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EC number: 305-170-2 | CAS number: 94349-52-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2016-02-17 to 2016-04-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study performed according to OECD 301B.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-ethyl-4-[[4-(ethylamino)-m-tolylphenyl][4-(ethylimino)-3-methylcyclohexa-2,5-dien-1-ylidene]methyl]-m-toluidine monoacetate
- EC Number:
- 305-170-2
- EC Name:
- N-ethyl-4-[[4-(ethylamino)-m-tolylphenyl][4-(ethylimino)-3-methylcyclohexa-2,5-dien-1-ylidene]methyl]-m-toluidine monoacetate
- Cas Number:
- 94349-52-7
- Molecular formula:
- C28H38N3.C2H3O2
- IUPAC Name:
- [4-[bis[4-(ethylamino)-3-methyl-phenyl]methylene]-2-methyl-cyclohexa-2,5-dien-1-ylidene]-ethyl-ammonium;acetate
- Details on test material:
- - Chemical name: N-Ethyl-4-[[4-(ethylamino)-m-tolylphenyl][4-(ethylimino)-3-methylcyclohexa-2,5-dien-1-ylidene]methyl]-m-toluidinemonoacetate
- Physical state: solid / green
- Expiration date of the lot/batch: 01 July 2020
- Water solubility: miscible
- Storage conditions: Storage at room temperature; avoid direct sun light
- Total Organic Carbon: 734 mg/g
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal activated sludge from the wastewater treatment plant of Mannheim, Germany.
- Preparation of inoculum for exposure: A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size about 1 mm. To reduce the content of inorganic carbon in the blank controls the activated sludge was aerated with carbon dioxide free air for about 48 hours at 22 ± 2° C. At the day of exposure the suspension was washed one time with drinking water. Therefore the aeration was stopped and the sludge was allowed to settle. After settling the supernatant was discarded and the remaining sludge suspension was filled up with drinking water.
- Concentration of sludge: 6 g/L dry weight
- Water filtered: yes
- Type and size of filter used, if any: Finely woven mesh with a size about 1 mm - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
- Initial conc.:
- 10 mg/L
- Based on:
- other: TOC nominal
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The used mineral medium complies with the test guidline OECD 301B.
- Test temperature: 22 +/- 2 °C
- pH: 7.4 +/- 0.2
- pH adjusted: Yes
- Concentration of activated sludge: 30 mg/L dw
TEST SYSTEM
- Culturing apparatus: The test was performed in 2 L incubation bottles filled up to a volume of 1.5 L. The bottles were connected to two serial scrubbing bottles (total volume 250 mL) filled with 100 mL 0.05 mol sodium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes.
- Number of culture flasks/concentration: 2 blank controls and 2 flasks per test substance concentration.
- Method used to create aerobic conditions: The aeration was performed with carbon dioxide free air at a flow of approximately 800 mL per hour for about 24 hours.
- Measuring equipment: TOC-analyser equipped with an auto sampler (Shimadzu TOC-5000A and/or TOC-L (CSH/CSN))
- Details of trap for CO2: The test bottles were connected to two serial scrubbing bottles (total volume of 1.5 L). The bottles were connected to two serial scrubbing bottles (total volume 250 mL) filled with 100 mL 0.05 mol sodium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes.
SAMPLING
- Sampling frequency and method:
The TIC- and DOC-analyses were performed as repeat determination, using a TOCanalyzer equipped with an auto sampler (Shimadzu TOC-5000A and/or TOC-L CSH/CSN). The samples for the DOC-analysis were centrifuged for about 15 minutes at 4000 rpm. Measurement of Total Inorganic Carbon (TIC) of the adsorption solution was performed on day 2, 5, 8, 12, 14, 16, 19, 23, 26, 28 and used for the calculation of the produced carbon dioxide. Measurement of Dissolved Organic Carbon (DOC) was performed at beginning and at the end of exposure.
The DOC was calculated using the following formula:
TC - IC = TOC (since samples were centriuged TOC = DOC)
TC: total carbon
IC: inorganic carbon
TOC: total organic carbon
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Inhibition control: Yes
- Reference substance control: Yes
Reference substance
- Reference substance:
- aniline
Results and discussion
- Preliminary study:
- No data
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 10 - 20
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- The test substance was not readily biodegradable under the conditions of this test.
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