Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 701-039-2 | CAS number: 156324-78-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-04-15
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- GLP study according to OECD 209 (1984): only one concentration was tested, which corresponds to the highest test concentration that could be prepared due to the limited solubility of the test material in water
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- (1984)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- singed 1994-03-16
- Analytical monitoring:
- no
- Details on sampling:
- not applicable
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct dispersion in water
- Differential loading: No, 100 mg test item/L tested in triplicate
- Controls: yes, one control in duplicate - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Method of cultivation: None, direct use on the day of collection
- Preparation of inoculum for exposure: 16 mL of synthetic sewage and 250 mL of the 200 mg/L stock solution were dispersed in a 500 mL conical flask. The volume was then made up to 300 mL and 200 mL inoculum were added.
- Pretreatment: THe pH was equal to 7.1 and the suspended solids equal to 3.7 g/L, continuous aeration - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Hardness:
- dilution water: 100 mg/L as CaCO3
- Test temperature:
- 21 °C
- pH:
- 7.1
- Dissolved oxygen:
- no data; continuous aeration
- Nominal and measured concentrations:
- Nominal concentration: 0 (control), 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL conical flask filled with 300 mL
- Aeration: continuous aeration
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 2
- No. of vessels per abiotic control (replicates):
- Sludge concentration: 3.7 g/L
- Nutrients provided for bacteria: synthetic waste water according to OECD 209
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water was used; synthetic sewage (according to OECD 209) was added to the activated sewage
OTHER TEST CONDITIONS
- Photoperiod: normal laboratory light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The rate of respiration for each culture was measured for a 10-minute period after 30 minutes and 3 hours contact time.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable, limit test
- Justification for using fewer concentrations than requested by guideline: limit test with the highest test concentration that could be prepared due to the limited solubility of the test material in water. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 of 8.0 mg/L (3 h) - Reported statistics and error estimates:
- The percentage inhibition was plotted against the concentration and the EC50 values were derived by inspection for 30 minutes and 3 hours contact time.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance showed an inhibition of 16.7 % ± 3.6 % at a test item concentration of 100 mg/L after 3 hours, which corresponds to the highest test concentration that could be prepared due to the limited solubility of the test material in water. The 3-hour EC50 value for inhibition of respiration was therefore determined to be >100 mg/L.
- Executive summary:
The inhibition of respiration of activated sewage sludge by the test item was investigated in a 3-hour static test under GLP according to OECD 209 (1984). The substance showed an inhibition of 16.7 % ± 3.6 % at a test item concentration of 100 mg/L after 3 hours, which corresponds to the highest test concentration that could be prepared due to the limited solubility of the test material in water. The 3-hour EC50 value for inhibition of respiration was therefore determined to be >100 mg/L.
Reference
Oxygen consumption rates and percentage inhibition values
|
Concentration (mg/L) |
02consumption rates (mg 02/L/min) |
% inhibition |
30 minutes
|
Control R1 R2 |
0.47 0.46 |
- - |
Test item 100 R1 100 R2 100 R3 |
0.40 0.40 0.37 |
14 14 20 |
|
3,5-dichlorophenol 3.2 10 32 |
0.43 0.23 0.09 |
8 51 51 |
|
3 hours |
Control R1 R2 |
0.42 0.38 |
- - |
Test item 100 R1 100 R2 100 R3 |
0.35 0.33 0.32 |
13 18 20 |
|
3,5-dichlorophenol 3.2 10 32 |
0.33 0.14 0.06 |
18 65 85 |
R1and R3= Replicates 1 – 3.
Description of key information
The 3-hour EC50 value for inhibition of activated sludge was estimated with >100 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
The inhibition of respiration of activated sewage sludge by the test item was investigated in a 3-hour static test under GLP according to OECD 209 (1984). The substance showed an inhibition of 16.7 % ± 3.6 % at a test item concentration of 100 mg/L after 3 hours, which corresponds to the highest test concentration that could be prepared due to the limited solubility of the test material in water. The 3-hour EC50 value for inhibition of respiration was therefore determined to be >100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.