Registration Dossier
Registration Dossier
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EC number: 619-817-4 | CAS number: 52671-72-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31.07.1985 - 30.08.1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The Zahn-Wellens test was conducted according to national standard (DIN 38 412, Part 25, 1984), which is similar to OECD 302B; limited documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- yes
- Remarks:
- In the today's methods no industrial but only municipal wastewater sludge is used and the concentrations of test substance and sludge are some lower
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2,4-dioxo-3-oxatricyclo[7.3.1.0⁵,¹³]trideca-1(13),5,7,9,11-pentaene-8,10-dicarboxylic acid
- EC Number:
- 619-817-4
- Cas Number:
- 52671-72-4
- Molecular formula:
- C14H6O7
- IUPAC Name:
- 2,4-dioxo-3-oxatricyclo[7.3.1.0⁵,¹³]trideca-1(13),5,7,9,11-pentaene-8,10-dicarboxylic acid
Constituent 1
Study design
- Oxygen conditions:
- not specified
- Inoculum or test system:
- activated sludge, industrial (adaptation not specified)
- Duration of test (contact time):
- 20 d
Initial test substance concentration
- Initial conc.:
- ca. 272 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Preparation of the stock solution: 10 g/L test substance was stirred in water. The resulting suspension was filtered through a folded paper filter, the filtrate was used as stock solution.
Reference substance
- Reference substance:
- diethylene glycol
- Remarks:
- 271 mg DOC/L
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 87
- Sampling time:
- 28 d
- Remarks on result:
- other: 272 mg/L test substance
- Parameter:
- % degradation (DOC removal)
- Value:
- 54
- Sampling time:
- 28 d
- Remarks on result:
- other: 544,8 mg/L test substance
- Details on results:
- Inherent and complete biodegradability was achieved in the lower concentrated test batch (272 mg/L test substance). With the double concentrated batch biodegradation was slow and incomplete after 28 days.
BOD5 / COD results
- Results with reference substance:
- 1 d: 17 %
5 d: 58 %
7 d: 97 %
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- The test item is inherently and completely biodegradable (test batch 272 mg/L test substance)
- Executive summary:
Testing of tetra acid for inherent biodegradability was performed in the Zahn-Wellens test. The results of testing revealed that the lower concentrated test batch is inherently and completely biodegradable. The degradation of the reference substance was in the demanded range and caused a DOC reduction of 97 % within 7 days.
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