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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March 12, 2018 - April 12, 2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Qualifier:
according to guideline
Guideline:
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
Activated sludge was collected from the Easton Wastewater Treatment Facility, Easton, Maryland on March 12, 2018. The Easton facility treats predominantly residential wastes. The sludge was sieved using a 2-mm screen and adjusted to approximately 1000 mg total suspended solids/L with mineral media and then aerated at test temperature until use. A total suspended solids measurement and standard plate count were performed on the inoculum on the day of use in the test. Plates were incubated at 20 ± 3ºC for approximately 48 hours.
Duration of test (contact time):
28 d
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (CO2 evolution)
Value:
95.4
Sampling time:
28 d
Parameter:
% degradation (CO2 evolution)
Value:
60
Sampling time:
11 d
Details on results:
Average Final pH 7.49

On days 2, 5, 8, 11, 15, 19, 22 and 26, the CO2 trap nearest the test chamber was collected for analysis of inorganic carbon. The two remaining traps were placed one position closer to the test chamber and a new trap was placed on the end of the series.
Results with reference substance:
The degradation of the reference substance was an average of 97.4% by the end of the test.
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
2-(2-MethoxyEthoxy) Ethyl Methacrylate was readily biodegradable in a Carbon Dioxide Evolution Test. The final mean percent biodegradation was 95.4 % TCO2 at the end of the 28-day test period. The pass level of 10% TCO2 within 10 days for ready biodegradability was also achieved for 2-(2-MethoxyEthoxy) Ethyl Methacrylate.
Executive summary:

In accordance with  OECD TG 301B following GLP conditions, 2-(2-MethoxyEthoxy) Ethyl Methacrylate was readily biodegradable in a Carbon Dioxide Evolution Test. The final mean percent biodegradation was 95.4 % TCO2 at the end of the 28-day test period.  The pass level of 10% TCO2  within 10 days for ready biodegradability was also achieved for 2-(2-MethoxyEthoxy) Ethyl Methacrylate.

Description of key information

2-(2-MethoxyEthoxy) Ethyl Methacrylate was shown to be readily biodegradable in a Carbon Dioxide Evolution Test. The final mean percent biodegradation was 95.4 % TCO2 at the end of the 28-day test period.  The pass level of 10% TCO2  within 10 days for ready biodegradability was also achieved for 2-(2-MethoxyEthoxy) Ethyl Methacrylate.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
freshwater

Additional information