2-(4-(diethylaminopropylcarbamoyl)phenylazo)-3-oxo-N-(2,3-dihydro-2-oxobenzimidazol-5-yl)butyramide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Sept - Nov 1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

1984

Deviations:

yes

Remarks:

generated CO2 was captured by sodium hydroxide instead of barium hydroxide for analytical improvement

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: guideline 3/449/EWG part C

Deviations:

yes

Remarks:

see OECD 301 B

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-(4-(diethylaminopropylcarbamoyl)phenylazo)-3-oxo-N-(2,3-dihydro-2-oxobenzimidazol-5-yl)butyramide

EC Number:

404-910-2

EC Name:

2-(4-(diethylaminopropylcarbamoyl)phenylazo)-3-oxo-N-(2,3-dihydro-2-oxobenzimidazol-5-yl)butyramide

Cas Number:

164578-14-7

Molecular formula:

C25H31N7O4

IUPAC Name:

N-[3-(diethylamino)propyl]-4-[(1E)-2-{2-oxo-1-[(2-oxo-2,3-dihydro-1H-1,3-benzodiazol-5-yl)carbamoyl]propyl}diazen-1-yl]benzamide

Test material form:

solid: bulk

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): communal sewage plant, Franfurt-Sindlingen
- Preparation of inoculum for exposure: none
- Pretreatment: none

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

10 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

test mat.

Initial test substance concentration 3

Initial conc.:

6.09 other: mgC/L

Based on:

DOC

Initial test substance concentration 4

Initial conc.:

12.17 other: mgC/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: according to EEC Directive Section 1.6.4.2
- Additional substrate: none
- Test temperature: 22 +/- 2°C (airconditioned room)
- pH: 7
- pH adjusted: yes, in case of refernce sample (benzoic acid)
- Aeration of dilution water: application of CO2 purified air

TEST SYSTEM
- Culturing apparatus: 5L glas bottles with magnetic stirrer
- Number of culture flasks/concentration: 4
- Method used to create aerobic conditions: CO2 purified air was lead through the test solutions
- Measuring equipment: IONICS 1270 M and IONICS Model 555 TOC/TC analyser
- Details of trap for CO2: 3 traps filled with 100 mL 0.05 M NaOHeach per culture

SAMPLING
- Sampling frequency: days 3, 7, 14, 21 and 28
- Sampling method: 6 mL per sampling time

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Reference substance

Reference substance:

other: benzoic acid

Results and discussion

% Degradation

Key result

Parameter:

% degradation (CO2 evolution)

Value:

< 20

Sampling time:

28 d

Remarks on result:

other: determined by modified Sturm test

Details on results:

Points of degradation (test substance):
1 % degradation after 28 d (6.09 mgC/L initial concentration)

2 % degradation after 14 d (12.17 mgC/L initial concentration)
3 % degradation after 21 d (12.17 mgC/L initial concentration)
4 % degradation after 28 d (12.17 mgC/L initial concentration)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The substance was experimentally determined to be not readily biodegradable.

Executive summary:

From the results obtained under the experimental conditions employed, the evaluation of the biotic degradation of the test article using the modified Sturm test showed: Biodegradation of the test article after 28 days = 4 %. Theefore, the test item can be considered as: Not readily biodegradable.