Sodium Benzoate attained 81% degradation after 14 days thereby confirming the suitability of the inoculum and test conditions.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item (test 1 and 3) attained respectively 58% and 57% degradation after 28 days. Under the conditions of the test, the test item cannot be considered to be readily biodegradable.
The test item (test 2) attained respectively 61% and didn’t satisfy the 10-day window validation criterion. Under the conditions of the test, the test item cannot be considered to be readily biodegradable. - Executive summary:
The study was performed to assess the ready biodegradability of the test item in an aerobic aqueous media. The method followed that described in the OECD Guideline for the testing of Chemicals (1992) N°301 F, “Ready Biodegradability; Manometric Respirometry Test”.
In addition, the procedures were designed to meet the test method of the Council Regulation (EC) No. 440/2008 of 30 May 2008, Publication No. L142, Part C.4-D laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH).
The test item, at a concentration of 50.05mg/L in test 1, at a concentration of 50.55mg/L in test 2 and at a concentration of 50.00mg/L in test 3 was exposed to activated sewage sludge micro-organisms with culture medium in sealed culture vessels in diffused light at 22°C ± 1°C for 28 days.
The degradation of the test item was assessed by the measurement of daily oxygen consumption on days 0 and 28. Control solutions with inoculum and the reference material, sodium benzoate, together with a toxicity control were used for validation purposes.
The test item (test 1 and 3) attained respectively58% and57% degradation after 28 days. Under the conditions of the test, the test item cannot be considered to be readily biodegradable.
The test item (test 2) attained respectively61% anddidn’t satisfy the 10-day window validation criterion. Under the conditions of the test, the test item cannot be considered to be readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 March 2017 to 01 June 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study was conducted in accordance with international guidelines and in accordance with GLP. All relevant validity criterua were met.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- A mixed population of activated sewage sludge micro-organisms (Test System) was obtained on May 3rd, 2017 from the secondary treatment stage of the sewage treatment plant at Villette (STEP Villette, av. de Thônex 105, 1226 THONEX (Geneva, Switzerland)), which treats predominantly domestic sewage.
The sample of activated sewage sludge was maintained on continuous aeration upon receipt. A sample of the activated sewage sludge was washed three times by settlement (centrifuge: Heittich rotenta 460 RS) and suspension in culture medium. To remove any excessive amounts of Dissolved Organic Carbon (DOC) that may have been present, the solution was stirred and maintained on with pure oxygen at room temperature. Determination of dry weight is made to inoculate final solution with 30mg/L dry weight activated sludge. - Duration of test (contact time):
- 28 d
- Initial conc.:
- >= 44.29 - <= 44.67 mg/L
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The culture medium used in this study (see Appendix 1) was that recommended in the OECD Guideline N° 301F.
Solution A
(KH2PO4) 8.50 g/L
(K2HPO4) 21.75g/L
(Na2HPO4,2H2O) 33.40 g/L
(NH4Cl) 0.50 g/L
Solution B
(CaCl2,2H2O) 36.40 g/L
Solution C
(MgSO4,7H2O) 22.50 g/L
Solution D
(FeCl3,6H2O) 0.25 g/L
For 1 L of test medium, mix 10 mL of solution a) with 800 mL water and then add 1 ml each of solutions b), c) and d) and make up to 1 000 mL with water
- Additional substrate: N/A
- Solubilising agent (type and concentration if used): N/A
- Test temperature: 22 °C +/- 1 °C.
- pH: On day 28, all vessels were sampled for pH.
- pH adjusted: no
- CEC (meq/100 g): N/A
- Aeration of dilution water: N/A
- Suspended solids concentration: 30mg/L dry weight (activated sludge)
- Continuous darkness: no (diffuse light)
- Other:
TEST SYSTEM
- Culturing apparatus: The system consists of a sample flask sealed by a sensor head/CO2 trap in a temperature controlled incubator. The samples were stirred for the duration of the study with a magnetically coupled stirrer.
- Number of culture flasks/concentration: Blanks x 2, Test Item x 3, Reference x 1, Toxicity Control x 1, Abiotic Control x 1
- Method used to create aerobic conditions: Oxygen
- Method used to create anaerobic conditions: N/A
- Measuring equipment: WTW oxitopC
- Test performed in closed vessels due to significant volatility of test substance: No (sealed system as per OECD 301F)
- Test performed in open system: No
- Details of trap for CO2 and volatile organics if used: NaOH
- Other:
SAMPLING
- Sampling frequency: Daily
- Sampling method: WTW oxitopC calculate automatically the consumption of oxygen.
- Sterility check if applicable: N/A
- Sample storage before analysis: N/A
- Other: N/A
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control:
- Other: N/A - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 67
- St. dev.:
- 5.2
- Sampling time:
- 28 d
- Details on results:
- The test item (test 1) attained 64% degradation after 28 days and satisfied the 10-day window validation criterion.
The test item (test 2) attained 64% degradation after 28 days and satisfied the 10-day window validation criterion.
The test item (test 3) attained 73% degradation after 28 days and satisfied the 10-day window validation criterion.
The toxicity test attained 57% degradation after 14 days thereby confirming that the test item was not toxic to the sewage treatmentn micro-organismsused in the study. - Results with reference substance:
- Sodium Benzoate attained 86% degradation after 14 days thereby confirming the suitability of the inoculum and test conditions.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item (replicates 1, 2 and 3) attained respectively 64%, 64% and 73% degradation after 28 days and satisfied the 10-day window. Under the conditions of the test, the test item can be considered to be readily biodegradable.
- Executive summary:
The study was performed to assess the ready biodegradability of the test item in an aerobic aqueous media. The method followed that described in the OECD Guideline for the testing of Chemicals (1992) N°301 F, “Ready Biodegradability; Manometric Respirometry Test”. In addition, the procedures were designed to meet the test method of the European Council Regulation (EC) No. 440/2008 of 30 May 2008, Publication No. L142, Part C.4-D laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH).
The test item, at a concentration of 44.60mg/L in test 1, at a concentration of 44.29mg/L in test 2 and at a concentration of 44.67mg/L in test 3 was exposed to activated sewage sludge micro-organisms with culture medium in sealed culture vessels in diffused light at 22°C ± 1°C for 28 days.
The degradation of the test item was assessed by the measurement of daily oxygen consumption on days 0 and 28. Control solutions with inoculum and the reference material, sodium benzoate, together with a toxicity control were used for validation purposes.
The test item (replicates 1, 2 and 3) attained respectively 64%, 64% and 73% degradation after 28 days and satisfied the 10-day window validation criterion. Under the conditions of the test, the test item can be considered to be readily biodegradable.
Referenceopen allclose all
The toxicity test attained 54% degradation after 14 days thereby confirming that the test item was not toxic to the sewage treatmentmicro-organismsused in the study.
