2-methylanisole

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Acute Immobilization Study of 2-Methylanisole (578-58-5) on Daphnia magna in a Semi-Static System for 48 hrs. Test performed according to the OECD guideline 202.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

The test substance 2-Methylanisole (578-58-5) was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

24 and 48 hrs

Hardness:

150.5 mg of CaCO3

Test temperature:

20±2

pH:

7.1

Nominal and measured concentrations:

6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Immobility

Nominal Concentration (mg/l)	Number of Daphnids	Immobility
		0 h	24 h	28 h
Control	10	-	-	-
6.25	10	-	-	-
12.5	10	-	-	-
25	10	-	-	-
50	10	-	-	-
100	10	-	-	-

 

 

pH and oxygen content:

Nominal Concentration (mg/l) Number of Daphnids Immobility 0 h 28 h Control 10 7.4 7.5 6.25 10 7.5 7.6 12.5 10 7.5 7.7 25 10 7.6 7.8 50 10 7.6 7.8 100 10 7.6 7.8

 

Nominal Concentration (mg/l)	Number of Daphnids	DO(mg/L)
		0 h	28 h
Control	10	7.0	6.8
6.25	10	7.0	6.8
12.5	10	7.0	6.8
25	10	7.0	6.8
50	10	7.0	6.8
100	10	7.0	6.8

Test temperature:

Nominal Concentration (mg/l)	Number of Daphnids	Temparature
		0 h	28 h
Control	10	21.5	21.9
6.25	10	21.5	21.9
12.5	10	21.5	21.9
25	10	21.5	21.9
50	10	21.5	21.9
100	10	21.5	21.9

Validity criteria fulfilled:

yes

Remarks:

1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels

Conclusions:

Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for 2-Methylanisole (578-58-5) on test daphnia was found to be > 100 mg/L.

Executive summary:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for 2-Methylanisole (578-58-5). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively.The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for 2-Methylanisole (578-58-5) on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be > 100 mg/L. Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, 2-Methylanisole (578-58-5) does not exhibit short term toxicity to Daphnia. EC50 (48 hours) Experimental is > 100 mg/L.

After 48 hours of exposure to test item 2-Methylanisole (578-58-5) to various nominal test concentrations, EC50 was determine to be >100 mg/l . Based on the EC50, it can be consider that the chemical was non hazardous and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for 2-Methylanisole (578-58-5) on test daphnia was found to be > 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Based on the experimental study report,Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for 2-Methylanisole (578-58-5). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively.The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for 2-Methylanisole (578-58-5) on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be > 100 mg/L. Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, 2-Methylanisole (578-58-5) does not exhibit short term toxicity to Daphnia. EC50 (48 hours) Experimental is > 100 mg/L.

After 48 hours of exposure to test item2-Methylanisole (578-58-5)to various nominal test concentrations, EC50 was determine to be >100 mg/l . Based on the EC50, it can be consider that the chemical was non hazardous and can be consider to be not classified as per the CLP classification criteria.