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EC number: 807-619-2 | CAS number: 65535-29-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- TEST ORGANISMS:
- Type: Activated sewage sludge bacteria
- Origin: aeration stage of the HRC sewage treatment plant treating predominantly domestic sewage
- Date of collection: 1993-03-18
PRE-TREATMENT OF THE INOCULUM:
- The sample was allowed to settle for approx. 30 minutes and the supernatant filtered through Whatman GFA coarse filter paper (first 250 mL discarded)
- The filtrate was maintained under aeration and used within 24 hours of collection - Duration of test (contact time):
- 28 d
- Initial conc.:
- 3 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- STUDY PERIOD:
- Start of experimental phase: 1993-03-18
- End of experimental phase: 1993-04-15
PRE-TREATMENT OF THE TEST SUBSTANCE:
- The test substance was dissolved in chloroform to give a stock solution of 56 mg/mL
- 10 µL aliquots of stock solution were placed on individual pieces of Whatman GFA glass filter paper and the solvent allowed to evaporate to dryness
- One piece of paper was placed in each test bottle prior to fllling with inoculated medium
- Filter paper blanks were prepared in the same manner using solvent only
PRE-TREATMENT OF THE REFERENCE COMPOUND:
- Reference substance standards (3 mg/L) were prepared by dissolving the sample directly in nutrient medium
TEST SERIES:
- Non-inoculated nutrient medium
- Inoculated nutrient medium
- Inoculated nutrient medium plus filter paper
- 2 mg/L test substance plus filter paper
- 3 mg/L sodium benzoate
- 2 mg/L test substance plus filter paper and 3 mg/L sodium benzoate (Toxicity control)
EXPOSURE CONDITIONS:
- Test volume: 250 mL
- Test apparatus: Yellow Springs BOD Meter (Model 59)
- Test performed in closed vessels: Yes
- Incubation temperature: 20 ± 1 °C
SAMPLING
- Sampling frequency: oxygen determination per bottle at day 0, 5, 15 and 28 (duplicate bottles at each sampling time)
CONTROL AND BLANK SYSTEM
- Inoculum blank: Blanks with inoculum and without test item were run - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- (Batch no. 30055292 (Fisons))
- Parameter:
- % degradation (O2 consumption)
- Value:
- 11
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 8
- Sampling time:
- 15 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 11
- Sampling time:
- 28 d
- Details on results:
- There was no evidence of inhibitory effects under the conditions of this test .
- Parameter:
- ThOD
- Value:
- 1.67 mg O2/g test mat.
- Remarks on result:
- other: Estimated oxygen depletion for 3 mgL test item
- Results with reference substance:
- Sodium benzoate attained 86% biodegradation after 28 days .
- Validity criteria fulfilled:
- yes
- Remarks:
- ready biodegradation of reference compound > 60 % within 14 days; toxicity control exhibited degradation rates > 25 % within 14 days; replicates difference < 20 %; oxygen uptake of the inoculum blank was <= 60 mg/L; no pH influence
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Epoxid-Valerat showed 11 % degradation within 28 days. Therefore the substance is not readily biodegradable. The reference compound sodium benzoate showed more than 70 % degradation after 5 days .
- Executive summary:
The study was conducted in accordance with OECD Guideline for Testing of Chemicals No. 301 D and EEC Methods for the Determination of Ecotoxicity, EEC Directive 67/548 Annex V Part C.6 (84/449/EEC). The test substance Epoxid-Valerat, dissolved in inorganic nutrient medium (concentration: 2 mg/L) and inoculated with activated sewage sludge bacteria, was incubated in duplicate in sealed bottles for 28 days at 20 ± 1°C. On days 0, 5, 15, and 28 duplicate bottles were taken and the concentration of dissolved oxygen was measured using an oxygen probe. Percent biodegradation values were calculated by comparing the oxygen depletion with the theoretical oxygen demand. The biodegradation was also studied in bottles containing sodium benzoate alone as reference compound (3 mg/) as well as test item (2 mg/L) plus sodium benzoate (3 mg/L) for investigation of inhibitory effects of the test substance, all under the same conditions. Epoxid-Valerat showed only up to 11 % degradation. There was no indication of inhibitory effects caused by the test compound. The reference compound showed more than 70 % degradation after 5 days. In conclusion Epoxid-Valerat is not readily biodegradable.
Reference
- Cultures containing both test and standard substances combined showed an oxygen depletion value 1 % higher than that anticipated on the basis of results from separate cultures. Consequently the test item was not considered to have had an inhibitory effect on sewage bacteria under the conditions of this test.
- Oxygen depletions in the inoculated (<0.5 mg O2/L after 5 days and <0.6 mg O2/L after 28 days) and non-inoculated control (<0.3 mg O2/L after 5 days and <0.4 mg O2/L after 28 days) series were within the prescribed limits.
Description of key information
The substance showed 11 % degradation within 28 days. The reference compound showed more than 70 % degradation after 5 days. The substance is considered to be "Not Readily Biodegradable".
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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