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EC number: 210-233-1 | CAS number: 610-69-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.46 (In Vitro Skin Irritation: Reconstructed Human Epidermis Model Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: MatTek Protocol for: INVITRO EpiDermTM SKIN IRRITATION TEST For use with MatTek Corporation’s Reconstructed Human Epidermal Model EpiDerm, Model EPI- 200-SIT, Rev. 26/3/2012,1-37
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Acetic acid, 2-nitrophenyl ester
- IUPAC Name:
- Acetic acid, 2-nitrophenyl ester
- Reference substance name:
- 2-nitrophenyl acetate
- EC Number:
- 210-233-1
- EC Name:
- 2-nitrophenyl acetate
- Cas Number:
- 610-69-5
- Molecular formula:
- C8H7NO4
- IUPAC Name:
- 2-nitrophenyl acetate
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material (as cited in study report): 2-Nitrophenyl Acetate
- Physical state: solid light yellow
- Analytical purity: 95 % w/w
- Impurities (identity and concentrations):
Phenol <5.0 % (w/w)
2,4-dinitrophenol <5.0 % (w/w)
2,6-dinitrophenol <5.0 % (w/w)
Acetic acid <1.0 % (w/w)
Water <5.0 % (w/w)
2-nitrophenol <5.0 % (w/w)
- Lot/batch No.: 201408
- Expiration date of the lot/batch: unlisted
- Storage condition of test material: The test substance will be stored in dry room in dark in
closed container at the room temperature
Constituent 1
Constituent 2
Test animals
- Species:
- other: reconstructed human epidermal model EpiDerm™ (EPI-200 ver. 2.0, MatTek, Ashland, USA); Lot No. 21646, kit A
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- not specified
- Vehicle:
- not specified
- Duration of treatment / exposure:
- 60 minutes
Results and discussion
Any other information on results incl. tables
OD570 measuring was performed after 2-hour extraction with shaking. Results are given in the Table 1. Extracts as well as tissues after extraction are given in Figure 2.
Table 1: OD570 values obtained at the MTT test, their means, standard deviations and relative viabilities (%)
Treatment | OD570 | Mean | SD | Relative viability | |||
1 | 2 | 3 | (% NC) | ||||
NC | PBS | 1.874 | 1.976 | 2.015 | 1.955 | 0.059 | 100.0 |
viability (% NC) | 95.86 | 101.07 | 103.07 | 100.00 | 3.041 | ||
C2 | 66/15 | 2.309 | 2.010 | 2.020 | 2.113 | 0.139 | 108.1 |
viability (% NC) | 118.11 | 102.81 | 103.32 | 108.08 | 7.092 | ||
PC | 5% SDS | 0.049 | 0.045 | 0.045 | 0.046 | 0.002 | 2.4 |
viability (% NC) | 2.51 | 2.30 | 2.30 | 2.37 | 0.096 |
NC = negative control
PC = positive control
C2 = test substance
mean = arithmetic mean
SD = standard deviation calculated from individual % tissue viabilities
viability (%) = viability of single tissues compared with negative control, mean NC viability is defined as 100
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The effect of the test substance was negative in EpiDermTM model.
- Executive summary:
Test substance, 2-Nitrophenyl Acetate, was assayed for the in vitro skin irritation in human epidermal model EpiDermTM. The test was performed according to the Method B.46. In vitro skin irritation: Reconstructed human epidermis model test and Protocol for: In Vitro EpiDermTM Skin Irritation Test For use with MatTek Corporation’s Reconstructed Human Epidermal Model EPI-200-SIT (see par. 1.4, (1), (3)).
After pre-incubation of tissues, 25 mg of the test was placed directly atop to the previously moistened tissue so it covered all tissue surfaces. Length of exposition was 60 minutes. Three tissues were used for every concentration and controls.
After removal of the test substance from tissues, tissues were post incubated for 42 hours due to leave of damage reparation. Three hours incubation with MTT and two hours extraction period with shaking followed then. Optical density (OD570) of isopropyl alcohol extracts was measured on a spectrophotometer. Relative cell viability was calculated for each tissue as % of the mean viability of the negative control tissues.
Under the above-described experimental design, average viability of tissues treated by the test substance was 108.1 %, i.e.viability was > 50 %.
The effect of the test substance was negative in EpiDermTM model (the tissue was not damaged).
According to the classification criteria given in chapter 3.9., the test substance is considered to have no category in regard to skin irritation.
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