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EC number: 256-370-0 | CAS number: 49556-16-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11.2017 to 04.2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 108
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tin(2+) neodecanoate
- EC Number:
- 256-370-0
- EC Name:
- Tin(2+) neodecanoate
- Cas Number:
- 49556-16-3
- Molecular formula:
- C20H38O4Sn
- IUPAC Name:
- λ²-tin(2+) bis(2,2-dimethyloctanoate)
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The test substance concentrations were measured via DOC measurement at time 0 and 72 h after filtration of the samples.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Due to the very low water solubility of the test item individual Water Accommodated Fractions (WAFs) of the test item will be prepared according to the OECD guidance No. 23.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Raphidocelis subcapitata
- Strain: Catalog No 61.81 SAG
- Source (laboratory, culture collection): The organism was obtained from 'Sammlung von Algenkulturen', Göttingen (SAG), Culture Collection of Algae at Pflanzenphysiologisches Institut of the University at Göttingen, Albrecht von Haller Institut, Untere Klarspüle 2, D-37073 Göttingen, Germany
- Age of inoculum (at test initiation): 3 d
- Method of cultivation:
Stock culture: The stock cultures are maintained fulfilling the criteria of the OECD guideline (culture medium recommended by Bringmann und Kühn (1980).
Pre-culture: Prior to testing a pre-culture will be established in standard OECD growth medium to obtain exponentially growing algae for the test. The culture duration of the pre-cultures was 3 days.
ACCLIMATION
- Acclimation period: 3 d
- Culturing media and conditions (same as test or not): OECD growth medium according to OECD 201 was used.
- Any deformed or abnormal cells observed: Normal appearance of algal cells at all concentrations. Some cell debris at 63.3 and 200.0 mg test iten/L (nominal concentration)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21 - 24°C (controlled at ± 2°C)
- pH:
- at test start: 7.00-7.51
at test end:7.61-7.97 - Nominal and measured concentrations:
- Control, 2.00, 6.33, 20.0, 63.3 and 200 mg test item/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL conical flasks
- Type (delete if not applicable): covered with air-permeable silicone-sponge caps
- Material, size: glas, 250 mL
- Aeration: not reported
- Type of flow-through (e.g. peristaltic or proportional diluter): test conducted under static conditions
- Initial cells density: 1.904 x 10E6 cells/mL
- Control end cells density: 10,000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 8 (test medium only)
GROWTH MEDIUM
- Standard medium used: yes according to guideline
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: culture medium prepared according to guideline
- Culture medium different from test medium: medium of the pre-culture and test culture were the same
- Intervals of water quality measurement: the pH of the test solutions was measured at the start and at the end of the exposure; temperature and light intensity were measured daily.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous light
- Light intensity and quality: ranged from 93.94 to 96.96 µE m-2 s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]: electronic particle counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.16
- Range finding study: performed
- Test concentration (range finding study): 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes
- Due to the very low water solubility of the test item individual Water Accommodated Fractions (WAFs) of the test item will be prepared according to the OECD guidance No. 23. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 63.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.33 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 54.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 11.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- The sensitivity of the test organism is routinely checked using 3,5-dichlorophenol as primary standard following internal SOPs in a non-GLP test twice a year. The latest (December 2017) ErC50 value of 2.90 mg/L (nominal; 95% confidence limits: 2.66 – 3.16 mg/L) for inhibition of growth rate is in good agreement with the results of an international ring test with an ErC50 of 3.38 ± 1.30 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a study conducted according to OECD Guideline 201 using Raphidocelis subcapitata as test organism, the EyC50 (0 -72 h) value was 54.9 mg/L and the LOEC and NOEC values were 20.0 and 6.33 mg/L, respectively, after 72 h of exposure. For the parameter growth rate the ErC50 (0 -72 h) value was > 200 mg/L and the LOEC and NOEC values were 63.3 and 20 mg/L, respectively, after 72 h of exposure.
- Executive summary:
In an alga growth inhibition test conducted according to OECD Guideline 201 (Alga, Growth Inhibition Test) cultures of Raphidocelis subcapitata were exposed to nominal test substance concentrations of 2.00, 6.33, 20.0, 63.3, and 200 mg/L Neodecanoic acid, tin(2+) salt (2:1) for 72 h under static conditions. The test substance concentrations were checked by DOC analysis at 0 and 72 h after application. For the parameter yield the EyC50 (0 -72 h) value was 54.9 mg/L and the LOEC and NOEC values were 20.0 and 6.33 mg/L, respectively, after 72 h of exposure. For the parameter growth rate the ErC50 (0 -72 h) value was > 200 mg/L and the LOEC and NOEC values were 63.3 and 20 mg/L, respectively, after 72 h of exposure.
The study is regarded as reliable without restriction and satisfies the requirements of the guideline.
Synopsis
Test organism: Raphidocelis subcapitata
Test type: static
Test duration: 72 h
Results:
Yield
EyC50 (0 -72 h): 54.9 mg/L
LOEC (0 -72 h): 20.0 mg/L
NOEC (0 -72 h): 6.33 mg/L
Growth rate
ErC50 (0 -72 h): > 200 mg/L
LOEC (0 -72 h): 63.3 mg/L
NOEC (0 -72 h): 20 mg/L (all values refer to nominal concentrations)
Endpoint(s) affected: growth rate, yield
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