trisodium bis[3-[1-(3-chlorophenyl)-5-(hydroxykO)-3-methyl-1H-pyrazol-4-yl]diazenyl}-4-(hydroxy-kO)5-nitrobenzenesulfonato(3-)]chromate(3-)

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment.

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

Sludge from a Husman laboratory apparatus

GLP compliance:

yes

Remarks:

Quality Assurance Certificate attached to the experimental report

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

Test concentration: 1; 3.2; 10; 32 and 100 mg/l

Test organisms (species):

activated sludge

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Reference substance (positive control):

yes

Remarks:

3,5 dichlorophenol (RIEDEL DE HAEN AG, No 62493)

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Results with reference substance (positive control):

Positive control: 3,5- dichlorophenol
C50 positive results: 15 mg/l

Validity criteria fulfilled:

yes

Conclusions:

The tested substance apparead to be biodegradate by the active sludge based on the mass balance data.

Executive summary:

Based on the results of the test performed following OECD 209, the IC50 value is > 100 mg/l.

Description of key information

IC50 value is > 100 mg/l

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L