Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 226-009-1 | CAS number: 5216-25-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
EC50 > 100 mg/l, nominal concentration
Key value for chemical safety assessment
Additional information
Two studies on short-term toxicity to aquatic invertebrates are available.
In the key study, the acute toxicity of the test item to Daphnia magna was determined in a 48‑hour static test according to the OECD Guideline 202 (2004). No analitical monitoring was however performed during test.
For this purpose, juvenile daphnids (< 24 hours old at initiation of the test) were exposed to an aqueous test medium containing the test item at the only nominal concentration of 100.0 mg/L. The exposed organisms were checked for immobilization 24 and 48 hours after test initiation.
The pH of the control (test medium without test item) was 7.13 and the pH of test item solution immediately after preparation was 7.03, therefore, the pH of the test medium was not adjusted before the start of the test.
The room temperature was in the range 21.2 °C during the test period, according to the OECD’s recommended range (20 ± 2°C).
Light intensity during the 48 hours of test period was in the range 368-401 Lux, within the range 200-1500 Lux.
At the end of test period, in the negative control no immobilization was observed and no daphnid was found trapped on the test water surface. These values comply with the specified validity criterion that immobilization in the negative control group should not exceed 10% at the end of the test.
No immobilization was observed in the test item medium after 24 and 48 hours, therefore the NOEC (highest concentration tested without toxic effects after the exposure period) was determined to be equal to the nominal tested concentration of 100.0 mg/L.
Based on the observed effects, the LOEC and the 48-h IC50were determined to be greater than the tested concentration, 100.0 mg/L.
In the supporting study, the authors assessed the acute toxicity of p-chlorobenzotrichloride to Daphnia magna Straus (Crustacea) in a static system. The test was performed according to DIN 38 412 Part 11, which is similar to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The test organisms were exposed for 24h to six different concentrations of p-chlorobenzotrichloride (i.e. 8.31; 83.1; 103.9; 166.3; 415.7; 831.4 mg/L) and a negative as well as a reference control (i.e. potassium dichromate; 2.0 mg/l) was included. For each condition, two replicates, each containing 10 dapnids between 3 and 24h, old were used.
Under the test conditions an EC50 (24h) was reported of 209.9 mg/L with a 95% confidence interval ranging from 188.4 to 417.4 mg/L and the negative and reference control gave the expected results.
Although the statistical analysis used results in a EC50-value with a large 95% confidence interval, it is likely that performing the test according to the OECD guideline 202 (meaning 48h of exposure and analysis with appropriate statistical methods such as probit analysis) would also give a EC50-value above 100 mg/L but less than this observed value.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.