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EC number: 700-144-0 | CAS number: 13115-71-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-09-28 to 2009-10-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 23 March, 2006
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- 24 August, 2009
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 6.25, 25 and 100 mg/L after 0, 24, 48 and 72 h
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a stock solution with a concentration of 1 g a.i./L was prepared. The required amount of test material was added to the test medium and stirred for 15 minutes. The test concentrations wer prepared separately by dilution of the stock solution. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: 86.81
- Source: Obtained from "Sammlung von Algenkulturen", Göttingen (SAG), (Collection of Algae Cultures, Institute for Plant physiology), University of Göttingen, Germany
- Method of cultivation: cultured at 25 ± 1 °C and 8000 ± 1000 Lux
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24.9 - 25.1 °C
- pH:
- Control: 8.13 (start) - 9.30 (end)
Treatment: 7.42 - 7.83 (start), 8.61 - 10.26 (end) - Nominal and measured concentrations:
- Nominal test substance concentrations: control, 6.25, 12.5, 25, 50, 100 mg a.i./L
Recovery rate of DOC: 88 - 99% - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlendmeyer flasks (300 mL) with cotton-wool plugs
- Material, fill volume, headspace: glass, 150 mL, 150 mL
- Initial cells density: 5000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: prepared according to OECD 201
- Intervals of water quality measurement: pH at start end end of exposure period, temperature daily
OTHER TEST CONDITIONS
- Light intensity and quality: 8000 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
- Determination of cell concentrations: photometrically (spectrophotometer at a wavelength of 578 nm) after 0, 24, 48 and 72 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes (Test conducted 2009-04-17 to 2009-04-23)
- EC50: ErC50 (72 h): 0.9 mg/L - Reported statistics and error estimates:
- Statistical evaluation was performed using ToxRat® professional.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results of this study show that the test substance Glycyl-L-glutamine monohydrate induced no inhibition of algal growth after 72 h of exposure. For the parameter yield the EyC50 value was >100 mg a.i./L and the LOEC and NOEC values were >100 mg a.i./L and ≥100 mg a.i./L, respectively, after 72 h of exposure, based on nominal concentrations. For the parameter growth rate the ErC50 value was >100 mg a.i./L and the LOEC and NOEC values were >100 mg a.i./L and ≥100 mg a.i./L, respectively, after 72 h of exposure, based on nominal concentrations.
Reference
Description of key information
ErC10/50 (72 h) > 100 mg/L (nominal, OECD 201)
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
The toxicity of the substance to aquatic algae was investigated in a study following OECD 201 (GLP). The study was conducted using Desmodesmus subspicatus as test organism. The algae were exposed to nominal test substance concentrations of 6.25, 12.5, 25, 50, 100 mg a.i./L including a control for 72 hours. The test substance concentration was analytically verified by DOC analysis and the recovery rate was 88 - 99% of nominal. An inhibition of algal growth was not observed. An ErC10/50 (72 h) > 100 mg/L was determined based on growth rate of algal cells.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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