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EC number: 247-368-0 | CAS number: 25956-17-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- 24 and 48 hrs
- Hardness:
- 150.5 mg of CaCO3
- Test temperature:
- 20±2
- pH:
- 7.1
- Nominal and measured concentrations:
- 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: No effects were observed
- Validity criteria fulfilled:
- yes
- Remarks:
- 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
- Conclusions:
- Based on nominal concentration, experimental median effective Concentrations [EC50 (48 h)] for test material on test daphnia was found to be >100 mg/L.
- Executive summary:
Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for determining toxicity of test material. The test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively.
The nominal concentration selected for the experiment was 100 mg/l and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/l.
Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, does not exhibit short term toxicity to Daphnia and can be considered to be not classified as per the CLP classification criteria.
Reference
9.1. Immobility
Nominal Concentration (mg/l) |
Number of Daphnids |
Immobility |
||
0 h |
24h |
48h |
||
Control |
10 |
- |
- |
- |
100 |
10 |
- |
- |
- |
pH and oxygen content:
Nominal Concentration (mg/l) |
Number of Daphnids |
pH(mg/L) |
|
|
0 h |
48h |
|
||
Control |
10 |
7.5 |
7.7 |
|
100 |
10 |
7.7 |
7.9 |
|
Nominal Concentration Number of |
DO(mg/L) |
|
||
(mg/l) |
|
daphnids |
0h |
48h |
Control |
|
10 |
7.0 |
6.8 |
100 |
|
10 |
7.0 |
6.7 |
Test temperature:
Nominal Concentration Number of |
Temparature |
||||
(mg/l) |
|
daphnids |
0h |
48h |
|
Control |
|
10 |
21.2 |
21.5 |
|
100 |
|
10 |
21.2 |
21.5 |
Description of key information
Based on nominal concentration, experimental median effective Concentrations [EC50 (48 h)] for test material on test daphnia was found to be >100 mg/L. Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, does not exhibit short term toxicity to Daphniaand can be considered to be not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Based on the experimental data for the target as well as read-across analogues which are extracted by using mechanistic approach and functionally and structurally similar to the target chemical, toxicity of test chemical were determined on the basis of immobility of daphnia magna. The studies are summarized as below:
Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for determining toxicity of test material. The test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively.
The nominal concentration selected for the experiment was 100 mg/l and test Daphnia magna were exposed to this concentration for 48 hours. The median lethal concentration (EC50) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/l.
Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, does not exhibit short term toxicity to Daphnia and can be considered to be not classified as per the CLP classification criteria.
Objective of second study was to determine the effect of test chemical on the mobility of daphnia magna. Test was conducted in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). A limit test at sample concentration of 100 mg/L was performed. Effects on immobilisation were observed for 48 hours. The solution 100.0 mg/L was prepared by dissolving dark blue powder in reconstituted water. The solution was kept 5 min in ultrasonic bath. The median effective concentration (EC50) for the test substance on water flea, Daphnia magna was determined to be >100 mg/L on the basis of mobiity inhibition effects in a 48 hour study as EC50 was observed at the limit test concentration 100 mg/l. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance does not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna) and thus not classified as per the CLP classification criteria.
Again to support both the studies, another study was performed to determine the toxicity of test chemical on D. magna for 48 hours in a static system. Daphnids were exposed to test chemical in 50 ml beakers in a volume of 25 ml of liquid solution containing both the chemical and media as specified in OECD 202. The nominal concentrations used were 100 mg/L (limit test).
The IC50 was defined as a concentration that immobilizes 50% of the exposed D. magna. Eight percent immobilization in D. magna was observed after 48 hours of exposure to 100 mg/L of test chemical. The EC50 was therefore estimated to be >100 mg/L.
Based on the EC50 value, it can be concluded that the chemical was nontoxic and was considered to be not classified as per CLP classification criteria.
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