Registration Dossier

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Results of the harmful effects of water pollutants to Daphnia Magna in the 21 day reproduction test
Author:
R. Kuhn, M.Pattard, Klaus-Dieter Pernak, Angela Winter
Year:
1989
Bibliographic source:
Wat. Res. Vol. 23, No 4, pp 501-510

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: "Provisional Procedure: extended toxicology test with Daphnia magna (determination of NOEC for reproduction rate, mortality and the time of the first aapearance of offspring; 21d)", Federal Environmental Agency, 1 January 1984.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: crystalline

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Diluition water
An artificial medium (synthetic fresh water) of the following composition was used in the test and control preparations:
11.76 g CaCl2•2H2O (A.R.)/1 litre deionized water
4.93 g MgSO4•7H2O (A.R.)/1 litre deionized water
2.59 g NaHCO3 (A.R.)/1 litre deionized water
0.23 g KCl (A.R.)/1 litre deionized water
Twenty-five millilitres of each solution was pipetted into a graduated flask and completed to 1 litre with deionized water.
This water was aerated up to the water saturation level and the pH value was measured (8.0±0.2)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Dapnia magna strain (IRCHA strain).
20-30 specimens were placed in forty 2-l beakers which had been filled with at least 1.6 l Berlin tap water. They provided 24h-old animals when the offspring were removed daily from the cultures.
For all Daphnia strain cultures, temperature-controlled, dechlorinated and oxygen satured tap water (German hardness 16°, pH value 7.6-7.7) was used which had been left to stand for 24h. Feeding with dry algae of the Scenedesmus genus took place daily. The temperature of the culture area was regulated at 20°C.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

pH:
8.0±0.2

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
other: EC0
Effect conc.:
1.8 mg/L
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
3.4 mg/L
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.1 mg/L
Basis for effect:
other: Based on the reproduction rate and appearance of first offspring.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
21 days NOEC on Daphnia magna was determined to be 0.1 mg/L.