4-[trans-4-(trans-4-butylcyclohexyl)-cyclohexyl]-1-ethoxy-2, 3-difluorobenzene

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 2011-06-08 to 2011-08-01

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch: E011009938

Analytical monitoring:

yes

Details on sampling:

0; 24, 48, 72, 96 h

Vehicle:

yes

Details on test solutions:

According to guideline
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): TWEEN 80 / SPAN 80 (3/1 v/v)
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)):

- Nominal values

Substance Concentration Solubilizer Tween 80 / SPAN-80
pos. Control 0.05 mg/L -
pos. Control 0.5 mg/L -
Test item: 33 mg/L 100 mg/L

Vehilce
Control: - 100 mg/L

Negative
Control - -

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Weight: 0.33 +/- 0.03 g
Length: 3.4 +/- 0.1 cm
Lot: 292-1
Date: 2011-06-08

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Test temperature:

25 +/- 0.1 °C

Nominal and measured concentrations:

33 mg/L

Details on test conditions:

10 fish per 4 L

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 33 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: with vehicle Tween 80 / Span 80 (3/1)

Sublethal observations / clinical signs:

- Mortality of control: 0 % after 96 hours

Mortality in %

Sample		Mortality / [%]
Test Material	Concentration / [mg/L]	24 h	48 h	72 h	96 h
Positive Control	0.5	20	70	90	100
Positive Control	0.05	0	0	0	0
Test item (+vehicle)	33	0	0	0	0
Tween 80 / SPAN-80 (3/1)	100	0	0	0	0
Blank	0	0	0	0	0

All concentrations tested were at or above the solubility limit of the pure test substance. To achive the exposure concentrations, solubiliser was used.

Validity criteria fulfilled:

yes

Conclusions:

The LC50 of the test material exceeds 33 mg/L and is clearly above the limit of water solubility.

Executive summary:

The informtion for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 203. The LC50 of the test material exceeds 33 mg/L and is clearly above the limit of water solubility.

Description of key information

GLP OECD 203 Assay: LC50 > 33 mg/L, NOEC = 33 mg/L (measured initial) with solubilizer

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

33 mg/L

Additional information

The LC50 of the test material exceeds 33 mg/L and is clearly above the limit of water solubility.