Undecan-1-ol

Sediment toxicity

Administrative data

Endpoint:

sediment toxicity: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Study conducted according to a modified OECD guideline, no mention of GLP, no analytical monitoring and lack of water quality measurements.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 850.1735 (Whole Sediment Acute Toxicity of Invertebrates, freshwater)

Deviations:

yes

Remarks:

the media used was soil and water

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Tetradecanol

EC Number:

204-000-3

EC Name:

Tetradecanol

Cas Number:

112-72-1

Molecular formula:

C14H30O

IUPAC Name:

tetradecan-1-ol

Test material form:

other: white, semi-solid

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

yes

Details on sediment and application:

PREPARATION OF SPIKED SEDIMENT

- Pooling or mixing of different substrates: soil and water

- Method of mixing: not reported

- Details of spiking: sand spike, using 10% of the total dry weight of soil. This involved adding an appropriate amount of compound to analar grade acetone to form a stock solution for the 1000 mg/kg dose. Aliquots of the stock solution were then diluted with acetone to prepare lower doses. Acetone solutions were added to silica sand in 500 ml amber glass jars. Mixtures were mixed thoroughly with a stainless steel spatula. The acetone was left to evaporate in a fume cupboard, leaving the test item coated onto the sand. Appropriate amount of moist soil was added to each of the glass jars. The dosed or undosed sand was incorporated into the bulk of the soil using a paletted knife. Jars were then sealed with screw top lids containing aluminium foil inserts and tumbled overnight (ca. 16 h) on a rotary soil tumbler 50 rpm.

- Equilibration time: not reported

- Controls: One aliquot of sand was dosed with acetone only to serve as solvent control. A jar with an aliquot of sand only served as untreated control - both with the appropriate amount of moist soil added thereafter.

- Chemical name of vehicle (organic solvent, emulsifier or dispersant):

- Concentration of vehicle in test medium (stock solution and final test solution): acetone solutions was added between 1-7 ml to 5-35g of silica sand. The sand was left to evaporate the acetone completely before initial exposure, so that the concentration of vehicle would be negligible.

- Evaporation of vehicle before use: yes


PREPARATION OF SPIKED WATER

- Details of spiking: not spiked

Test organisms

Test organisms (species):

other: Heterocypris incongruens

Details on test organisms:

TEST ORGANISM

- Common name: ostracods

- Justification for species other than prescribed by test guideline: testing new method

- Source: obtained as dormant cysts in test kits (Ostracodtoxkit F. Chronic "direct contact" Toxicity Test for Freshwater Sediments, MicroBio Tests Inc. Deinze, Belgium).

- Breeding conditions: as test kit instructed. Organism were hatched in standard hard water in a covered petri dish for 52 h at 25 degC before introduction to the prepared soil.

- Age of animals at beginning of exposure: a few hours old

- Feeding during test

- Food type: algae powder (Spirulina sp, provided with the toxkit)

- Amount: one tube

- Frequency: once, four hours before the test began



ACCLIMATION

- Acclimation period: none

Study design

Study type:

laboratory study

Test type:

static

Water media type:

freshwater

Limit test:

no

Exposure duration

Duration:

6 d

Exposure phase:

total exposure duration

Test conditions

Test temperature:

25 +/- 2 degC

pH:

not reported

Dissolved oxygen:

not reported

Nominal and measured concentrations:

Nominal concentrations: 0, 300, 1000 mg/kg dw soil

Details on test conditions:

TEST SYSTEM

- Test container (material, size): polystyerene, multi well plates, supplied with the Ostracodtoxkit F.

- Amount of soil or substrate: 300ul og either reference sediment (supplied with the kit) or treated soil

- No. of organisms per container (treatment): 10

- No. of replicates per treatment group: 3

- No. of replicates per control: 3

- No. of replicates per vehicle control: 3



SOURCE AND PROPERTIES OF SUBSTRATE

- Geographic location: Heath Farm, Leicester Lane, LandLook (Midlands), Leamington Spa, from Dr Graham Beard. OS map reference 32896926.

- Pesticide use history at the collection site: assumed to be pesticide free, as the field the soil was taken from land that had been set aside for 3-4 years.

- Collection procedures: the soil was sieved to 2 mm.

- Sampling depth (cm): 5-20cm to avoid surface root mat

- Soil texture

- % sand: 63.8%-64.8%

- Soil classification: sandy loam soil. The soil is described as soil series, Bomsgrove, standard soil number 33, variant.

- Organic carbon (%): 1.3%

- Maximum water holding capacity: ca. 40%

- Pretreatment of soil: the soil was treated with gamma radiation (27 KGy) in order to kill the indigenous nematodes and potential predators. However observation from extracted fresh soil indicated that interference from the indigenous population was likely to be insignificant. Consequently, the definitive test were carried out using unsterilised soil.

- Storage: stored in closed black plastic bags at 4 degC until required.


OTHER TEST CONDITIONS

- Photoperiod: plates were incubated in the dark

-Other: animals were fed with 3x10^7 algal cells


EFFECT PARAMETERS MEASURED: Growth was determined by measuring the length of ostracods at the beginning (on a sub-sample) and end of the exposure period (surviving individuals). Measured once the ostracods were fixed in Lugols solution and via a calibrated graticule under a binocular microscope. Mortality (immobilisation) was measured at the end of the test.


VEHICLE CONTROL PERFORMED: yes


TEST CONCENTRATIONS

- Spacing factor for test concentrations: 3

- Range finding study: no

Reference substance (positive control):

yes

Remarks:

dimethoate

Results and discussion

Effect concentrations

Duration:

6 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: reproduction and survival

Details on results:

- Mortality at end of exposure period: 0%

Results with reference substance (positive control):

- Results with reference substance valid? yes

- Relevant effect levels: 160 mg/kg dw soil

Reported statistics and error estimates:

Probit analysis (Finney 1971) was used to determine EC50 values and their corresponding 95% intervals in definitive tests.

Any other information on results incl. tables

Table 1. Summary of findings.

Concentration (mg/kg dw soil	Mortality (%)
Control	0
	10
	10
Acetone control	0
	0
	0
30	0
	0
	0
100	0
	0
	10
300	20
	10
	10
1000	40
	0
	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

on the data provided, however not enough data on water quality

Conclusions:

A 6 d EC50 value of >1000 mg/kg dw soil has been determined for the effects of the test substance on population numbers of the ostracod H. incongruens.