Preparation of Test Solutions
|
flask |
Test Item |
reference |
activated sludge 1.5g/L |
medium |
vol total |
pH final |
|
|
|
mg |
mg |
L |
L |
L |
||
blank 1 |
1 |
xxxxxxxx |
xxxxxxxx |
0.0080 |
0.3920 |
0.4000 |
7.29 |
|
blank 2 |
2 |
xxxxxxxx |
xxxxxxxx |
0.0080 |
0.3920 |
0.4000 |
7.29 |
|
test 1 |
3 |
20.02 |
xxxxxxxx |
0.0080 |
0.3920 |
0.4000 |
7.32 |
|
test 2 |
4 |
20.22 |
xxxxxxxx |
0.0080 |
0.3920 |
0.4000 |
7.33 |
|
test 3 |
5 |
20 |
xxxxxxxx |
0.0080 |
0.3920 |
0.4000 |
7.34 |
|
reference |
6 |
xxxxxxxx |
20.08 |
0.0080 |
0.3920 |
0.4000 |
7.47 |
|
toxicity |
7 |
19.91 |
20.2 |
0.0080 |
0.3920 |
0.4000 |
7.48 |
|
*abiotic |
8 |
19.96 |
xxxxxxxx |
0.0080 |
0.3920 |
0.4000 |
7.4 |
* NaN3 50 g/L
Cumulative Biochemical Oxygen Demand (mgO2/L) in test flask during the test period of 28 days
days |
min |
bl1 |
bl2 |
test1 |
test2 |
test3 |
reference |
toxicity |
abiotic |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
1456 |
2.5 |
4.9 |
4.9 |
2.5 |
2.5 |
9.9 |
22.2 |
2.5 |
2 |
2912 |
7.4 |
7.4 |
7.4 |
4.9 |
4.9 |
49.4 |
59.3 |
2.5 |
3 |
4368 |
9.9 |
9.9 |
7.4 |
7.4 |
7.4 |
61.8 |
64.2 |
2.5 |
4 |
5712 |
12.4 |
12.4 |
9.9 |
7.4 |
7.4 |
69.2 |
69.2 |
2.5 |
5 |
7168 |
12.4 |
14.8 |
9.9 |
9.9 |
9.9 |
71.7 |
71.7 |
2.5 |
6 |
8624 |
14.8 |
14.8 |
9.9 |
9.9 |
9.9 |
76.6 |
74.1 |
2.5 |
7 |
10080 |
14.8 |
17.3 |
12.4 |
9.9 |
9.9 |
79.1 |
76.6 |
2.5 |
8 |
11536 |
17.3 |
17.3 |
12.4 |
9.9 |
12.4 |
81.5 |
79.1 |
2.5 |
9 |
12992 |
17.3 |
19.8 |
12.4 |
12.4 |
9.9 |
84 |
86.5 |
2.5 |
10 |
14448 |
19.8 |
19.8 |
12.4 |
12.4 |
12.4 |
86.5 |
93.9 |
2.5 |
11 |
15792 |
19.8 |
22.2 |
14.8 |
12.4 |
12.4 |
89 |
98.8 |
2.5 |
12 |
17248 |
22.2 |
22.2 |
14.8 |
14.8 |
12.4 |
91.4 |
104 |
2.5 |
13 |
18704 |
22.2 |
22.2 |
14.8 |
14.8 |
14.8 |
91.4 |
119 |
4.9 |
14 |
20160 |
22.2 |
24.7 |
22.2 |
17.3 |
17.3 |
91.4 |
146 |
4.9 |
15 |
21616 |
22.2 |
24.7 |
32.1 |
19.8 |
24.7 |
93.9 |
161 |
4.9 |
16 |
23072 |
24.7 |
24.7 |
42 |
39.5 |
37.1 |
93.9 |
178 |
4.9 |
17 |
24528 |
24.7 |
24.7 |
54.4 |
64.2 |
54.4 |
96.4 |
185 |
4.9 |
18 |
25872 |
24.7 |
27.2 |
71.7 |
79.1 |
69.2 |
96.4 |
190 |
4.9 |
19 |
27328 |
24.7 |
27.2 |
84 |
91.4 |
81.5 |
96.4 |
195 |
4.9 |
20 |
28784 |
24.7 |
27.2 |
93.9 |
96.4 |
91.4 |
96.4 |
198 |
2.5 |
21 |
30240 |
24.7 |
27.2 |
98.8 |
101 |
93.9 |
96.4 |
200 |
2.5 |
22 |
31696 |
24.7 |
27.2 |
101 |
104 |
96.4 |
96.4 |
203 |
4.9 |
23 |
33152 |
27.2 |
27.2 |
106 |
106 |
98.8 |
98.8 |
205 |
4.9 |
24 |
34608 |
27.2 |
27.2 |
106 |
109 |
104 |
98.8 |
205 |
4.9 |
25 |
35952 |
27.2 |
29.7 |
109 |
111 |
104 |
98.8 |
208 |
2.5 |
26 |
37408 |
27.2 |
29.7 |
111 |
114 |
106 |
98.8 |
208 |
2.5 |
27 |
38864 |
27.2 |
29.7 |
111 |
116 |
109 |
98.8 |
210 |
2.5 |
28 |
40320 |
27.2 |
27.2 |
111 |
116 |
109 |
98.8 |
210 |
2.5 |
Percentage Biodegradation in Test 1
days |
blank 1 |
blank 2 |
average |
test1 |
BOD (mgO2) |
BOD(mgO2/mg test) |
% degradation |
|
NH3 |
||||||||
0 |
0 |
0 |
0 |
0 |
0.0 |
0.00 |
0 |
|
1 |
2.5 |
4.9 |
3.7 |
4.9 |
0.5 |
0.02 |
1 |
|
2 |
7.4 |
7.4 |
7.4 |
7.4 |
0.0 |
0.00 |
0 |
|
3 |
9.9 |
9.9 |
9.9 |
7.4 |
-1.0 |
-0.05 |
-2 |
|
4 |
12.4 |
12.4 |
12.4 |
9.9 |
-1.0 |
-0.05 |
-2 |
|
5 |
12.4 |
14.8 |
13.6 |
9.9 |
-1.5 |
-0.07 |
-3 |
|
6 |
14.8 |
14.8 |
14.8 |
9.9 |
-2.0 |
-0.10 |
-3 |
|
7 |
14.8 |
17.3 |
16.05 |
12.4 |
-1.5 |
-0.07 |
-3 |
|
8 |
17.3 |
17.3 |
17.3 |
12.4 |
-2.0 |
-0.10 |
-3 |
|
9 |
17.3 |
19.8 |
18.55 |
12.4 |
-2.5 |
-0.12 |
-4 |
|
10 |
19.8 |
19.8 |
19.8 |
12.4 |
-3.0 |
-0.15 |
-5 |
|
11 |
19.8 |
22.2 |
21 |
14.8 |
-2.5 |
-0.12 |
-4 |
|
12 |
22.2 |
22.2 |
22.2 |
14.8 |
-3.0 |
-0.15 |
-5 |
|
13 |
22.2 |
22.2 |
22.2 |
14.8 |
-3.0 |
-0.15 |
-5 |
|
14 |
22.2 |
24.7 |
23.45 |
22.2 |
-0.5 |
-0.02 |
-1 |
|
15 |
22.2 |
24.7 |
23.45 |
32.1 |
3.5 |
0.17 |
6 |
|
16 |
24.7 |
24.7 |
24.7 |
42 |
6.9 |
0.35 |
12 |
|
17 |
24.7 |
24.7 |
24.7 |
54.4 |
11.9 |
0.59 |
21 |
|
18 |
24.7 |
27.2 |
25.95 |
71.7 |
18.3 |
0.91 |
32 |
|
19 |
24.7 |
27.2 |
25.95 |
84 |
23.2 |
1.16 |
40 |
|
20 |
24.7 |
27.2 |
25.95 |
93.9 |
27.2 |
1.36 |
47 |
|
21 |
24.7 |
27.2 |
25.95 |
98.8 |
29.1 |
1.46 |
50 |
|
22 |
24.7 |
27.2 |
25.95 |
101 |
30.0 |
1.50 |
52 |
|
23 |
27.2 |
27.2 |
27.2 |
106 |
31.5 |
1.57 |
55 |
|
24 |
27.2 |
27.2 |
