Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 284-915-2 | CAS number: 84989-26-4
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
IC50 (3h) > 100 mg/l
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
The toxicity to microorganisms of the Acid Brown 355b (ABr355b - EC: 280-689-4) was assessed performing a test in accordance with the OECD Test Guideline N. 209. Mixed culture of bacteria (sludge of a Husman apparatus) was collected for the experiment and the following concentration were assayed: 1, 3.2, 10, 32 and 100 mg/l. 3,5 -Dichlorophenol was used as positive control. The Inhibition Concentration (IC50) after 3 hours was identified as greater than 100 mg/l (Huntsman Textile Effects (Germany) GmbH, 1986).
The test was performed on the ABr355b (EC 280-689-4), trisodium salt: chromate(3-),[3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulfonato(3-)][3-hydroxy-4-[(2-hydroxy-1-naphthalenyl)azo]-7-nitro-1-naphthalenesulfonato(3-)]-, trisodium.
The reference substance under registration is the Acid Brown 355 (ABr355 - EC 284-915-2), the form which can have a variable number of the sodium atoms. Thus, although it has been appointed a different EC number to the ABr355b trisodium salt form (EC 280-689-4), it can be considered as “included” into the structure variability of the ABr355 (EC 284-915-2). In this context, the use of the experimental data available on the ABr355b (EC 280-689-4), in order to assess the ABr355 (EC 284-915-2), can be considered as not a read across, but an evaluation on one of the possible forms of the ABr355. Furthermore, based on the experience in the manufacture of the ABr355, the most frequent form is the trisodium salt.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
