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REACH

2-methylanisole

EC number: 209-426-3 | CAS number: 578-58-5

Life Cycle description
Uses advised against

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical 2-Methylanisole (578-58-5) on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

Analytical monitoring:

Vehicle:

Details on test solutions:

The test substance was partially soluble in water. Therefore, the stock solution will be prepared by dissolving 1 g of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous 4 hour stirring. After the completion of 4 hour stirring, stock solution was analytically detected and then test solution will be prepared for achieving the test concentration of 3.125 mg/L, 6.25 mg/L,12.5 mg/L, 25 mg/L and 50 mg/L, respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average ----- cm
- Weight at study initiation (mean and range, SD): average ------- g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: ----------
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

152.5 mg of CaCO3

Test temperature:

23.8 °C

pH:

7.4

Dissolved oxygen:

7.0 mg/l

Nominal and measured concentrations:

3.125 mg/L, 6.25 mg/L,12.5 mg/L, 25 mg/L and 50 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 50% mortality was observed

Sublethal observations / clinical signs:

Mortality and symptoms:

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3h		6 h		24 h		48h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
3.125	4	4	-	-	-	-	-	-	-	-	-	-	-	-
6.25	4	4	-	-	-	-	-	-	-	-	-	-	-	-
12.5	4	4	-	-	-	-	-	-	-	-	-	-	-	-
25	4	4	-	-	-	-	-	-	-	-	-	-	-	-
50	4	4	-	-	-	-	2	2	2	2	2	2	2	2

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

6 h

24 h

48h

72 h

96 h

Control

NS4

3.125

NS4

6.25

NS4

12.5

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

Nominal Concentration (mg/l)	Number of fish		pH readings after
			24 h		48h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.3	7.3	7.4	7.4	7.6	7.6	7.8	7.8
3.125	4	4	7.4	7.4	7.5	7.5	7.7	7.7	7.9	7.9
6.25	4	4	7.4	7.4	7.5	7.5	7.7	7.7	7.9	7.9
12.5	4	4	7.4	7.4	7.5	7.5	7.8	7.8	8.0	8.0
25	4	4	7.6	7.6	7.6	7.6	7.9	7.9	8.0	8.0
50	4	4	7.6	7.6	7.7	7.7	7.9	7.9	8.0	8.0

Nominal Concentration (mg/l)	Number of fish		Oxygen content (mg/L) after
			24 h		48h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	6.9	6.9	6.8	6.8	6.7	6.7	6.6	6.6
3.125	4	4	6.9	6.9	6.7	6.7	6.6	6.6	6.4	6.4
6.25	4	4	6.9	6.9	6.6	6.6	6.5	6.5	6.4	6.4
12.5	4	4	6.9	6.9	6.6	6.6	6.5	6.5	6.4	6.4
25	4	4	6.9	6.9	6.6	6.6	6.5	6.5	6.4	6.4
50	4	4	6.9	6.9	6.6	6.6	6.5	6.5	6.4	6.4

Test temperature:

Nominal Concentration (mg/l)	Number of fish		Temperature (°C) after
			24 h		48h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	23.4	23.4	23.3	23.3	23.1	23.1	23.5	23.5
3.125	4	4	23.4	23.4	23.3	23.3	23.1	23.1	23.5	23.5
6.25	4	4	23.4	23.4	23.3	23.3	23.1	23.1	23.5	23.5
12.5	4	4	23.4	23.4	23.3	23.3	23.1	23.1	23.5	23.5
25	4	4	23.4	23.4	23.3	23.3	23.1	23.1	23.5	23.5
50	4	4	23.4	23.4	23.3	23.3	23.1	23.1	23.5	23.5

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for 2-Methylanisole (578-58-5) on Zebra Fish Danio rerio was determine to be 50 mg/L.

Executive summary:

Study was conducted to assess the effect of test chemical 2-Methylanisole (578-58-5) on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was partially soluble in water. Therefore, the stock solution will be prepared by dissolving 1 g of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous 4 hour stirring.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item 2-Methylanisole (578-58-5) to various nominal test concentrations, LC50 was determine to be 50 mg/l . Based on the LC50, it can be consider that the chemical was hazardous and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

Description of key information

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for 2-Methylanisole (578-58-5) on Zebra Fish Danio rerio was determine to be 50 mg/L.

Key value for chemical safety assessment

Fresh water fish

Effect concentration:: 50 mg/L

Additional information

Based on the experimental Study which was conducted to assess the effect of test chemical 2-Methylanisole (578-58-5) on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was partially soluble in water. Therefore, the stock solution will be prepared by dissolving 1 g of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous 4 hour stirring.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item 2-Methylanisole (578-58-5) to various nominal test concentrations, LC50 was determine to be 50 mg/l . Based on the LC50, it can be consider that the chemical was hazardous and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

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