27.2 |
106 |
31.5 |
1.57 |
55 |
|
25 |
27.2 |
29.7 |
28.45 |
109 |
32.2 |
1.61 |
56 |
|
26 |
27.2 |
29.7 |
28.45 |
111 |
33.0 |
1.65 |
57 |
|
27 |
27.2 |
29.7 |
28.45 |
111 |
33.0 |
1.65 |
57 |
|
28 |
27.2 |
27.2 |
27.2 |
111 |
33.5 |
1.67 |
58 |
Percentage Biodegradation in Test 2
days |
blank 1 |
blank 2 |
average |
test2 |
BOD (mgO2) |
BOD(mgO2/mg test) |
% degradation |
|
NH3 |
||||||||
0 |
0 |
0 |
0 |
0 |
0.0 |
0.00 |
0 |
|
1 |
2.5 |
4.9 |
3.7 |
2.5 |
-0.5 |
-0.02 |
-1 |
|
2 |
7.4 |
7.4 |
7.4 |
4.9 |
-1.0 |
-0.05 |
-2 |
|
3 |
9.9 |
9.9 |
9.9 |
7.4 |
-1.0 |
-0.05 |
-2 |
|
4 |
12.4 |
12.4 |
12.4 |
7.4 |
-2.0 |
-0.10 |
-3 |
|
5 |
12.4 |
14.8 |
13.6 |
9.9 |
-1.5 |
-0.07 |
-3 |
|
6 |
14.8 |
14.8 |
14.8 |
9.9 |
-2.0 |
-0.10 |
-3 |
|
7 |
14.8 |
17.3 |
16.05 |
9.9 |
-2.5 |
-0.12 |
-4 |
|
8 |
17.3 |
17.3 |
17.3 |
9.9 |
-3.0 |
-0.15 |
-5 |
|
9 |
17.3 |
19.8 |
18.55 |
12.4 |
-2.5 |
-0.12 |
-4 |
|
10 |
19.8 |
19.8 |
19.8 |
12.4 |
-3.0 |
-0.15 |
-5 |
|
11 |
19.8 |
22.2 |
21 |
12.4 |
-3.4 |
-0.17 |
-6 |
|
12 |
22.2 |
22.2 |
22.2 |
14.8 |
-3.0 |
-0.15 |
-5 |
|
13 |
22.2 |
22.2 |
22.2 |
14.8 |
-3.0 |
-0.15 |
-5 |
|
14 |
22.2 |
24.7 |
23.45 |
17.3 |
-2.5 |
-0.12 |
-4 |
|
15 |
22.2 |
24.7 |
23.45 |
19.8 |
-1.5 |
-0.07 |
-3 |
|
16 |
24.7 |
24.7 |
24.7 |
39.5 |
5.9 |
0.29 |
10 |
|
17 |
24.7 |
24.7 |
24.7 |
64.2 |
15.8 |
0.78 |
27 |
|
18 |
24.7 |
27.2 |
25.95 |
79.1 |
21.3 |
1.05 |
36 |
|
19 |
24.7 |
27.2 |
25.95 |
91.4 |
26.2 |
1.29 |
45 |
|
20 |
24.7 |
27.2 |
25.95 |
96.4 |
28.2 |
1.39 |
48 |
|
21 |
24.7 |
27.2 |
25.95 |
101 |
30.0 |
1.48 |
51 |
|
22 |
24.7 |
27.2 |
25.95 |
104 |
31.2 |
1.54 |
53 |
|
23 |
27.2 |
27.2 |
27.2 |
106 |
31.5 |
1.56 |
54 |
|
24 |
27.2 |
27.2 |
27.2 |
109 |
32.7 |
1.62 |
56 |
|
25 |
27.2 |
29.7 |
28.45 |
111 |
33.0 |
1.63 |
57 |
|
26 |
27.2 |
29.7 |
28.45 |
114 |
34.2 |
1.69 |
59 |
|
27 |
27.2 |
29.7 |
28.45 |
116 |
35.0 |
1.73 |
60 |
|
28 |
27.2 |
27.2 |
27.2 |
116 |
35.5 |
1.76 |
61 |
Percentage Biodegradation in Test 3
days |
blank 1 |
blank 2 |
average |
test3 |
BOD (mgO2) |
BOD(mgO2/mg test) |
% degradation |
|
NH3 |
||||||||
0 |
0 |
0 |
0 |
0 |
0.0 |
0.00 |
0 |
|
1 |
2.5 |
4.9 |
3.7 |
2.5 |
-0.5 |
-0.02 |
-1 |
|
2 |
7.4 |
7.4 |
7.4 |
4.9 |
-1.0 |
-0.05 |
-2 |
|
3 |
9.9 |
9.9 |
9.9 |
7.4 |
-1.0 |
-0.05 |
-2 |
|
4 |
12.4 |
12.4 |
12.4 |
7.4 |
-2.0 |
-0.10 |
-3 |
|
5 |
12.4 |
14.8 |
13.6 |
9.9 |
-1.5 |
-0.07 |
-3 |
|
6 |
14.8 |
14.8 |
14.8 |
9.9 |
-2.0 |
-0.10 |
-3 |
|
7 |
14.8 |
17.3 |
16.05 |
9.9 |
-2.5 |
-0.12 |
-4 |
|
8 |
17.3 |
17.3 |
17.3 |
12.4 |
-2.0 |
-0.10 |
-3 |
|
9 |
17.3 |
19.8 |
18.55 |
9.9 |
-3.5 |
-0.17 |
-6 |
|
10 |
19.8 |
19.8 |
19.8 |
12.4 |
-3.0 |
-0.15 |
-5 |
|
11 |
19.8 |
22.2 |
21 |
12.4 |
-3.4 |
-0.17 |
-6 |
|
12 |
22.2 |
22.2 |
22.2 |
12.4 |
-3.9 |
-0.20 |
-7 |
|
13 |
22.2 |
22.2 |
22.2 |
14.8 |
-3.0 |
-0.15 |
-5 |
|
14 |
22.2 |
24.7 |
23.45 |
17.3 |
-2.5 |
-0.12 |
-4 |
|
15 |
22.2 |
24.7 |
23.45 |
24.7 |
0.5 |
0.03 |
1 |
|
16 |
24.7 |
24.7 |
24.7 |
37.1 |
5.0 |
0.25 |
9 |
|
17 |
24.7 |
24.7 |
24.7 |
54.4 |
11.9 |
0.59 |
21 |
|
18 |
24.7 |
27.2 |
25.95 |
69.2 |
17.3 |
0.87 |
30 |
|
19 |
24.7 |
27.2 |
25.95 |
81.5 |
22.2 |
1.11 |
38 |
|
20 |
24.7 |
27.2 |
25.95 |
91.4 |
26.2 |
1.31 |
45 |
|
21 |
24.7 |
27.2 |
25.95 |
93.9 |
27.2 |
1.36 |
47 |
|
22 |
24.7 |
27.2 |
25.95 |
96.4 |
28.2 |
1.41 |
49 |
|
23 |
27.2 |
27.2 |
27.2 |
98.8 |
28.6 |
1.43 |
50 |
|
24 |
27.2 |
27.2 |
27.2 |
104 |
30.7 |
1.54 |
53 |
|
25 |
27.2 |
29.7 |
28.45 |
104 |
30.2 |
1.51 |
52 |
|
26 |
27.2 |
29.7 |
28.45 |
106 |
31.0 |
1.55 |
54 |
|
27 |
27.2 |
29.7 |
28.45 |
109 |
32.2 |
1.61 |
56 |
|
28 |
27.2 |
27.2 |
27.2 |
109 |
32.7 |
1.64 |
57 |
Percentage Biodegradation in Reference
days |
blank 1 |
blank 2 |
average |
ref |
BOD (mgO2) |
BOD(mgO2/mg test) |
% degradation |
|
NH3 |
||||||||
0 |
0 |
0 |
0 |
0 |
0.0 |
0.00 |
0 |
|
1 |
2.5 |
4.9 |
3.7 |
9.9 |
2.5 |
0.12 |
7 |
|
2 |
7.4 |
7.4 |
7.4 |
49.4 |
16.8 |
0.84 |
50 |
|
3 |
9.9 |
9.9 |
9.9 |
61.8 |
20.8 |
1.03 |
62 |
|
4 |
12.4 |
12.4 |
12.4 |
69.2 |
22.7 |
1.13 |
68 |
|
5 |
12.4 |
14.8 |
13.6 |
71.7 |
23.2 |
1.16 |
69 |
|
6 |
14.8 |
14.8 |
14.8 |
76.6 |
24.7 |
1.23 |
74 |
|
7 |
14.8 |
17.3 |
16.05 |
79.1 |
25.2 |
1.26 |
75 |
|
8 |
17.3 |
17.3 |
