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EC number: 239-044-2 | CAS number: 14970-87-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06-13-16 to 09-20-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Aliquots were removed from each prepared concentration every day on fresh and old solutions for the definitive test.
Analytical analysis was performed immediately after sampling. - Vehicle:
- no
- Details on test solutions:
- Test solutions were prepared from dilutions of a stock solution.
439 µL of test item was poured into a 5L volumetric flask, the volume was then made up to 5000 mL with test medium.
The solution was kept under high speed stirring at ambient temperature during approximately 10 minutes with a magnetic stir bar.
No undissolved particles were observed, this solution was thus not filtered before dilution.
The pH of the test solutions were not adjusted.
Test solutions were renewed everyday throughout the 96h test period (semi-static test). - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- The test organisms were Danio rerio (Teleostei, Cyprinidae), obtained on the 28 July 2016 from a recognised supplier: La Grande Rivière France (69490 Saint-Forgeux).
All fish were in good health and free from any apparent malformation.
Mortality of the batch was less than 5% in the week preceding the start of the study.
No disease treatments were administered throughout holding and testing.
Holding was maintained within the laboratory at a temperature of 21-25°C in glass tanks.
A light cycle of 12 h light and 12 h dark was applied, illumination being provided by fluorescent tubes (intensity between 400 and 800 lux at the surface of the tanks).
Tanks were aerated to ensure that the dissolved oxygen concentration is at least 60% of air saturation value in holding tanks and in test tanks.
During holding, fish were fed twice per day with ground flake food TetraMin®.
The fish were not fed for a period of 24h prior to test commencement and throughout the duration of the test
Fish were added to the test tanks within 30 min of the completion of preparation of the test solutions.
All test fish were weighed prior to the test and a representative number of test fish batch (10 at random) was measured after the test to assess compliance with guideline criteria. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 22.7 °C
- pH:
- 7.5 - 8.0
- Dissolved oxygen:
- 79.0 - 102.3 % saturation
- Salinity:
- Freshwater.
- Conductivity:
- Not measured.
- Nominal and measured concentrations:
- Nominal concentrations:
0 ; 0.9 ; 2.1 ; 4.5 ; 10 ; 22 mg/L
Geometric mean concentrations:
0 ; 0.09 ; 0.16 ; 0.23 ; 5.7 ; 16.1 mg/L - Details on test conditions:
- - Test tanks (filled with 2.5 L of test solution) containing fish were incubated with a photo period of 9 hours of darkness per 24 h
- All test tanks were made of clear glass and covered by plates with ventilation holes to minimise evaporation and avoid the entry of dust into the solutions.
- Test solutions were prepared using the synthetic medium.
- Chemical reagents used for the preparation of the dilution water was were of “analytical grade”.
- The control treatment was maintained under the same conditions, with no test item. - Reference substance (positive control):
- yes
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 5.7 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- Mortalities are presented in table 1.
No mortality were observed in the control.
No abnormal behaviour was observed.
Calculated 96h-LC50 is 6.2 mg/L. - Results with reference substance (positive control):
- The sensitivity of the test system and the methodology are evaluated with every batch of fish by performing an acute toxicity test on potassium dichromate. The values of 24h-LC50 obtained with the batch used was as follows: Batch 16/Br/01/2: 24h-LC50 = 173.6 mg/L (obtained on 11 August 2016). This result is consistent with the laboratory control quality chart.
- Reported statistics and error estimates:
- Raw data were treated according to the recommendations of the guideline. All statistical analysis were conducted using the Software ToxRatPro Version 3.1.0.
As there was evidence that the concentration of the substance being tested were not satisfactorily maintained within ± 20% of the nominal or measured initial concentration throughout the test, analysis of the results was based on geometric mean concentration during exposure. - Sublethal observations / clinical signs:
Table 1:
Test solution
(mg/L)
Cumulative dead fish
Mortality at the end of the period
T0
T24h
T48h
T72h
T96h
Control
0
0
0
0
0
0.0%
0.9
0
0
0
0
0
0.0%
2.1
0
0
0
1
1
14.3%
4.5
0
0
0
0
0
0.0%
10.0
0
0
0
1
1
14.3%
22.0
0
2
6
7
7
100.0%
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test item to Danio rerio exposed for 96h was assessed according to the OECD 203 Guideline. Validity criteria were fulfilled.
An important loss of the test item was observed. As a consequence the exposure concentrations were based on the geometric mean of measured concentrations. On this basis, 96h-LC50 was 5.7 mg/L - Executive summary:
This study was designed to determine the effects of the test item to Danio rerio over a 96h period with static conditions (Range–finding test) then semi-static test (Definitive test), according to the OECD 203 Guideline. The test solutions (including control) were prepared from dilutions of a stock solution and renewed daily throughout the test (semi-static test).
Chemical analysis of the test item throughout the test period have shown unstability of the substance. That's why geometric means of measured concentrations were used to determine 96h-LC50 = 5.7 mg/L
Reference
Description of key information
This study was designed to determine the effects of the test item toDanio rerioover a 96h period with static conditions (Range–finding test) then semi-static test (Definitive test), according to the OECD 203 Guideline. The test solutions (including control) were prepared from dilutions of a stock solution and renewed daily throughout the test (semi-static test).
Chemical analysis of the test item throughout the test period have shown unstability of the substance. That's why geometric means of measured concentrations were used to determine 96h-LC50 = 5.7 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 5.7 mg/L
Marine water fish
Marine water fish
- Effect concentration:
- 0.5 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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