Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 946-751-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- The test was conducted by means of Read Across approach. Further information was attached at section 13
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 7,16-dichloro-6,15-dihydroanthrazine-5,9,14,18-tetrone
- EC Number:
- 204-980-2
- EC Name:
- 7,16-dichloro-6,15-dihydroanthrazine-5,9,14,18-tetrone
- Cas Number:
- 130-20-1
- Molecular formula:
- C28H12Cl2N2O4
- IUPAC Name:
- 7,16-dichloro-6,15-dihydrodinaphtho[2,3-a:2',3'-h]phenazine-5,9,14,18-tetrone
- Test material form:
- solid: particulate/powder
- Details on test material:
- Vat Blue 6
Constituent 1
Method
- Target gene:
- Salmonella typhimurium histidine (his) reversion system
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix (Rat liver enzymes)
- Test concentrations with justification for top dose:
- 2, 20, 100, 300 µg per plate
The test substance is insoluble in water and organic solvents - Vehicle / solvent:
- anhydrous dimethylsulphoxide
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- Remarks:
- In addition, strains were periodically checked for deoxycholate sensitivity, penicillin sensitivity or resistance to the following mutagens TA1535 - methylnitrosourea TA1537 - 9 -aminoacridine TA98 - N-hydroxyaminofluorene TA100 - methylnitrosourea
- Details on test system and experimental conditions:
- A sample of the test item was tested in Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 at four dose levels: 2, 20, 100, 300 µg per plate. The compound, just prior to testing was dissolved in anhydrous dimethylsulphoxide. For assay, various amounts of the solution was added to give the appropriate concentration in 3.5 ml of molten soft-agar (0.9% Difco agar and 0.5% w/v of NaCl) containing 0.1 micromol biotin, 0.1 micromol histidine, 20 micromol glucose-6-phosphate, 3 micromol NADP+ , 25 micromol MgCl2, 300 micromol sodium phosphate buffer, pH 7.4 and 0.1 mL of nutrient broth culture of bacteria. Where liver activation was required, 0.1 mL of liver PMS was added just prior to overlaying onto plates containing 25 mL of minimal Davis agar. A positive control compound, acetylaminofluorene, was also tested using TA98. Negative controls contained all the above ingredients except the test chemical. Assays were carried out in duplicate.
After the agar had hardened, the plates were inverted and left to incubate at 37°C for 48 hrs. Counting was carried out using a New Brunswick Biotran II colony counter set at maximum sensitivity and the size setting left off. The counter had been previously calibrated using a test plate. Each experiment was repeated on two separate occasions. - Evaluation criteria:
- All results were meaned using a DEC-10 computer and the standard deviations calculated. Each test plate result was compared with the appropriate control using the SPSS programme for Students [-test for equal or unequal variances. Results in which the values statistically differ, i.e. p <=.0.05 are considered to be mutagenic
- Statistics:
- SPSS: Students [-test for equal or unequal variances
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium, other: TA1535, TA1537, TA100, TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- The test item was not mutagenic in the bacteria reverse mutation assay
- Executive summary:
The test item was tested with the Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 at concentrations from 2 to 300 µg per plate both in the presence and absence of metabolic activation. No mutagenic effect was observed
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.