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EC number: 228-906-3 | CAS number: 6372-81-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- According to OECD and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Barium bis[2-chloro-5-[(2-hydroxy-1-naphthyl)azo]toluene-4-sulphonate]
- EC Number:
- 225-935-3
- EC Name:
- Barium bis[2-chloro-5-[(2-hydroxy-1-naphthyl)azo]toluene-4-sulphonate]
- Cas Number:
- 5160-02-1
- Molecular formula:
- C17H13ClN2O4S.1/2Ba
- IUPAC Name:
- barium bis{5-chloro-2-[(2-hydroxy-1-naphthyl)diazenyl]-4-methylbenzenesulfonate}
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- In order to determine the substance concentration of the test solution, samples were taken at study start (0 h) and study end (48 h); representative sample of total water amount 400 mL: 20 mL
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):
A saturated solution was tested in a limit test.
1 g of the test substance was weighed into a graduated 1000 mL flask, filled up with test water and stirred for about 24 h with a magnetic stirrer. The subspension was filtrated through a fluted filter. The filtrate, a clear light red solution, was used as test solution.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna - Clone 5 (IRChA, France)
- Source: stock culture of the Department of Toxicology, Hoechst AG
- Method of breeding:
Culture: In 100 mL beakers filled with 70 mL culture medium, 1 daphnid per culture vessel up to an age of 42 days; the reproduction rate and the state of health were monitored daily apart from weekends.
Culture medium: demineralized water with a conductance of < 1 µS/cm according to Elendt
- Feeding during test: no feeding during the study
ACCLIMATION
- Acclimation period: not necessary; identical breeding and test conditions
- Type of food: monocellular green algae (Scenedesmus subspicatus)
- Feeding frequency: 3 times per week ad libitum
Reference:
Elendt, B. P. (1990): Selenium Deficiency in Crustacea, Protoplasma, 154, 25-30
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 80.1 Ca, 12.2 Mg, 144.9 HCO3 [mg/L]
- Test temperature:
- 20.8 - 21.0 (concentration group)
20.2 - 21.0 (control) - pH:
- 7.6 - 8.3 (concentration group)
8.0 - 8.3 (control) - Dissolved oxygen:
- 8.3 - 8.7 (concentration group)
9.0 (control) - Nominal and measured concentrations:
- measured concentrations:
control: < 0.1 mg/L (0 h and 48 h)
concentration group: 5.14 mg/L at 0 h; 2.39 mg/L at 48 h - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass beakers, height 110 mm, diameter 70 mm; 200 mL test water
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demineralized water with a conductance of < 1 µS/cm according to Elendt
OTHER TEST CONDITIONS
- Photoperiod: 12/12 h light/dark cycle
- Light intensity: illumination by fluorescent tubes diffuse light, illumination range max. 20 μE⋅m-2 ⋅s-1.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation at 24 and 48 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Limit test with saturated solution (see above)
- Justification for using less concentrations than requested by guideline: limit test - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 3.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 3.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No immobilisation after 24 and 48 hours observed with the test substance (saturated solution) or the control.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- At the saturated solution (3.8 mg/L (mean)) of the test item no biologically significant effect was determined.
- Executive summary:
In the acute immobilisation test with Daphnia magna the effect of the saturated solution (3.8 mg/L) of C.I. Pigment Red 53:1, was determined according to OECD 202 and Directive 79/831/EEC. The limit test was conducted under static conditions over 48 h. Water quality parameters pH-value and dissolved oxygen concentration, measured at 0 and 48 h, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.
At the saturated solution of the test item, 3.8 mg/L (mean), no biologically significant effect was determined.
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