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EC number: 213-497-6 | CAS number: 959-26-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental Starting Date 16. Aug. 2021
Experimental Completion Date 22. Sep. 2021 - Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Version / remarks:
- 2008
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name: BHET
Batch no.: AWS0274J
Purity: ~95% - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Activation basin of the sewage treatment plant, In den Seewiesen, 67482 Edenkoben
- Pretreatment: The sludge was filtrated through a cloth, washed with test medium (2x) and resuspended in test medium. It was then aerated until use.
- Concentration of sludge: The dry matter was determined to contain 3.96 g of suspended solids/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- IC (inorganic carbon)
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Composition of medium:
- Solution a: Potassium dihydrogen phosphate, Dipotassium hydrogen phosphate, Disodiumhydrogen phosphate dihydrate, Ammonium chloride, Water
- Solution b: Calcium chloride dihydrate, Water
- Solution c: Magnesium sulphate heptahydrate, Water
- Solution d: Iron(III) chloride hexahydrate, Di-sodium-ethylene diaminetetraacetate dihydrate, Water
- Test Medium: Freshly prepared. 10 mL of solution a were mixed with 800 mL water, then 1 mL of solutions b, c and d were added and filled up to 1 L with water
- Test temperature: 19.7 – 21.9 °C without direct lighting
- Inoculum concentration: 25.0 mg/L
TEST SYSTEM
The test vessels were aerated with purified, CO₂-scrubbed, moistened air. The scrubbing of carbon dioxide was achieved by bubbling the purified air through a flask containing 1.5 M NaOH. To control the absence of CO₂, the air was then led through a flask containing a solution of Ba(OH)₂ before reaching the test vessels.
Magnetic stirrers were used to prevent deposition of inoculum.
The emitted CO₂ was trapped in 0.25 M NaOH. Two scrubbers containing 100 mL each were connected in series to the test vessels.
Apparatus blanks: 2, containing mineral medium only
Blank controls: 2, containing mineral medium and inoculum
Positive control flasks: 2, containing positive control, mineral medium and inoculum
Test flasks: 2, containing test item, mineral medium and inoculum
Abiotic control: 1, containing test item, mineral medium and HgCl₂
Toxicity control: 1, containing test item, positive control, mineral medium and inoculum
SAMPLING
From each front scrubber flask, 9 samples were taken in order to determine the emitted CO₂ (on day 2, 4, 7, 9, 11, 14, 18, 23 and 29). The sample volume was 1 mL. The resulting change in the volume of the front flask was considered in the calculation of emitted CO₂.
On day 0, only one sample of 0,25 M NaOH was sampled. This measured value was used as start value for all treatments.
On day 28, 5 mL HCl₂ M was added to each test flask in order to drive off dissolved CO₂. On day 29, samples from both scrubber flasks were taken. - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 87
- Sampling time:
- 28 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 82
- Sampling time:
- 14 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 35.1
- Sampling time:
- 4 d
- Details on results:
- Degradation behaviour of positive control and toxicity control was normal.
Abiotic degradation was not observed.
The test item is not toxic towards the inoculum in a concentration of 38.4 mg/L.
Validity criteria
Inorganic carbon content of test item solution in medium: < 5% of total carbon - valid (0%)
CO₂ emitted by the controls: < 70 mg/L - valid (10.9 mg/L)
Difference within replicates: <20% - valid (2.3%)
Degradation of positive control ≥ 60%: ≤ 14 days - valid (7 days)
Degradation in the toxicity flask on day 14: > 25% - non-toxic (85.5%) - Results with reference substance:
- 83.6% degradation of aniline within 29-days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item is readily biodegradable; 87% in 28-days
- Executive summary:
The test item was tested using a concentration of nominally 20 mg organic carbon/L in test medium following OECD 301B and EU-Method C.4-C and according to GLP.
Aniline was chosen as positive control. Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L). The test was left running for 28 days. All validity criteria were met. Degradation of the positive control surpassed the pass level of 60 % after 7 days.
The following data were determined for the test item:
10-day-window: day 3 – 13
degradation at the end of 10-day-window: 80 %
degradation at the end of the test: 87 %
Therefore, when applying the 10-day-window, the test item is readily biodegradable following OECD 301B and EU C.4-C respectively.
Reference
Description of key information
The test item is readily biodegradable; 85% in 28-days.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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