Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-678-4 | CAS number: 1076-12-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 December 2011 to 24 July 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 10, 30, 100, 300 and 1000 mg/L.
- Sampling method: Their rates of oxygen consumption were determined using oxygen electrodes and were compared with those of four replicate controls that contained activated sludge and synthetic sewage alone.
- Sample storage conditions before analysis: sampled in situ - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: On the day of collection of the activated sludge, an aliquot (10 mL) of the activated sludge was filtered through a dried and preweighed Whatman GF/C filter paper, which was then dried again at approximately 105°C for at least one hour, allowed to cool in a desiccator and reweighed. The mixed liquor suspended solids (MLSS) content of the activated sludge was then calculated. Synthetic sewage (50 mL/L) was added to the stock of activated sludge and this was aerated overnight.
- Eluate: Reverse osmosis water
- Controls: The method of preparation used during the definitive test was based on the results of a formulation trial. Volumes of the test substance (0.0049, 0.0147, 0.049, 0.147 and 0.490 mL, corrected for a specific gravity of 1.02) were added to test bottles (500 mL), made up to volume as appropriate, to give four replicates of test solutions, with nominal concentrations of 10, 30, 100, 300 and 1000 mg/L. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: No
- Method of cultivation: On the day of collection, an aliquot (10 mL) of the activated sludge was filtered through a dried and preweighed Whatman GF/C filter paper, which was then dried again at approximately 105°C for at least one hour, allowed to cool in a desiccator and reweighed.
The mixed liquor suspended solids (MLSS) content of the activated sludge was then calculated. Synthetic sewage (50 mL/L) was added to the stock of activated sludge and this was aerated overnight.
- Preparation of inoculum for exposure: On the day of the test, the MLSS content of the sludge was determined (in triplicate) and adjusted to 4 g/L by the addition of tap water. The pH and temperature of the sludge were also measured. Aliquots (200 mL) were then added to each mixture to give a final MLSS concentration of 1.6 g/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- None
- Hardness:
- No data. The dilution water used to prepare solutions of test mixtures, synthetic sewage and the reference substance was reverse osmosis (RO) water.
- Test temperature:
- 20 ± 2°C.
- pH:
- 7.5 ± 0.5
- Dissolved oxygen:
- No data. The dilution water used to prepare solutions of test mixtures, synthetic sewage and the reference substance was reverse osmosis (RO) water.
- Salinity:
- No data. The dilution water used to prepare solutions of test mixtures, synthetic sewage and the reference substance was reverse osmosis (RO) water.
- Nominal and measured concentrations:
- Test substance nominal concentrations: 10, 30, 100, 300 and 1000 mg/L.
Reference substance: 3, 10, 32 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL bottles
- Type: closed
- Material, size, headspace, fill volume: no data
- Aeration: no
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per reference (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reverse osmosis water
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 3
- Range finding study: no
- Test concentrations: 10, 30, 100, 300 and 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: Reference substance; 3,5-DCP
- Details on results:
- The no observed effect concentration (NOEC) for TCD was 100 mg/L. The EC20, EC50 and EC80 values could not be calculated, although there was significant inhibition of respiration rate at test concentrations of 300 (26%) and 1000 mg/L (40%).
- Results with reference substance (positive control):
- - Results with reference substance valid: Yes, the three-hour EC50 for 3,5-DCP (2.22 mg/L) fulfilled the validity criterion relating to sensitivity to inhibition (acceptable EC50 range 2 to 25 mg/L).
- Reported statistics and error estimates:
- Where possible, the EC20, EC50 and EC80 (and 95% confidence intervals) and no observed effect concentration (NOEC) of the test substance were calculated using SAFEstat programme SAS Proc NLIN (SAS Institute, 2002).
For the 3,5-dichlorophenol reference substance, only the EC50 was calculated.
The EC50 (and EC20 and EC80) was the concentration of the test or reference material at which the respiration rate is 50% of mean control value (or 20% and 80% of the mean control value).
For substances that do not significantly inhibit the activity of the sludge, the no observed effect concentration (NOEC) will be taken as the highest concentration tested.
TCD and 3,5-Dichlorophenol mixtures were analysed separately. - Validity criteria fulfilled:
- yes
- Conclusions:
- The no observed effect concentration (NOEC) for TCD was 100 mg/L. The EC20, EC50 and EC80 values could not be calculated, although there was significant inhibition of respiration rate at test concentrations of 300 (26%) and 1000 mg/L (40%).
Reference
Inhibition for TCD
10 mg/L 8%
30 mg/L 12%
100 mg/L 18%
300 mg/L 26%
1000 mg/L 40%
Inhibition for 3,5 -DCP
3 mg/L 57%
10 mg/L 55%
32 mg/L 80%
Description of key information
The no observed effect concentration (NOEC) for TCD was 100 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
In a test carried out in accordance with OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (carbon and ammonium oxidation), the test substance showed significant inhibition of respiration rate at test concentrations of 300 (26%) and 1000 mg/L (40%). However EC values could not be determined as there was not enough evidence to identify the concentration-response relationship.
The ‘no observed effect concentration’ (NOEC) for TCD was determined as 100 mg/L, and the ‘lowest observed effect concentration’ (LOEC) was 300 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.