Cyclopropanecarbonitrile, 1-amino-, hydrochloride (1:1)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12 Dec.2011 - 17 Feb.2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Biodegradation is determined by the EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-Amino-1-cyclopropanecarbonitrile hydrochloride

EC Number:

629-718-8

Cas Number:

127946-77-4

Molecular formula:

C4H7N2Cl

IUPAC Name:

1-Amino-1-cyclopropanecarbonitrile hydrochloride

Constituent 2

Reference substance name:

1-aminocyclopropane-1-carbonitrile hydrochloride

IUPAC Name:

1-aminocyclopropane-1-carbonitrile hydrochloride

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Activated sludge from a bilogical sewage treatment plant was used. The chosen plant treats mostly domestic sewage. The sludge was taken from the activation basin of the ESn (Stadtentsorgung Neustad) sewage treatment plant.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

424.88 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Reference substance

Reference substance:

aniline

Remarks:

624.2mg/L in desionised water

Results and discussion

Test performance:

Six samples were taken in order to determinate the DOC (after 3 hours and on days 2, 6, 9, 16 and 28). DOC was determinated as difference between Total Carbon and Inorganic Carbon using a carbon analyser (Analytik Jena).

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

-1

Sampling time:

2 d

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

-0.5

Sampling time:

6 d

% Degradation 3

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

9 d

% Degradation 4

Parameter:

% degradation (DOC removal)

Value:

38.6

Sampling time:

16 d

% Degradation 5

Parameter:

% degradation (DOC removal)

Value:

92.1

Sampling time:

28 d

Details on results:

Ready degradability is defined in the guideline as degradation surpassing 70% within 10 days after reaching a level of 10%. Therefore, the test item cannot be considered as "readily biodegradable". Degradation surpassed 70% within 28 days, though. Therefore, the test item may be considered as biodegradable within 28 days either. Therefore test item can be considered as "biodegradable".

BOD5 / COD results

Results with reference substance:

Degradation of the positive control was 100% after six days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: biodegradable

Conclusions:

1-Cyanocyclopropanaminium chloride is biodegradable.