17.3 |
81.5 |
25.7 |
1.28 |
77 |
|
9 |
17.3 |
19.8 |
18.55 |
84 |
26.2 |
1.30 |
78 |
|
10 |
19.8 |
19.8 |
19.8 |
86.5 |
26.7 |
1.33 |
80 |
|
11 |
19.8 |
22.2 |
21 |
89 |
27.2 |
1.35 |
81 |
|
12 |
22.2 |
22.2 |
22.2 |
91.4 |
27.7 |
1.38 |
83 |
|
13 |
22.2 |
22.2 |
22.2 |
91.4 |
27.7 |
1.38 |
83 |
|
14 |
22.2 |
24.7 |
23.45 |
91.4 |
27.2 |
1.35 |
81 |
|
15 |
22.2 |
24.7 |
23.45 |
93.9 |
28.2 |
1.40 |
84 |
|
16 |
24.7 |
24.7 |
24.7 |
93.9 |
27.7 |
1.38 |
83 |
|
17 |
24.7 |
24.7 |
24.7 |
96.4 |
28.7 |
1.43 |
86 |
|
18 |
24.7 |
27.2 |
25.95 |
96.4 |
28.2 |
1.40 |
84 |
|
19 |
24.7 |
27.2 |
25.95 |
96.4 |
28.2 |
1.40 |
84 |
|
20 |
24.7 |
27.2 |
25.95 |
96.4 |
28.2 |
1.40 |
84 |
|
21 |
24.7 |
27.2 |
25.95 |
96.4 |
28.2 |
1.40 |
84 |
|
22 |
24.7 |
27.2 |
25.95 |
96.4 |
28.2 |
1.40 |
84 |
|
23 |
27.2 |
27.2 |
27.2 |
98.8 |
28.6 |
1.43 |
86 |
|
24 |
27.2 |
27.2 |
27.2 |
98.8 |
28.6 |
1.43 |
86 |
|
25 |
27.2 |
29.7 |
28.45 |
98.8 |
28.1 |
1.40 |
84 |
|
26 |
27.2 |
29.7 |
28.45 |
98.8 |
28.1 |
1.40 |
84 |
|
27 |
27.2 |
29.7 |
28.45 |
98.8 |
28.1 |
1.40 |
84 |
|
28 |
27.2 |
27.2 |
27.2 |
98.8 |
28.6 |
1.43 |
86 |
Percentage Biodegradation in Toxicity
days |
blanc 1 |
blanc 2 |
moy |
toxicite |
BOD (mgO2) |
BOD(mgO2/mg test) |
% degradation |
|
NH3 |
||||||||
0 |
0 |
0 |
0 |
0 |
0.0 |
0.00 |
0 |
|
1 |
2.5 |
4.9 |
3.7 |
22.2 |
7.4 |
0.37 |
8 |
|
2 |
7.4 |
7.4 |
7.4 |
59.3 |
20.8 |
1.03 |
23 |
|
3 |
9.9 |
9.9 |
9.9 |
64.2 |
21.7 |
1.08 |
24 |
|
4 |
12.4 |
12.4 |
12.4 |
69.2 |
22.7 |
1.12 |
25 |
|
5 |
12.4 |
14.8 |
13.6 |
71.7 |
23.2 |
1.15 |
26 |
|
6 |
14.8 |
14.8 |
14.8 |
74.1 |
23.7 |
1.17 |
26 |
|
7 |
14.8 |
17.3 |
16.05 |
76.6 |
24.2 |
1.20 |
27 |
|
8 |
17.3 |
17.3 |
17.3 |
79.1 |
24.7 |
1.22 |
27 |
|
9 |
17.3 |
19.8 |
18.55 |
86.5 |
27.2 |
1.35 |
30 |
|
10 |
19.8 |
19.8 |
19.8 |
93.9 |
29.6 |
1.47 |
33 |
|
11 |
19.8 |
22.2 |
21 |
98.8 |
31.1 |
1.54 |
34 |
|
12 |
22.2 |
22.2 |
22.2 |
104 |
32.7 |
1.62 |
36 |
|
13 |
22.2 |
22.2 |
22.2 |
119 |
38.7 |
1.92 |
42 |
|
14 |
22.2 |
24.7 |
23.45 |
146 |
49.0 |
2.43 |
54 |
|
15 |
22.2 |
24.7 |
23.45 |
161 |
55.0 |
2.72 |
60 |
|
16 |
24.7 |
24.7 |
24.7 |
178 |
61.3 |
3.04 |
67 |
|
17 |
24.7 |
24.7 |
24.7 |
185 |
64.1 |
3.17 |
70 |
|
18 |
24.7 |
27.2 |
25.95 |
190 |
65.6 |
3.25 |
72 |
|
19 |
24.7 |
27.2 |
25.95 |
195 |
67.6 |
3.35 |
74 |
|
20 |
24.7 |
27.2 |
25.95 |
198 |
68.8 |
3.41 |
76 |
|
21 |
24.7 |
27.2 |
25.95 |
200 |
69.6 |
3.45 |
76 |
|
22 |
24.7 |
27.2 |
25.95 |
203 |
70.8 |
3.51 |
78 |
|
23 |
27.2 |
27.2 |
27.2 |
205 |
71.1 |
3.52 |
78 |
|
24 |
27.2 |
27.2 |
27.2 |
205 |
71.1 |
3.52 |
78 |
|
25 |
27.2 |
29.7 |
28.45 |
208 |
71.8 |
3.56 |
79 |
|
26 |
27.2 |
29.7 |
28.45 |
208 |
71.8 |
3.56 |
79 |
|
27 |
27.2 |
29.7 |
28.45 |
210 |
72.6 |
3.60 |
80 |
|
28 |
27.2 |
27.2 |
27.2 |
210 |
73.1 |
3.62 |
80 |
Percentage Biodegradation in Abiotic Test
|
blanc 1 |
blanc 2 |
moy |
abiotic |
BOD (mgO2) |
BOD(mgO2/mg test) |
% degradation |
|
NH3 |
||||||||
0 |
0 |
0 |
0 |
0 |
0.0 |
0.00 |
0 |
|
1 |
2.5 |
4.9 |
3.7 |
2.5 |
-0.5 |
-0.02 |
-1 |
|
2 |
7.4 |
7.4 |
7.4 |
2.5 |
-2.0 |
-0.10 |
-3 |
|
3 |
9.9 |
9.9 |
9.9 |
2.5 |
-3.0 |
-0.15 |
-5 |
|
4 |
12.4 |
12.4 |
12.4 |
2.5 |
-4.0 |
-0.20 |
-7 |
|
5 |
12.4 |
14.8 |
13.6 |
2.5 |
-4.4 |
-0.22 |
-8 |
|
6 |
14.8 |
14.8 |
14.8 |
2.5 |
-4.9 |
-0.25 |
-9 |
|
7 |
14.8 |
17.3 |
16.05 |
2.5 |
-5.4 |
-0.27 |
-9 |
|
8 |
17.3 |
17.3 |
17.3 |
2.5 |
-5.9 |
-0.30 |
-10 |
|
9 |
17.3 |
19.8 |
18.55 |
2.5 |
-6.4 |
-0.32 |
-11 |
|
10 |
19.8 |
19.8 |
19.8 |
2.5 |
-6.9 |
-0.35 |
-12 |
|
11 |
19.8 |
22.2 |
21 |
2.5 |
-7.4 |
-0.37 |
-13 |
|
12 |
22.2 |
22.2 |
22.2 |
2.5 |
-7.9 |
-0.39 |
-14 |
|
13 |
22.2 |
22.2 |
22.2 |
4.9 |
-6.9 |
-0.35 |
-12 |
|
14 |
22.2 |
24.7 |
23.45 |
4.9 |
-7.4 |
-0.37 |
-13 |
|
15 |
22.2 |
24.7 |
23.45 |
4.9 |
-7.4 |
-0.37 |
-13 |
|
16 |
24.7 |
24.7 |
24.7 |
4.9 |
-7.9 |
-0.40 |
-14 |
|
17 |
24.7 |
24.7 |
24.7 |
4.9 |
-7.9 |
-0.40 |
-14 |
|
18 |
24.7 |
27.2 |
25.95 |
4.9 |
-8.4 |
-0.42 |
-15 |
|
19 |
24.7 |
27.2 |
25.95 |
4.9 |
-8.4 |
-0.42 |
-15 |
|
20 |
24.7 |
27.2 |
25.95 |
2.5 |
-9.4 |
-0.47 |
-16 |
|
21 |
24.7 |
27.2 |
25.95 |
2.5 |
-9.4 |
-0.47 |
-16 |
|
22 |
24.7 |
27.2 |
25.95 |
4.9 |
-8.4 |
-0.42 |
-15 |
|
23 |
27.2 |
27.2 |
27.2 |
