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EC number: 428-310-5 | CAS number: 225789-38-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- stirring (magnetic stirrer) for 24 h; adjusted to pH 7.0 to 7.2 and filtered;
- Vehicle:
- yes
- Details on test solutions:
- test water according to Elendt, M4; clear solution; no visible particulate matter after filtration;
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- feed three times a week with green algae; daphnids with an age < 24 h
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- mmol Ca2+ + Mg2+/l: 2.1 - 2.7
- Test temperature:
- 21°C
- pH:
- 7.0 - 7.2
- Dissolved oxygen:
- 8.0 - 8.8 mg/l
- Nominal and measured concentrations:
- nominal 1mg/l; mean 1,014 mg/l
nominal 3,2mg/l; mean 3,250 mg/l
nominal 10mg/l; mean 10,049 mg/l
nominal 32mg/l; mean 31,907 mg/l
nominal 100mg/l; mean 98,410 mg/l - Details on test conditions:
- 100 ml beakers
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 46.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 10 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 32 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- reproduction
- Details on results:
- All test animals in the 100 mg/ group died within 4 days. At 32 mg/l a mortality rate of 80% was observed. Daphnids of this group died between days 14 - 17 of the study. In the 1, 3.2 and 10 mg/l groups as well in the control group no mortality occurred.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this reproduction toxicity study with the test item in Daphnia magna over 21 days a NOECD (reproduction and mortality) of 10 mg/L were obtained. The EC10 reproduction is 12.8 mg/L, the EC10 mortality is 11.3 mg/L.
- Executive summary:
The test item was tested in a daphnia magna reproduction study over 21 days in a semistatic system. The concentrations tested were 1, 3.2, 10, 32, 100 mg/L and 0 mg/L (control).
The test was conducted under semistatic conditions. Therefore, the test solutions were renewed every Monday, Wednesday and Friday using freshly prepared test solutions. The corresponding preparations were stirred for approx. 24 hours with a magnetic stirrer. To further improve solubility and stability of the test substance in the test medium, all test batches were adjusted to a pH range of 7.0 – 7.2 after the stirring period and run through a filter of 0.2 µm pore size.
All test solutions were present as clear solutions. There were no visible particulate matters. Water parameters were within the normal range.
Analytical monitoring of the concentrations of the test substance in the test medium was carried out during three test intervals. Sampling was performed at the start and the end of the interval. Two 48 hours- and one 72 hours interval were surveyed. The values determined were in a range of +/- 10% of the theoretical value.
In the control group, no mortality occurred and the mean number of living offspring produced per parent animal was 80.1, thus fulfilling the validity criteria for the test.
All test animals in the 100 mg/L group died within 4 days of exposure. At 32 mg/L a mortality rate of 80 % was observed. Daphnids of this group between days 14 – 17 of the study. In the 1, 3.2 and 10 mg/L groups, as well as in the control group, no mortality occurred.
Surviving animals of all concentration groups showed no difference in the onset of brood production in comparison to the control.
The reproduction rate in the 1-10 mg/L concentration groups showed no statistically significant changes in comparison to the control.
In this reproduction toxicity study with the test item in daphnia magna over 21 days the following effect concentrations were obtained:
Reproduction:
EC10 = 12.8 mg/L
EC50 = 46.2 mg/L
NOEC = 10 mg/L
Mortality
EC10 = 11.3 mg/L
EC50 = 22.3 mg/L
NOEC = 10 mg/L
Reference
Description of key information
In this reproduction toxicity study with the test item in Daphnia magna over 21 days a NOECD (reproduction and mortality) of 10 mg/L were obtained. The EC10 reproduction is 12.8 mg/L, the EC10 mortality is 11.3 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 10 mg/L
Additional information
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