1,2-dichloro-3-nitrobenzene

Endpoint summary

Description of key information

The skin irritation potential of 1,2-dichloro-3-nitrobenzene was evaluated according to the OECD guideline 404. No signs for skin irritation were observed throughout the test and observation period.

The eye irritation potential of 1,2-dichloro-3-nitrobenzene was investigated in 3 New Zealand White rabbits compliant with the OECD guideline 405. 1,2-dichloro-3 -nitrobenzene induced slight conjunctival redness and swelling, as well as moderate efflux 1 h after administration. These effects were fully reversible within 24 h. The mean scores over 24, 48 and 72 h were 0.0 for corneal opacity and iritis in all animals.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study.

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Version / remarks:

adopted in 1981

Deviations:

yes

Remarks:

8 d observation period

GLP compliance:

not specified

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Lippische Versuchstierzucht, Hagemann GmbH & Co. KG, Extertal, Germany
- Weight at study initiation: 2.7 kg
- Housing: single
- Diet (e.g. ad libitum): mümmel z (ssniff, Soest, Germany) ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 7 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16-18
- Humidity (%): 40
- Photoperiod (hrs dark / hrs light): 12/12

Type of coverage:

semiocclusive

Preparation of test site:

other: clipped skin

Vehicle:

other: oil (not further specified)

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 500 mg

Duration of treatment / exposure:

4 h

Observation period:

1, 24, 48 and 72 h, and 8 days

Number of animals:

3

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: score = 0 at any time point

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: score = 0 at any time point

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24 h

Score:

0

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 48 h

Score:

0

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 72 h

Score:

0

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 h

Score:

0

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 48 h

Score:

0

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 72 h

Score:

0

Erythema/edema scores

	Time point after patch removal	Animal No.			Sum	Average
		1	2	3
Erythema score	1 h	0	0	0
	24 h	0	0	0	0	0
	48 h	0	0	0	0	0
	72 h	0	0	0	0	0
	8 days	0	0	0
Edema score	1 h	0	0	0
	24 h	0	0	0	0	0
	48 h	0	0	0	0	0
	72 h	0	0	0	0	0
	8 days	0	0	0

Interpretation of results:

GHS criteria not met

Executive summary:

Schreiber G (1982)

The skin irritation potential of 1,2-dichloro-3-nitrobenzene was evaluated according to the OECD guideline 404 (adopted: 12 May 1981). 0.5 g 1,2 -dichloro-3 -nitrobenzene ground in oil was applied to the clipped left flank of each of 3 New Zealand White rabbits for 4 h under semi-occlusive conditions. Thereafter patches and test material were removed. The test sites were examined and scored for erythema and edema using the Draize method 1, 24, 48 and 72 h as well as 8 days after exposure. The mean erythema and edema scores over 24, 48 and 72 h were 0.0 for both end points in all animals. No signs for skin irritation were observed throughout the test and observation period.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Version / remarks:

adopted in 1981

Deviations:

yes

Remarks:

observation period 8 d

GLP compliance:

not specified

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Lippische Versuchstierzucht, Hagemann GmbH & Co. KG, Extertal, Germany
- Weight at study initiation: 2.7 kg
- Housing: single
- Diet (e.g. ad libitum): mümmel z (ssniff, Soest, Germany) ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 7 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16-18
- Humidity (%): 40
- Photoperiod (hrs dark / hrs light): 12/12

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100 µl bulk volume

Duration of treatment / exposure:

continuous

Observation period (in vivo):

1, 24, 48, and 72 h, and 8 days

Number of animals or in vitro replicates:

3

Details on study design:

TOOL USED TO ASSESS SCORE: fluorescein

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: score = 0 at 24, 48 and 72 hrs

Irritation parameter:

iris score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

2

Reversibility:

other: score = 0 at 24, 48 and 72 hrs

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

3

Reversibility:

other: score = 0 at 24, 48 and 72 hrs

Irritation parameter:

chemosis score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: score = 0 at 24, 48 and 72 hrs

Irritation parameter:

conjunctivae score

Remarks:

redness

Basis:

mean

Time point:

other: 1 h

Score:

1

Max. score:

3

Reversibility:

fully reversible within: 24 h

Remarks on result:

other: fully reversible

Irritation parameter:

conjunctivae score

Remarks:

swelling

Basis:

mean

Time point:

other: 1 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 24 h

Remarks on result:

other: fully reversible

Irritation parameter:

conjunctivae score

Remarks:

efflux

Basis:

mean

Time point:

other: 1 h

Score:

2

Max. score:

3

Reversibility:

fully reversible within: 24 h

Remarks on result:

other: fully reversible

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 1, 24, 48 and 72 h

Score:

0

Max. score:

4

Reversibility:

other: no effects

Remarks on result:

other: no effects

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 1, 24, 48 and 72 h

Score:

0

Max. score:

2

Reversibility:

other: no effects

Remarks on result:

other: no effects

No signs of eye irritation according to cornea and iris scores were observed throughout the test and observation period. One hour after application of the test substance, all animals showed slight conjunctival redness and swelling, as well as moderate efflux. These effects were fully reversible within 24 h.

Executive summary:

The eye irritation potential of 1,2-dichloro-3-nitrobenzene was investigated in 3 New Zealand White rabbits compliant with the OECD guideline 405 (adopted: 12 May 1981). A bulk volume of 0.1 mL of the crystalline test substance was applied into the conjunctival sac of the right eye of each animal, the left eye serving as control. The eyes were not rinsed after administration of the test substance. The eye irritation reactions were examined and scored using the Draize method 1, 24, 48 and 72 h as well as 8 days after application. 1,2-dichloro-3-nitrobenzene induced slight conjunctival redness and swelling, as well as moderate efflux 1 h after administration. These effects were fully reversible within 24 h. The mean scores over 24, 48 and 72 h were 0.0 for corneal opacity and iritis in all animals (Schreiber, 1982).

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Additional information

The skin irritation potential of 1,2-dichloro-3-nitrobenzene was evaluated according to the OECD guideline 404 (adopted: 12 May 1981). 0.5 g 1,2 -dichloro-3-nitrobenzene ground in oil was applied to the clipped left flank of each of 3 New Zealand White rabbits for 4 h under semi-occlusive conditions. Thereafter patches and test material were removed. The test sites were examined and scored for erythema and edema using the Draize method 1, 24, 48 and 72 h as well as 8 days after exposure. The mean erythema and edema scores over 24, 48 and 72 h were 0.0 for both end points in all animals. No signs for skin irritation were observed throughout the test and observation period (Schreiber 1982).

The eye irritation potential of 1,2 -dichloro-3-nitrobenzene was investigated in 3 New Zealand White rabbits compliant with the OECD guideline 405 (adopted: 12 May 1981). A bulk volume of 0.1 mL of the crystalline test substance was applied into the conjunctival sac of the right eye of each animal, the left eye serving as control. The eyes were not rinsed after administration of the test substance. The eye irritation reactions were examined and scored using the Draize method 1, 24, 48 and 72 h as well as 8 days after application. 1,2-dichloro-3-nitrobenzene induced slight conjunctival redness and swelling, as well as moderate efflux 1 h after administration. These effects were fully reversible within 24 h. The mean scores over 24, 48 and 72 h were 0.0 for corneal opacity and iritis in all animals (Schreiber 1982).

Justification for classification or non-classification

1,2-dichloro-3-nitrobenzene is not irritatin to skin and eyes. According to CLP classification criteria (Regulation (EC) No 1272/2008) a classification is not justified.