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EC number: 943-438-6 | CAS number: 90063-59-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 July 1990 - 24 August 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Study performed according to OECD Guideline 301B in compliance with GLP. However, the results of this key study are compiled in a summary report only representing the most crucial results, lacking information on the blanks and reference substance.
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Principles of method if other than guideline:
- The study was conducted in accordance to draft Ecotoxicology SOP 158 01. However, the ferric chloride stock solution contained 0.25g not 0.2 g and the EDTA stock solution contained 0.4g (as recommended in the 1988 OECD Ring Test) and not 0.5g as advised by the draft SOP 158 01.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- Measurement of CO2/DIC
- Details on sampling:
- - Sampling frequency: Day 4, 7, 11, 14, 18, 21, 25 and 28
- Sampling method: At each interval a vessel is removed and the carbon dioxide concentration of the headspace gas determined. The seal of the vessel is then broken and the concentration of inorganic carbon in the test medium measured. - Vehicle:
- no
- Details on test solutions:
- APPLICATION OF TEST SOLUTION
The test substance was added directly to the appropriate vessels with a high quality liquid delivery syringe. The volume added( 1-2 μl) is negligible compared to the total volume of 100 m in each vessel and is not taken into consideration when determining the results. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source of inoculum/activated sludge: Secondary effluent from an unacclimatised activated sludge plant at URL North
- Preparation of inoculum for exposure: 10% by volume of activated sludge plant secondary effluent was filtered through Whatman filter paper (541) to remove coarse particulate matter.
- Pretreatment: The level of dissolved inorganic carbon (DIC) was reduced by sparging the filtered effluent with nitrogen after prior adjustment of the pH to 6.5. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 28 d
- Test temperature:
- Air temperature 18-21°C
Temperature in the liquid medium 21.5-23°C - pH:
- 6.5
- Nominal and measured concentrations:
- 10 mg/L organic carbon corresponding to 14.2 mg/L test substance (nominal)
- Details on test conditions:
- TEST SYSTEM
- Culturing apparatus: 160mL vessels (hypovials) containing 100mL mineral salts medium inoculated with secondary effluent and the respective test of reference substance
- Method used to create aerobic conditions: headspace of vessels filled with air (60mL), containing approx. 6 times the mass of oxygen required for the complete oxidation of the substance.
- Test performed in closed vessels due to significant volatility of test substance: yes
TEST CONDITIONS
- Composition of medium: mineral salts medium (as recommended in the 1988 OECD Ring test)
- Test temperature: Air temperature 18-21°C; Temperature in the liquid medium 21.5-23°C
- pH: 6.5
- pH adjusted: yes
- Other: EDTA stock solution (for the preparation of the mineral salts medium) contained 0.4g instead of 0.5g as recommended in the draft Ecotoxicology SOP 158 01; Nominal Carbon added to medium: 940 µg (assuming 100% purity of compound) - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 14.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- Biodegradation of the test substance during 28d
- Details on results:
- Substance was found to be readily biodegradable hence no toxic or other inhibitory effect on microorganisms is concluded.
- Results with reference substance (positive control):
- The test does not mention inclusion of a toxicity control.
- Validity criteria fulfilled:
- not specified
- Remarks:
- no information on difference between replicates and CO2 evolution in blank, toxicity control not applicable as substance was found to be readily biodegradable.
- Conclusions:
- Based on the results of a ready biodegradability screening test (OECD TG301B), the substance is not considered to be toxic or inhibit micro-organisms when dosed 14.2 mg/L Litsea cubeba.
- Executive summary:
The toxicity of Litsea cubeba to microorganisms can be inferred from a study conducted in accordance to GLP and to an equivilant guideline with OECDTG 301B (carbon dioxide evolution test -modified Sturm test, closed vessel). In this study, activated sludge was exposed to 14.2 mg/L test substance for 28 days. Analysis for CO2/DIC in the liquid medium was analyzed on day 4, 7, 11, 14, 18, 21, 25 and 28.
Litsea cubeba was biodegraded by 71.2 % at day 14 and completely biodegraded at day 28. However as there was complete biodegradation, the test substance is not considered to inhibit mcirobial activity. It can therefore be concluded that Litsea cubeba is non-toxic to microorganisms at the nominal tested concentration of 14.2 mg/L which is therefore considered the NOEC.
Reference
Percentage Biodegradation of the test substance Litsea cubeba:
Day No. |
% Biodegradation (a) |
R488 (b) |
|
4 |
52.2 |
7 |
71.2 |
11 |
82.4 |
14 |
89.7 |
18 |
89.2 |
21 |
93.2 |
25 |
100.7 |
28 |
103.9 |
95% confidence interval |
102.6 – 105.3 |
(a) Based on nominal carbon concentration which assumes 100% purity of the named test substance unless specified otherwise
(b) Actual percentage biodegradation
Description of key information
The toxicity of Litsea cubeba to microorganisms can be inferred from a study conducted in accordance to GLP and to an equivilant guideline with OECDTG 301B (carbon dioxide evolution test -modified Sturm test, closed vessel). In this study, activated sludge was exposed to 14.2 mg/L test substance for 28 days. Analysis for CO2/DIC in the liquid medium was analyzed on day 4, 7, 11, 14, 18, 21, 25 and 28.
Litsea cubeba was biodegraded by 71.2 % at day 14 and completely biodegraded at day 28. However as there was complete biodegradation, the test substance is not considered to inhibit mcirobial activity. It can therefore be concluded that Litsea cubeba is non-toxic to microorganisms at the nominal tested concentration of 14.2 mg/L which is therefore considered the NOEC.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 14.2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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