kresoxim-methyl (ISO);methyl (E)-2-methoxyimino-[2-(o-tolyloxymethyl)phenyl]acetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-12-08 - 1992-12-10

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate

EC Number:

604-351-6

Cas Number:

143390-89-0

Molecular formula:

C18 H19 N O4

IUPAC Name:

methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate

Details on test material:

- Name of test material (as cited in study report): Reg 242 009 techn.
- Physical state: powder
- Analytical purity: 93.7% active ingredient
- Batch No.: N 36

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The stock solution of the test substance was freshly prepared with M4-medium. The concentration of the stock solution was 1.096 mg/L. By dilution of this stock solution with test medium (M4 - medium pH 7) the nominal concentrations were prepared.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF
- Age at study initiation: 2-24 h
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.45 mmol/L

Test temperature:

19.6 - 20.4 °C

pH:

7.0 - 7.8

Dissolved oxygen:

8.0 - 9.0 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 0.004, 0.009, 0.017, 0.034, 0.069, 0.137*, 0.274, 0.548*, 1.096 mg/L
* = analytically analysed concentrations
Recovery rate of the analytically analysed concentrations: 90.4-94.7% (without daphnids, 0 h), 85.3-93.6% (without daphnids, 48 h), 87.3-93.6% (with daphnids, 48 h)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, test volume: glass tubes with flat bottom, 20 ml, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium M4 prepared of the basis of an ultrapure, deionized water.
- Total hardness: 2.7 ± 0.5 mmol/L
- Alkalinity up to pH 4.3: 0.9 ± 0.1 mmol/L
- Ca/Mg ratio: about 4:1
- Conductivity: 600 - 700 µS/cm
- Intervals of water quality measurement:
- Temperature: after 0, 24, 48 h in an extra vessel
- pH and oxygen content: after 0 and 48 h in one parallel at each concentration

OTHER TEST CONDITIONS
- Photoperiod: artificial light, day:night rhythm 16:8 hours
- Light intensity: about 5-6 µE/(m²s) in the range of 400 - 700 nm

EFFECT PARAMETERS MEASURED: Mobility after 0, 3, 6, 24, 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.069 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.186 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 0.161-0.216 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.548 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: none

- Effect concentrations after 3 h:
EC0 = 0.274 mg/L
EC50 = 0.940 mg/L
EC100 = >1.096 mg/L

- Effect concentrations after 24 h:
EC0 = 0.137 mg/L
EC50 = 0.428 mg/L
EC100 = 0.1.096 mg/L