4.9 |
-8.9 |
-0.45 |
-15 |
|
24 |
27.2 |
27.2 |
27.2 |
4.9 |
-8.9 |
-0.45 |
-15 |
|
25 |
27.2 |
29.7 |
28.45 |
2.5 |
-10.4 |
-0.52 |
-18 |
|
26 |
27.2 |
29.7 |
28.45 |
2.5 |
-10.4 |
-0.52 |
-18 |
|
27 |
27.2 |
29.7 |
28.45 |
2.5 |
-10.4 |
-0.52 |
-18 |
|
28 |
27.2 |
27.2 |
27.2 |
2.5 |
-9.9 |
-0.49 |
-17 |
Cumulative Biochemical Oxygen Demand (mgO2/L) in test flask during the test period of 28 days
| days | min | bl1 | bl2 | test1 | test2 | test3 | reference | toxicity | abiotic |
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | 1456 | 4.1 | 6.2 | 4.1 | 4.1 | 4.1 | 10.3 | 18.5 | 0 |
| 2 | 2912 | 10.3 | 10.3 | 6.2 | 6.2 | 6.2 | 47.3 | 51.4 | 0 |
| 3 | 4368 | 12.3 | 12.3 | 8.2 | 8.2 | 8.2 | 57.6 | 55.5 | 0 |
| 4 | 5712 | 14.4 | 14.4 | 10.3 | 10.3 | 8.2 | 63.7 | 59.6 | 0 |
| 5 | 7168 | 16.4 | 16.4 | 10.3 | 10.3 | 8.2 | 69.9 | 63.7 | 0 |
| 6 | 8624 | 16.4 | 18.5 | 12.3 | 10.3 | 10.3 | 74 | 65.8 | 0 |
| 7 | 10080 | 18.5 | 18.5 | 12.3 | 12.3 | 10.3 | 76.1 | 69.9 | 0 |
| 8 | 11536 | 18.5 | 20.6 | 12.3 | 12.3 | 12.3 | 78.1 | 74 | 0 |
| 9 | 12992 | 20.6 | 20.6 | 14.4 | 12.3 | 12.3 | 80.2 | 80.2 | 0 |
| 10 | 14448 | 20.6 | 20.6 | 14.4 | 14.4 | 14.4 | 82.2 | 88.4 | 0 |
| 11 | 15792 | 20.6 | 20.6 | 14.4 | 14.4 | 14.4 | 84.3 | 96.6 | 0 |
| 12 | 17248 | 22.6 | 22.6 | 16.4 | 16.4 | 16.4 | 84.3 | 105 | 0 |
| 13 | 18704 | 22.6 | 22.6 | 20.6 | 20.6 | 20.6 | 84.3 | 119 | 0 |
| 14 | 20160 | 22.6 | 22.6 | 30.8 | 26.7 | 30.8 | 86.4 | 138 | 0 |
| 15 | 21616 | 24.7 | 22.6 | 53.5 | 39.1 | 55.5 | 86.4 | 152 | 0 |
| 16 | 23072 | 24.7 | 22.6 | 80.2 | 63.7 | 80.2 | 88.4 | 164 | 0 |
| 17 | 24528 | 24.7 | 22.6 | 86.4 | 80.2 | 88.4 | 88.4 | 171 | 0 |
| 18 | 25872 | 24.7 | 24.7 | 90.5 | 86.4 | 92.5 | 88.4 | 173 | 0 |
| 19 | 27328 | 24.7 | 24.7 | 94.6 | 92.5 | 98.7 | 90.5 | 177 | 0 |
| 20 | 28784 | 24.7 | 24.7 | 98.7 | 96.6 | 101 | 90.5 | 179 | 0 |
| 21 | 30240 | 24.7 | 24.7 | 103 | 101 | 103 | 90.5 | 183 | 0 |
| 22 | 31696 | 24.7 | 24.7 | 103 | 103 | 107 | 92.5 | 185 | 0 |
| 23 | 33152 | 26.7 | 24.7 | 105 | 105 | 109 | 92.5 | 185 | 0 |
| 24 | 34608 | 26.7 | 24.7 | 107 | 105 | 111 | 92.5 | 187 | 0 |
| 25 | 35952 | 26.7 | 26.7 | 107 | 105 | 115 | 92.5 | 189 | 0 |
| 26 | 37408 | 26.7 | 26.7 | 107 | 107 | 115 | 92.5 | 189 | 0 |
| 27 | 38864 | 26.7 | 24.7 | 109 | 107 | 119 | 94.6 | 191 | 0 |
| 28 | 40320 | 26.7 | 26.7 | 109 | 109 | 121 | 94.6 | 193 | 0 |
Percentage Biodegradation in Test 1
| days | blank 1 | blank 2 | average | test1 | BOD (mgO2) | BOD(mgO2/mg test) | % degradation |
| NH3 | |||||||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | 4.1 | 6.2 | 5.15 | 4.1 | -0.5 | -0.02 | -1 |
| 2 | 10.3 | 10.3 | 10.3 | 6.2 | -1.8 | -0.09 | -3 |
| 3 | 12.3 | 12.3 | 12.3 | 8.2 | -1.8 | -0.09 | -3 |
| 4 | 14.4 | 14.4 | 14.4 | 10.3 | -1.8 | -0.09 | -3 |
| 5 | 16.4 | 16.4 | 16.4 | 10.3 | -2.7 | -0.14 | -5 |
| 6 | 16.4 | 18.5 | 17.45 | 12.3 | -2.3 | -0.12 | -4 |
| 7 | 18.5 | 18.5 | 18.5 | 12.3 | -2.8 | -0.14 | -5 |
| 8 | 18.5 | 20.6 | 19.55 | 12.3 | -3.3 | -0.16 | -6 |
| 9 | 20.6 | 20.6 | 20.6 | 14.4 | -2.8 | -0.14 | -5 |
| 10 | 20.6 | 20.6 | 20.6 | 14.4 | -2.8 | -0.14 | -5 |
| 11 | 20.6 | 20.6 | 20.6 | 14.4 | -2.8 | -0.14 | -5 |
| 12 | 22.6 | 22.6 | 22.6 | 16.4 | -2.8 | -0.14 | -5 |
| 13 | 22.6 | 22.6 | 22.6 | 20.6 | -0.9 | -0.04 | -2 |
| 14 | 22.6 | 22.6 | 22.6 | 30.8 | 3.7 | 0.18 | 6 |
| 15 | 24.7 | 22.6 | 23.65 | 53.5 | 13.4 | 0.67 | 23 |
| 16 | 24.7 | 22.6 | 23.65 | 80.2 | 25.4 | 1.27 | 44 |
| 17 | 24.7 | 22.6 | 23.65 | 86.4 | 28.2 | 1.41 | 49 |
| 18 | 24.7 | 24.7 | 24.7 | 90.5 | 29.6 | 1.48 | 51 |
| 19 | 24.7 | 24.7 | 24.7 | 94.6 | 31.5 | 1.57 | 54 |
| 20 | 24.7 | 24.7 | 24.7 | 98.7 | 33.3 | 1.66 | 57 |
| 21 | 24.7 | 24.7 | 24.7 | 103 | 35.2 | 1.76 | 61 |
| 22 | 24.7 | 24.7 | 24.7 | 103 | 35.2 | 1.76 | 61 |
| 23 | 26.7 | 24.7 | 25.7 | 105 | 35.7 | 1.78 | 62 |
| 24 | 26.7 | 24.7 | 25.7 | 107 | 36.6 | 1.82 | 63 |
| 25 | 26.7 | 26.7 | 26.7 | 107 | 36.1 | 1.8 | 62 |
| 26 | 26.7 | 26.7 | 26.7 | 107 | 36.1 | 1.8 | 62 |
| 27 | 26.7 | 24.7 | 25.7 | 109 | 37.5 | 1.87 | 65 |
| 28 | 26.7 | 26.7 | 26.7 | 109 | 37 | 1.85 | 64 |
Percentage Biodegradation in Test 2
| days | blank 1 | blank 2 | average | test2 | BOD (mgO2) | BOD(mgO2/mg test) | % degradation |
