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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Nov 2004 - Mar 2005
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
-
EC Number:
473-690-8
EC Name:
-
Cas Number:
738602-93-2
Molecular formula:
not applicable for UVCB
IUPAC Name:
(2R,3R,4S,5S,6R)-2-{[(2R,3R,4R,5S,6R)-5-{[(2R,3R,4R,5S,6R)-3,4-dihydroxy-6-(hydroxymethyl)-5-{[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}oxan-2-yl]oxy}-3,4-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-6-(hydroxymethyl)oxane-3,4,5-triol; (2S,3R,5R)-4-{[(2R,3R,4R,5S,6R)-5-{[(2R,3R,4R,5S,6R)-3,4-dihydroxy-6-(hydroxymethyl)-5-{[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}oxan-2-yl]oxy}-3,4-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}hexane-1,2,3,5,6-pentol
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material: Sponsor, Batch 4G08
- Purity, including information on contaminants, isomers, etc.: 54.2 % Maltotriosyl glucoside


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature at about 20 °C away from direct sunlight

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
The study was performed with aerobic activated sludge from a wastewater treatment plant (ARA Ergolz II, Füllinsdorf, Switzerland) treating predominantly domestic wastewater.
- Laboratory culture: no laboratory culture
- Method of cultivation: no cultivation
- Storage conditions: no storage
- Storage length: no storage
- Preparation of inoculum for exposure:
The sludge was washed tice with tap water by centrifugation and the superantant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated.
- Pretreatment: washing
- Concentration of sludge: 4 g dry mass per litter
- Initial cell/biomass concentration: no determination of biomass
- Water filtered: no
- Type and size of filter used, if any:
Duration of test (contact time):
14 d
Initial test substance concentration
Initial conc.:
75.2 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: according to guidelines
- Additional substrate: non
- Solubilising agent (type and concentration if used): none
- Test temperature: 23 °C
- pH: 7.4
- pH adjusted: yes, at start of test only
- CEC (meq/100 g): not applicable
- Aeration of dilution water: no
- Suspended solids concentration: not determined
- Continuous darkness: yes
- Other:

TEST SYSTEM
- Culturing apparatus: no culturing apparatus used
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: no special method used
- Method used to create anaerobic conditions: no special method used
- Measuring equipment: TOC Analyzer equipped with an autosampler
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes, loosely covered flasks
- Details of trap for CO2 and volatile organics if used: not used
- Other:

SAMPLING
- Sampling frequency: on day 0, 3, 7, 10 and 14
- Sampling method: 10 ml of sample were taken
- Sterility check if applicable: not stated
- Sample storage before analysis: no
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
- Other:

STATISTICAL METHODS: none applied
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

Test performance:
no unusual observations reported
% Degradation
Key result
Parameter:
% degradation (DOC removal)
Value:
94
Sampling time:
14 d
Details on results:
In the test flasks, containing the test item and activated sludge (inoculum), the mean concentration of DOC (dissolved organic carbon) rapidly decreased from initially 30.1 mg/L on Day 9 to 1.9 mg/L on Day 3.
In the abiotic control, containing the test item and poisoned medium, the DOC concentration varied from 33.3 to 31.8 mg/L over the exposure period of 14 days and were not significantly different from the initial DOC concentration of 33.2 mg/L measured on Day 0. Thus no significant abiotic elimination occured under the test conditions.
The reference item was completely biodegraded within the first three days of exposure, thus confirming suitability of the activated sludge.
In the toxicity control, containing the test item, the reference item and activated sludge, the initial DOC concentraiton of 59.4 mg/L measured on Day 0 decreased to 2.1 mg/L on Day 14. Biodegradation amounted to 97 % within 14 days of exposure. Thus, according to the test guidelines, it is assumed that the test item is not inhibitory to activated sludge at the test concentration of 75 mg/L because degradation was clearly >35 % within 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
MG-60 is readily biodegradable under the test conditions.
Executive summary:

The test item MG-60 was investigated for its ready biodegradability in a 28 Day DOC Die-Away test" according to EU C.4-A and OECD 301 A. In accordance with the testing guidelines, the test was ended ahead of schedule after 14 days of exposure, because the biodegradation curves of the test and reference item had reached a plateau for three determinations.


In the test flasks, containing the test item MG-60 and activated sludge (inoculum), the mean concentrations of dissolved organic carbon (DOC) rapidly decreased by 94 % of the initial values within the first three days of the test period. Therefore, MG-60 was found to be highly biodegradable under the test conditions, an dthe pass level for ready biodegradability, i.e. 70 % DOC removal in a 10-day window within a 28-day test period, was reached. Consequently, the test item MG-60 is readily biodegradable under the test conditions.


In the abiotic control, containing the test item and poisoned mineral medium, no significant elimination was noted after 14 days of exposure (based on DOC measurements).


The reference item sodium benzoate was completely biodegraded within the first three days of exposure, thus confirming suitability of the activated sludge.


In the toxicity control, containing the test item, hte reference item sodium benzoate and activated sludge (inoculum), the initial DOC decreased by 97 % within 14 days of exposure. According to the test guidelines, it is assumed that the test item is not inhibitory to activated sludge at the tested concentration of 75 mg/L, because degradation was > 35 % within 14 days.