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EC number: 217-210-5 | CAS number: 1777-82-8
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The ready biodegradability of the test substance was examined in a test (BASF SE, 01/0492/26/2, 2003) according to OECD 301 F (Manometric Respirometry Test, 1992) under GLP conditions. The test substance was degraded by an average of < 10 % (mean value) after 28 days. The test substance was found to be not readily-biodegradable under the test conditions and the 10 days windows criterion was consequently not fulfilled.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The ready biodegradability of the test substance was examined in a test (BASF SE, 01/0492/26/2, 2003) according to OECD 301 F (Manometric Respirometry Test, 1992) under GLP conditions. The biodegradation of the test substance was followed by exposure to the activated sludge of a domestic waste water treatment plant from Mannheim (Germany). The sludge was washed and aerated for 24 hours and then added to the test vessels to obtain a sludge concentration of 30 mg/L dry substance. Mixtures of the test substance in a concentration of 100 – 101 mg/L (corresponding to a ThOD = 1349 mg/g), a defined inorganic medium and an inoculum, which was not pre-adapted, were incubated and aerated in a respirometer (Sapromat) at 22 ± 1°C up to 28 days. The substance specific analysis by gas-chromatography at the end of the test showed a removal of 30 % (mean value) in the vessels with inoculum and no removal in the vessel with deionized water only (for abiotic elimination). The chromatograms gave no indication of the formation of metabolites. The measured concentrations of the test substance were determined to be in a range of 25.0 to 25.3 mg/L. As a reference compound (procedure control), aniline was tested simultaneously under the same conditions. In the procedure controls, the reference compound aniline was readily biodegraded by an average of 70 - 80 % after 14 days of exposure. The validity criteria were met. The test substance was degraded by an average of < 10 % (mean value) after 28 days. The test substance was found to be not readily-biodegradable under the test conditions and the 10 days windows criterion was consequently not fulfilled.
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