| NH3 | |||||||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | 4.1 | 6.2 | 5.15 | 4.1 | -0.5 | -0.02 | -1 |
| 2 | 10.3 | 10.3 | 10.3 | 6.2 | -1.8 | -0.09 | -3 |
| 3 | 12.3 | 12.3 | 12.3 | 8.2 | -1.8 | -0.09 | -3 |
| 4 | 14.4 | 14.4 | 14.4 | 10.3 | -1.8 | -0.09 | -3 |
| 5 | 16.4 | 16.4 | 16.4 | 10.3 | -2.7 | -0.14 | -5 |
| 6 | 16.4 | 18.5 | 17.45 | 10.3 | -3.2 | -0.16 | -6 |
| 7 | 18.5 | 18.5 | 18.5 | 12.3 | -2.8 | -0.14 | -5 |
| 8 | 18.5 | 20.6 | 19.55 | 12.3 | -3.3 | -0.16 | -6 |
| 9 | 20.6 | 20.6 | 20.6 | 12.3 | -3.7 | -0.19 | -6 |
| 10 | 20.6 | 20.6 | 20.6 | 14.4 | -2.8 | -0.14 | -5 |
| 11 | 20.6 | 20.6 | 20.6 | 14.4 | -2.8 | -0.14 | -5 |
| 12 | 22.6 | 22.6 | 22.6 | 16.4 | -2.8 | -0.14 | -5 |
| 13 | 22.6 | 22.6 | 22.6 | 20.6 | -0.9 | -0.05 | -2 |
| 14 | 22.6 | 22.6 | 22.6 | 26.7 | 1.8 | 0.09 | 3 |
| 15 | 24.7 | 22.6 | 23.65 | 39.1 | 7 | 0.35 | 12 |
| 16 | 24.7 | 22.6 | 23.65 | 63.7 | 18 | 0.9 | 31 |
| 17 | 24.7 | 22.6 | 23.65 | 80.2 | 25.4 | 1.28 | 44 |
| 18 | 24.7 | 24.7 | 24.7 | 86.4 | 27.8 | 1.39 | 48 |
| 19 | 24.7 | 24.7 | 24.7 | 92.5 | 30.5 | 1.53 | 53 |
| 20 | 24.7 | 24.7 | 24.7 | 96.6 | 32.4 | 1.62 | 56 |
| 21 | 24.7 | 24.7 | 24.7 | 101 | 34.3 | 1.72 | 60 |
| 22 | 24.7 | 24.7 | 24.7 | 103 | 35.2 | 1.77 | 61 |
| 23 | 26.7 | 24.7 | 25.7 | 105 | 35.7 | 1.79 | 62 |
| 24 | 26.7 | 24.7 | 25.7 | 105 | 35.7 | 1.79 | 62 |
| 25 | 26.7 | 26.7 | 26.7 | 105 | 35.2 | 1.77 | 61 |
| 26 | 26.7 | 26.7 | 26.7 | 107 | 36.1 | 1.81 | 63 |
| 27 | 26.7 | 24.7 | 25.7 | 107 | 36.6 | 1.84 | 64 |
| 28 | 26.7 | 26.7 | 26.7 | 109 | 37 | 1.86 | 64 |
Percentage Biodegradation in Test 3
| days | blank 1 | blank 2 | average | test3 | BOD (mgO2) | BOD(mgO2/mg test) | % degradation |
| NH3 | |||||||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | 4.1 | 6.2 | 5.15 | 4.1 | -0.5 | -0.02 | -1 |
| 2 | 10.3 | 10.3 | 10.3 | 6.2 | -1.8 | -0.09 | -3 |
| 3 | 12.3 | 12.3 | 12.3 | 8.2 | -1.8 | -0.09 | -3 |
| 4 | 14.4 | 14.4 | 14.4 | 8.2 | -2.8 | -0.14 | -5 |
| 5 | 16.4 | 16.4 | 16.4 | 8.2 | -3.7 | -0.18 | -6 |
| 6 | 16.4 | 18.5 | 17.45 | 10.3 | -3.2 | -0.16 | -6 |
| 7 | 18.5 | 18.5 | 18.5 | 10.3 | -3.7 | -0.18 | -6 |
| 8 | 18.5 | 20.6 | 19.55 | 12.3 | -3.3 | -0.16 | -6 |
| 9 | 20.6 | 20.6 | 20.6 | 12.3 | -3.7 | -0.19 | -6 |
| 10 | 20.6 | 20.6 | 20.6 | 14.4 | -2.8 | -0.14 | -5 |
| 11 | 20.6 | 20.6 | 20.6 | 14.4 | -2.8 | -0.14 | -5 |
| 12 | 22.6 | 22.6 | 22.6 | 16.4 | -2.8 | -0.14 | -5 |
| 13 | 22.6 | 22.6 | 22.6 | 20.6 | -0.9 | -0.04 | -2 |
| 14 | 22.6 | 22.6 | 22.6 | 30.8 | 3.7 | 0.18 | 6 |
| 15 | 24.7 | 22.6 | 23.65 | 55.5 | 14.3 | 0.71 | 25 |
| 16 | 24.7 | 22.6 | 23.65 | 80.2 | 25.4 | 1.27 | 44 |
| 17 | 24.7 | 22.6 | 23.65 | 88.4 | 29.1 | 1.45 | 50 |
| 18 | 24.7 | 24.7 | 24.7 | 92.5 | 30.5 | 1.52 | 53 |
| 19 | 24.7 | 24.7 | 24.7 | 98.7 | 33.3 | 1.66 | 57 |
| 20 | 24.7 | 24.7 | 24.7 | 101 | 34.3 | 1.71 | 59 |
| 21 | 24.7 | 24.7 | 24.7 | 103 | 35.2 | 1.75 | 61 |
| 22 | 24.7 | 24.7 | 24.7 | 107 | 37 | 1.84 | 64 |
| 23 | 26.7 | 24.7 | 25.7 | 109 | 37.5 | 1.86 | 65 |
| 24 | 26.7 | 24.7 | 25.7 | 111 | 38.4 | 1.91 | 66 |
| 25 | 26.7 | 26.7 | 26.7 | 115 | 39.7 | 1.98 | 68 |
| 26 | 26.7 | 26.7 | 26.7 | 115 | 39.7 | 1.98 | 68 |
| 27 | 26.7 | 24.7 | 25.7 | 119 | 42 | 2.09 | 72 |
| 28 | 26.7 | 26.7 | 26.7 | 121 | 42.4 | 2.11 | 73 |
Percentage Biodegradation in Reference
| days | blank 1 | blank 2 | average | ref | BOD (mgO2) | BOD(mgO2/mg test) | % degradation |
| NH3 | |||||||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | 4.1 | 6.2 | 5.15 | 10.3 | 2.3 | 0.12 | 7 |
| 2 | 10.3 | 10.3 | 10.3 | 47.3 | 16.7 | 0.83 | 50 |
| 3 | 12.3 | 12.3 | 12.3 | 57.6 | 20.4 | 1.01 | 61 |
| 4 | 14.4 | 14.4 | 14.4 | 63.7 | 22.2 | 1.1 | 66 |
| 5 | 16.4 | 16.4 | 16.4 | 69.9 | 24.1 | 1.2 | 72 |
| 6 | 16.4 | 18.5 | 17.45 | 74 | 25.4 | 1.27 | 76 |
| 7 | 18.5 | 18.5 | 18.5 | 76.1 | 25.9 | 1.29 | 77 |
| 8 | 18.5 | 20.6 | 19.55 | 78.1 | 26.3 | 1.31 | 79 |
| 9 | 20.6 | 20.6 | 20.6 | 80.2 | 26.8 | 1.33 | 80 |
| 10 | 20.6 | 20.6 | 20.6 | 82.2 | 27.7 | 1.38 | 83 |
| 11 | 20.6 | 20.6 | 20.6 | 84.3 | 28.7 | 1.43 | 86 |
| 12 | 22.6 | 22.6 | 22.6 | 84.3 | 27.8 | 1.38 | 83 |
| 13 | 22.6 | 22.6 | 22.6 | 84.3 | 27.8 | 1.38 | 83 |
| 14 | 22.6 | 22.6 | 22.6 | 86.4 | 28.7 | 1.43 | 86 |
