kresoxim-methyl (ISO);methyl (E)-2-methoxyimino-[2-(o-tolyloxymethyl)phenyl]acetate

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Following national standard

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate

EC Number:

604-351-6

Cas Number:

143390-89-0

Molecular formula:

C18 H19 N O4

IUPAC Name:

methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate

Details on test material:

- Name of test material (as cited in study report): Reg. No. 242 009
- Analytical purity: 94%
- Batch No.: N27

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared by adding 1330 mg Reg. No. 242 009 in 1000 ml sterile water (containing 0.01% (w/v) Tween 80).
- Chemical name of vehicle: Tween 80

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: Berlin 33/2
- Source: German Collection of Microorganisms), Braunschweig, Germany
- Method of cultivation: The strain was preserved on slant agar culture tubes according to DIN 38412. The pre-culture and nutrient solutions I to IV were also prepared in accordance with this DIN standard.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Test conditions

Test temperature:

21 °C

Nominal and measured concentrations:

Nominal test concentrations: control, 50, 100, 250, 500, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 10 ml of a Pseudomonas suspension adjusted to a turbidity of 50 TE/F

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412

EFFECT PARAMETERS MEASURED: O2 consumption in a Sapromat apperatus over a period of 16 hours

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

16 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC50 (16 h) = 7.2 mg/L
EC10 (16 h) = 2.75 mg/L