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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 203-778-1 | CAS number: 110-56-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1981-09-23
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute toxicities of organic chemicals to fathead minnows (pimephales promelas), Vol 1
- Author:
- Brooke LT, Call DJ, Geiger DL, Northcott CE (eds)
- Year:
- 1 984
- Bibliographic source:
- Center for Lake Superior Studies, University of Wisconsin-Superior. ISBN 0-9614968-0-0
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,4-dichlorobutane
- EC Number:
- 203-778-1
- EC Name:
- 1,4-dichlorobutane
- Cas Number:
- 110-56-5
- Molecular formula:
- C4H8Cl2
- IUPAC Name:
- 1,4-dichlorobutane
- Details on test material:
- - SMILES notation: CLCCCCCL
Constituent 1
- Specific details on test material used for the study:
- CAS No. 110-56-5
MW: 127.01
Purity: 97%
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken at 0 h and 96 h from all test chambers. From one test chamber duplicates were taken at 24 h, 48 h, 72 h and 96 h.
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Source: brood stock from U.S. EPA Environmental Research Laboratory-Duluth
- Age at study initiation (mean and range, SD): 30-31 days
- Weight at study initiation: 0.173 g
- Length at study initiation: 21.6 mm
- Acclimation conditions: Fish were held at 25 °C in flowing water with 16 h light-controlled photoperiod.
- Type and amount of food during acclimation: brine shrimp and dry flake. No feeding during exposure
- Health: Only fish with healthy appearance and no history of abnormally high mortality rate were used in the test.
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 44.5 mg/L CaCO3
- Test temperature:
- 24.6 °C
- pH:
- 7.68
- Dissolved oxygen:
- 6.8 mg/L
- Salinity:
- 41.5 mg/L CaCO3
- Nominal and measured concentrations:
- nominal 0, 25, 42, 69, 116, 193 mg/L. measured average concentrations from two tanks: 7.88 and 9.2 mg/L (25 mg/L nominal); 17.2 and 15.7 mg/L (42 mg/L nominal); 27.9 and 27.0 mg/L (69 mg/L); 48.8 and 52.7 mg/L (116 mg/L nominal); 74.3 and 88.8 mg/L (193 mg/L nominal).
- Details on test conditions:
- Tank volume 6.3 L, additions 4.7 v/d, 2 replicates per concentration. 25 fish per replicate, loading 0.687 g/L
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 51.6 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Conf. limit 47.8 - 55.6 mg/L
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 51.6 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- not specified
- Remarks on result:
- other: Conf. limit 47.8 - 55.6 mg/L
- Details on results:
- Affected fish became lethargic and stopped schooling. No reference to substance concentration were done.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The LC50 for Pimephales promelas after 96 h exposure has been determined to be 51.6 mg/L based on measured concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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