| 15 | 24.7 | 22.6 | 23.65 | 86.4 | 28.2 | 1.4 | 84 |
| 16 | 24.7 | 22.6 | 23.65 | 88.4 | 29.1 | 1.45 | 87 |
| 17 | 24.7 | 22.6 | 23.65 | 88.4 | 29.1 | 1.45 | 87 |
| 18 | 24.7 | 24.7 | 24.7 | 88.4 | 28.7 | 1.43 | 86 |
| 19 | 24.7 | 24.7 | 24.7 | 90.5 | 29.6 | 1.47 | 88 |
| 20 | 24.7 | 24.7 | 24.7 | 90.5 | 29.6 | 1.47 | 88 |
| 21 | 24.7 | 24.7 | 24.7 | 90.5 | 29.6 | 1.47 | 88 |
| 22 | 24.7 | 24.7 | 24.7 | 92.5 | 30.5 | 1.52 | 91 |
| 23 | 26.7 | 24.7 | 25.7 | 92.5 | 30.1 | 1.5 | 90 |
| 24 | 26.7 | 24.7 | 25.7 | 92.5 | 30.1 | 1.5 | 90 |
| 25 | 26.7 | 26.7 | 26.7 | 92.5 | 29.6 | 1.47 | 88 |
| 26 | 26.7 | 26.7 | 26.7 | 92.5 | 29.6 | 1.47 | 88 |
| 27 | 26.7 | 24.7 | 25.7 | 94.6 | 31 | 1.54 | 93 |
| 28 | 26.7 | 26.7 | 26.7 | 94.6 | 30.6 | 1.52 | 91 |
Percentage Biodegradation in Toxicity Control
| days | blank 1 | blank 2 | average | toxicity | BOD (mgO2) | BOD(mgO2/mg test) | % degradation |
| NH3 | |||||||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | 4.1 | 6.2 | 5.15 | 18.5 | 6 | 0.3 | 7 |
| 2 | 10.3 | 10.3 | 10.3 | 51.4 | 18.5 | 0.93 | 20 |
| 3 | 12.3 | 12.3 | 12.3 | 55.5 | 19.4 | 0.97 | 21 |
| 4 | 14.4 | 14.4 | 14.4 | 59.6 | 20.3 | 1.02 | 22 |
| 5 | 16.4 | 16.4 | 16.4 | 63.7 | 21.3 | 1.06 | 23 |
| 6 | 16.4 | 18.5 | 17.45 | 65.8 | 21.8 | 1.09 | 24 |
| 7 | 18.5 | 18.5 | 18.5 | 69.9 | 23.1 | 1.16 | 25 |
| 8 | 18.5 | 20.6 | 19.55 | 74 | 24.5 | 1.23 | 27 |
| 9 | 20.6 | 20.6 | 20.6 | 80.2 | 26.8 | 1.34 | 30 |
| 10 | 20.6 | 20.6 | 20.6 | 88.4 | 30.5 | 1.53 | 34 |
| 11 | 20.6 | 20.6 | 20.6 | 96.6 | 34.2 | 1.71 | 38 |
| 12 | 22.6 | 22.6 | 22.6 | 105 | 37.1 | 1.85 | 41 |
| 13 | 22.6 | 22.6 | 22.6 | 119 | 43.4 | 2.17 | 48 |
| 14 | 22.6 | 22.6 | 22.6 | 138 | 51.9 | 2.6 | 57 |
| 15 | 24.7 | 22.6 | 23.65 | 152 | 57.8 | 2.89 | 64 |
| 16 | 24.7 | 22.6 | 23.65 | 164 | 63.2 | 3.16 | 70 |
| 17 | 24.7 | 22.6 | 23.65 | 171 | 66.3 | 3.32 | 73 |
| 18 | 24.7 | 24.7 | 24.7 | 173 | 66.7 | 3.34 | 74 |
| 19 | 24.7 | 24.7 | 24.7 | 177 | 68.5 | 3.43 | 76 |
| 20 | 24.7 | 24.7 | 24.7 | 179 | 69.4 | 3.47 | 76 |
| 21 | 24.7 | 24.7 | 24.7 | 183 | 71.2 | 3.56 | 78 |
| 22 | 24.7 | 24.7 | 24.7 | 185 | 72.1 | 3.61 | 79 |
| 23 | 26.7 | 24.7 | 25.7 | 185 | 71.7 | 3.59 | 79 |
| 24 | 26.7 | 24.7 | 25.7 | 187 | 72.6 | 3.63 | 80 |
| 25 | 26.7 | 26.7 | 26.7 | 189 | 73 | 3.65 | 80 |
| 26 | 26.7 | 26.7 | 26.7 | 189 | 73 | 3.65 | 80 |
| 27 | 26.7 | 24.7 | 25.7 | 191 | 74.4 | 3.72 | 82 |
| 28 | 26.7 | 26.7 | 26.7 | 193 | 74.8 | 3.74 | 82 |
Percentage Biodegradation in Abiotic Test
| days | blank 1 | blank 2 | average | abiotic | BOD (mgO2) | BOD(mgO2/mg test) | % degradation |
| NH3 | |||||||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | 4.1 | 6.2 | 5.15 | 0 | -2.3 | -0.11 | -4 |
| 2 | 10.3 | 10.3 | 10.3 | 0 | -4.6 | -0.23 | -8 |
| 3 | 12.3 | 12.3 | 12.3 | 0 | -5.5 | -0.27 | -10 |
| 4 | 14.4 | 14.4 | 14.4 | 0 | -6.5 | -0.32 | -11 |
| 5 | 16.4 | 16.4 | 16.4 | 0 | -7.4 | -0.37 | -13 |
| 6 | 16.4 | 18.5 | 17.45 | 0 | -7.9 | -0.39 | -13 |
| 7 | 18.5 | 18.5 | 18.5 | 0 | -8.3 | -0.41 | -14 |
| 8 | 18.5 | 20.6 | 19.55 | 0 | -8.8 | -0.44 | -15 |
| 9 | 20.6 | 20.6 | 20.6 | 0 | -9.3 | -0.46 | -16 |
| 10 | 20.6 | 20.6 | 20.6 | 0 | -9.3 | -0.46 | -16 |
| 11 | 20.6 | 20.6 | 20.6 | 0 | -9.3 | -0.46 | -16 |
| 12 | 22.6 | 22.6 | 22.6 | 0 | -10.2 | -0.5 | -17 |
| 13 | 22.6 | 22.6 | 22.6 | 0 | -10.2 | -0.5 | -17 |
| 14 | 22.6 | 22.6 | 22.6 | 0 | -10.2 | -0.5 | -17 |
| 15 | 24.7 | 22.6 | 23.65 | 0 | -10.6 | -0.53 | -18 |
| 16 | 24.7 | 22.6 | 23.65 | 0 | -10.6 | -0.53 | -18 |
| 17 | 24.7 | 22.6 | 23.65 | 0 | -10.6 | -0.53 | -18 |
| 18 | 24.7 | 24.7 | 24.7 | 0 | -11.1 | -0.55 | -19 |
| 19 | 24.7 | 24.7 | 24.7 | 0 | -11.1 | -0.55 | -19 |
| 20 | 24.7 | 24.7 | 24.7 | 0 | -11.1 | -0.55 | -19 |
| 21 | 24.7 | 24.7 | 24.7 | 0 | -11.1 | -0.55 | -19 |
| 22 | 24.7 | 24.7 | 24.7 | 0 | -11.1 | -0.55 | -19 |
| 23 | 26.7 | 24.7 | 25.7 | 0 | -11.6 | -0.57 | -20 |
| 24 | 26.7 | 24.7 | 25.7 | 0 | -11.6 | -0.57 | -20 |
| 25 | 26.7 | 26.7 | 26.7 | 0 | -12 | -0.6 | -21 |
| 26 | 26.7 | 26.7 | 26.7 | 0 | -12 | -0.6 | -21 |
| 27 | 26.7 | 24.7 | 25.7 | 0 | -11.6 | -0.57 | -20 |
| 28 | 26.7 | 26.7 | 26.7 | 0 | -12 | -0.6 | -21 |
Description of key information
