Methacrylamide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Japanese government peer-reviewed the documents, audited selected studies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Supplier: KISHIDA CHEMICALS CO.
Ltd (Lot No. D12358J)
Purity: >= 98 %

Analytical monitoring:

yes

Remarks:

measuerd by GC

Vehicle:

no

Test organisms (species):

Oryzias latipes

Details on test organisms:

Test Organism:
-supplier: Aichi Yatomi chiku fish farm (Aichi, Japan)
-size/weight: 21.2 mm (18.7 - 22.9 mm),
n= 10/0.1392 g (0.0913 - 0.1855 g),
n= 10 -feeding during acclimation: "TETRAMIN"
-acclimation: acclimated to dilution water for more than 12 days before testing
-feeding during test: none, feeding was stoped before 24 hr of the test

STOCK AND TEST SOLUTION AND THEIR PREPARATION:
-solvent: no solvent was used
-stock of solution: no data

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

41.0 mg/L as CaCO3

Test temperature:

23.1 - 23.8 °C

pH:

6.9 - 7.0

Dissolved oxygen:

6.4 - 11.3 mg/L

Nominal and measured concentrations:

Measured concentrations: 0 and 100 mg/L nominal concentration (actual concentration measured and greater than 80% of the nominal concentration):
Results:
Table 1 NOMINAL/MEASURED CONCENTRATION:

-----------------------------------------------------------
Nominal Measured conc. (mg/L)
conc (% of nominal)
(mg/L) 0 hr* 24 hr** mean
-----------------------------------------------------------
Control <0.5 <0.5
100 mg/L 90.5 (90.5) 94.4 (94.4) 92.4
-----------------------------------------------------------
* : freshly prepared test solution
**: test solution after 24 hr exposure period
EFFECT OF MORTALITY: none at control and 100 mg/L
SYMPTOMS: none of abnormalities at control and 100 mg/L

Details on test conditions:

DILUTION WATER:
-source: dechlorinated tap water
-aeration: no data
-hardness: 41.0 mg/L as CaCO3
-pH: 6.8

TEST SYSTEM
-concentration: 0, 100 mg/L
-detail test method: semi-static, open system
-renewal of test solution: 24 hr
-exposure vessel type: 3L glass vessel
-number of replicates/fish per replicate: 1/10
-test temperature: 23 - 25 °C
-intensity of irradiation: room light
-photoperiod: 16 hr - 8 hr light-dark cycle

TEST PARAMETER: mortality, abnormal behavior and abnormal respiration
SAMPLING: at 0 and 24 hr
MONITORING OF TEST SUBSTANCE CONCENTRATION: measured by GC

Reference substance (positive control):

yes

Remarks:

CuSO4-5H2O

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

EFFECT OF MORTALITY: none at control and 100 mg/L
SYMPTOMS: none of abnormalities at control and 100 mg/L

Results with reference substance (positive control):

- Results with reference substance valid?
- Mortality:
- LC50: 0.59 mg/L (96 h)

Reported statistics and error estimates:

Statistics: no data

Validity criteria fulfilled:

yes

Conclusions:

At a limit concentration of 100 mg/l (nomial), methacrylamide showed no mortality in a semistatic acute fish toxicity test OECD 203 with oryzias latipes.
LC50 (96h) > 100 mg/l (nominal)
Methacrylamide would be classified as not acute toxic to fish in accordance with CLP regulation

Executive summary:

In a 96-h acute toxicity study, Medaka (Oryzias latipes) were exposed to methacrylamide at nominal concentrations of > 100 mg/l without solvent under semistatic conditions. The 96-h LC50 was > 100 mg/l.

Based on the results on this study, methacrylamide would be classified as not acute toxic to fish in accordance with th classification CLP regulation.

This toxicity study is classified as acceptable and satifies the guideline for Fish, acute toxicity study (OECD 203)

Results Synopsis

Test organism size/age: mean length: 21.2mm; mean weight: 0.1392 g (n=10)

Test type: semistatic

LC50: > 100 mg/l measured/nominal

LC0: > 100 mg/l measured/nominal

LC100: > 100 mg/l measured/nominal

Endpoints effected: mortality, abnormal behavoir, abnormal respiration

Description of key information

In a 96-h acute toxicity study, Medaka (Oryzias latipes) were exposed to methacrylamide at nominal concentrations of > 100 mg/l without solvent under semistatic conditions. The 96-h LC50 was > 100 mg/l.

Based on the results on this study, methacrylamide would be classified as not acute toxic to fish in accordance with EU regulation No. 1272/2008 (CLP regulation).

This toxicity study is classified as acceptable and satifies the guideline for Fish, acute toxicity study (OECD 203)

LC50: > 100 mg/l measured/nominal

LC0: > 100 mg/l measured/nominal

LC100: > 100 mg/l measured/nominal

Endpoints effected: mortality, abnormal behavior, abnormal respiration

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L