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EC number: 225-713-6 | CAS number: 5025-82-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- All solutions were sterilized before use.
Composition of the Solutions:
6.4.1 Stock Solution I
NH4Cl 1500 mg
MgCl2*6H2O 1200 mg
CaCl2*2H2O 1800 mg
MgSO4*7H2O 1500 mg
KH2PO4 160 mg
H2O deionised ad 1000 mL
6.4.2 Stock Solution II
FeCl3*6H2O 64 mg
Na2EDTA*2H2O 100 mg
H2O deionised ad 1000 mL
6.4.3 Stock Solution III
H3BO3 185 mg
MnCl2*4H2O 415 mg
ZnCl2 3 mg
CoCl2*6H2O 1.5 mg
CuCl2*2H2O 0.01 mg
Na2MoO4*2H2O 7 mg
H2O deionised ad 1000 mL
6.4.4 Stock Solution IV
NaHCO3 50 g
H2O deionised ad 1000 mL
6.4.5 Algal medium (OECD TG 201)
Stock solution I 10.0 mL
Stock solution II 1.0 mL
Stock solution III 1.0 mL
Stock solution IV 1.0 mL
H2O deionised ad 1000 mL - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Unicellular freshwater green alga.
Genus Desmodesmus
Species subspicatus
SAG Strain Number 86.81
Taxonomic position Chlorophyta - Chlorophyceae - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21.5 – 22.5 °C
- Details on test conditions:
- Duration: 72 hours
Treatments: 100 / 46 / 22 / 10 / 4.6 mg/L (nominal)
The concentrations to be tested are based on a non GLP pre-test.
Temperature: 21.5 – 22.5 °C
Lighting: within the specified range (4440 – 8880 Lux)
Measurement: cell number via electronic cell counter (Measurement range: 5 – 12 µm)
Replicates
Treatments: 3 vessels, each filled with 45 ±1 mL test solution and algae
1 vessel, filled with 45 ±1 mL test solution without algae for analytical determination
Blank Control: 6 vessels, each filled with 45 ±1 mL algal medium and al-gae
1 vessel filled with 45 ±1 mL algal medium without algae for analytical determination - Reference substance (positive control):
- yes
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 78.43 mg/L
- 95% CI:
- > 40.98 - < 150.11
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L (nominal). Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concen-tration of each replicate was determined by measuring the cell numbers every 24 ± 1 hours with an electronic cell counter. Growth rate µ and the yield were determined from the cell number at the respective observation times.
Slight but statistically significant inhibition of algal growth was observed only at the highest tested concentration of 100 mg/L.
At the start and at the end of the test, the content of the test item in the test solutions was determined using HPLC.
The measured concentrations lay between 101 % and 108 % of the nominal concentrations at the beginning of the test and between 104 % and 117 % of the nominal concentrations at the end of the test. Therefore, the determination of the results was based on the nominal concentration (see Guidance Doc. No.23 (§176)).
Due to the low inhibition values, the EC50 was not determined by statistical evaluations, but is stated as higher than the highest tested concentration of 100 mg/L.
For the growth rate, a value for EC10 of 141.16 mg/L, was extrapolated by the program which was far above the highest concentration tested. The EC10 is therefore also given as higher than 100 mg/L.
The 72h-EC50 values of potassium dichromate were determined in a separate reference test. The values were within the range of the laboratory.
The pH of the blank control should not fluctuate by more than 1.5 units. The change was 0.6 units in the blank control.
All validity criteria were met.
No observations were made which might cause doubts concerning the validity of the study outcome. The result of the test can be considered valid. - Executive summary:
One valid experiment was performed.
The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L (nominal). Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concen-tration of each replicate was determined by measuring the cell numbers every 24 ± 1 hours with an electronic cell counter. Growth rate µ and the yield were determined from the cell number at the respective observation times.
Slight but statistically significant inhibition of algal growth was observed only at the highest concentration of 100 mg/L.
At the start and at the end of the test, the content of the test item in the test solutions was determined using HPLC.
The measured concentrations lay between 101 % and 108 % of the nominal concentrations at the beginning of the test and between 104 % and 117 % of the nominal concentrations at the end of the test. Therefore, the determination of the results was based on the geometric mean of the measured concentrations (see OECD 201 (§ 39)).
Therefore, the determination of the biological results was based on the nominal concentra-tion (see Guidance Doc. No.23 (§176)).
The 72h-EC50 values of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were deter-mined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.64 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).The following results for the test item 3-acetylthiazolidine-4-carboxylic acid were determined:
Table 3 Results of the test item
Endpoint NOEC LOEC EC10 EC50
Growth Rate 46.00 mg/L 100.00 mg/L >100.00 mg/L >100.00 mg/L
Yield 46.00 mg/L 100.00 mg/L 78.43 mg/L >100.00 mg/LNote: The NOEC is determined by comparing each treatment with the blank control using a hypothesis test. A statistically insignificant variation is considered as "no observed effect". In contrast, EC10 is determined by regression analysis and read from the toxicity vs. con-centration graph. Due to the low inhibition values, the EC50 was not determined by statisti-cal evaluations, but is stated as higher than the highest tested concentration of 100 mg/L.
For the growth rate, a value for EC10 of 141.16 mg/L, was extrapolated by the program which was far above the highest concentration tested. The EC10 is therefore also given as higher than 100 mg/L.
Reference
Calculation of results was performed with the help of a validated software (Microsoft Ex-cel®). The estimation of the biological data was accomplished using the software ToxRat® Professional, version 3.3.0. The details of calculation are stated in Annex 5: Statistical cal-culation using ToxRat® Professional 3.3.0.
The following values were determined.
Table 10 Biological Results of the Test Item
Parameter | Value | 95 % confidence interval |
NOEC (Growth Rate) 72 h | 46.00 mg/L | -- |
NOEC (Yield) 72 h | 46.00 mg/L | -- |
LOEC (Growth Rate) 72 h | 100.00 mg/L | -- |
LOEC (Yield) 72 h | 100.00 mg/L | -- |
72h ErC10 | > 100.00 mg/L* | -- |
72h EyC10 | 78.43 mg/L | 40.98 – 150.11 mg/L |
72h ErC50 | > 100.00 mg/L | -- |
72h EyC50 | > 100.00 mg/L | -- |
Due to the low inhibition values, the EC50 was not determined by statistical evaluations, but is stated as higher than the highest tested concentration of 100 mg/L
For the endpoint growth rate, an EC10 of 141.16 mg/L was extrapolated by the program. This value lies far above the highest tested concentration 100 mg/L Therefore for the end-point growth rate the EC10 was reported as the range > 100 mg/L.
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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