Biodegradation (28 -days): 67 % (meets 10 -day window criterion), readily biodegradable, OECD 301F, Anon, 2017b
Biodegradation (28 -days): 59 %, not readily biodegradable, OECD 301F, Anon., 2017a
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Two key studies were submitted for the biodegradation in water: screening test (Anon., 2017a and Anon., 2017b). Both studies were considered reliable with a reliability score of 1.
Anon., 2017a was performed to assess the ready biodegradability of the test item in an aerobic aqueous media. The method followed that described in the OECD Guideline for the testing of Chemicals (1992) N°301 F, “Ready Biodegradability; Manometric Respirometry Test”. In addition, the procedures were designed to meet the test methods of the European Council Regulation (EC) No. 440/2008 of 30 May 2008, Publication No. L142, Part C.4-D laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH). The test item - at a concentration of 50.05 mg/L in test 1, at a concentration of 50.55 mg/L in test 2 and at a concentration of 50.00 mg/L in test 3 - was exposed to activated sewage sludge micro-organisms with culture medium in sealed culture vessels in diffuse light at 22 °C ± 1 °C for 28 days. The degradation of the test item was assessed by the measurement of daily oxygen consumption on days 0 and 28. Control solutions with inoculum and the reference material, sodium benzoate, together with a toxicity control were used for validation purposes. The test item (replicates 1 and 3) attained respectively 58% and 57% degradation after 28 days. Under the conditions of the test, the test item cannot be considered to be readily biodegradable.
The test item (replicate 2) attained 61% degradation and didn’t satisfy the 10-day window validation criterion. Under the conditions of this test, the test item cannot be considered to be readily biodegradable.
Anon., 2017b was performed to assess the ready biodegradability of the test item in an aerobic aqueous media. The method followed that described in the OECD Guideline for the testing of Chemicals (1992) N°301 F, “Ready Biodegradability; Manometric Respirometry Test”. In addition, the procedures were designed to meet the test methods of the European Council Regulation (EC) No. 440/2008 of 30 May 2008, Publication No. L142, Part C.4-D laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH). The test item, at a concentration of 44.60 mg/L in test 1, at a concentration of 44.29 mg/L in test 2 and at a concentration of 44.67 mg/L in test 3 was exposed to activated sewage sludge micro-organisms with culture medium in sealed culture vessels in diffuse light at 22 °C ± 1 °C for 28 days. The degradation of the test item was assessed by the measurement of daily oxygen consumption on days 0 and 28. Control solutions with inoculum and the reference material, sodium benzoate, together with a toxicity control were used for validation purposes. The test item (replicates 1, 2 and 3) attained 64%, 64% and 73% degradation, respectively, after 28 days and satisfied the 10-day window validation criterion. Under the conditions of the test, the test item can be considered to be readily biodegradable.
In accordance with ECHA Guidance on Information Requirements and Chemical Safety Assessment, Chapter R.7B (Version 4.0 - June 2017), it is recognised that ready biodegradability tests may sometimes fail to report a representative result because of the stringent test conditions, positive results (i.e. those showing ready biodegradabality) should supersede negative results. The only significant difference in the test system between the two studies was the addition rate (50 mg/L Anon., 2017a and 45 mg/L Anon., 2017b). The source of inoculum was also identical for both studies, indicating that this was not a factor affecting the two studies. Given that the only significant difference in test systems was based on addition rates of test item, both of which were within guideline specification, the positive result from Anon., 2017b is considered the most relevant for concluding on this endpoint.
The substance is considered readily biodegradable.
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