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EC number: 200-625-0 | CAS number: 66-27-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- Data is from publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- Local Lymph Node Assay: Validation Assessment for Regulatory Purposes.
- Author:
- G. Frank Gerberick, Cintfy A. Ryan, Ian Kimber, Rebecca]. Dearman, Linda]. Lea, and DavidA. Basketter
- Year:
- 2 000
- Bibliographic source:
- American Journal of Contact Dermatitis 2000 Mar; 11(1):3-18.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Principles of method if other than guideline:
- In Local Lymph Node Assay, the Methyl methanesulphonate is administered on three consecutive days, followed by a 2-day rest period before analysis. On day 6 (5 days after initiation of treatment), the mice are injected intravenously, by the tail vein, with 250 μL of sterile phosphate-buffered saline (PBS) containing 20 μCi of [3H]methyl thymidine and the lymph nodes are excised five hours later. A lymph node cell suspension is then prepared and tritium thymidine or iododeoxyuridine 125 incorporation is determined by scintillation counting.
- GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Methyl methanesulphonate
- EC Number:
- 200-625-0
- EC Name:
- Methyl methanesulphonate
- Cas Number:
- 66-27-3
- Molecular formula:
- C2H6O3S
- IUPAC Name:
- methyl methanesulfonate
- Details on test material:
- - Name of test material (as cited in study report): Methyl methanesulfonate (MMS)
- Molecular formula (if other than submission substance): C2H6O3S
- Molecular weight (if other than submission substance): 110.1324 g/mole
- Substance type: Organic
- Physical state: Liquid
Purity: >95 %
- Impurities (identity and concentrations): , 5%
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Methyl methanesulphonate
- Molecular formula (if other than submission substance): C2H6O3S
- Molecular weight (if other than submission substance): 110.132 g/mol
- Substance type: organic
- Physical state: solid
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA/Ca
- Sex:
- female
- Details on test animals and environmental conditions:
- Details on test animal
TEST ANIMALS
- Source:
- Age at study initiation: 6 to 16 wk-old
- Weight at study initiation: no data
- Housing: no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr):no data
- Photoperiod (hrs dark / hrs light): no data
IN-LIFE DATES: From: To: no data
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 25 μL
- No. of animals per dose:
- 4-5 animals
- Details on study design:
- Details on study design
PRE-SCREEN TESTS:
- Compound solubility: no data
- Irritation: no data
- Systemic toxicity: no data
- Ear thickness measurements: no data
- Erythema scores: >3
MAIN STUDY
ANIMAL ASSIGNMENT AND TREATMENT
- Name of test method: Local Lymph node asssay
- Criteria used to consider a positive response: Si > 3
•TREATMENT PREPARATION AND ADMINISTRATION:
Groups of mice (n 4 or 5) are treated by topical application, on the dorsum of both ears, with 25 μL of 1 of several concentrations (100%,50%,25%, 10%, 5%, 2.5%, 1%,0.5%, 0.25%, and 0.1 %) of test material, or with an equal volume of the relevant vehicle alone.
They are treated daily for 3 consecutive days, followed by a 2 day rest period before analysis. On the sixth day (5 days after initiation of treatment), the mice are injected intravenously, by the tail vein, with 250 μL of sterile phosphate-buffered saline (PBS) containing 20 μCi of [3H] methyl thymidine. Five hours later, the mice are killed, and the draining auricular lymph nodes are excised and pooled for each experimental group or for each individual animal. Single cell suspensions of lymph node cells are prepared. Lymph node cells are washed twice with an excess of PBS and precipitated with 5% trichloroacetic acid (TCA) at 4°C. The samples, pelleted by centrifugation, are resuspended 12 to 18 hours later in 1mL of 5% TCA and transferred to 10 mL of scintillation cocktail. Incorporation of tritiumlabeled thymidine [3H-TdR] is measured by beta-scintillation counting and expressed as disintegrations per minute (dpm). - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- The data are expressed as mean dpm for each experimental group, and the Stimulation Index (SI) is derived for each experimental group by dividing the mean dpm of that group by the mean dpm of the vehicle-control group. Experimental groups are compared with vehicle-treated controls.
SI of 3 (EC3) can be calculated EC3 determination is that data from the entire dose response curve are used to produce a single value of intrinsic potency.14 The EC3 value can be used to rank the relative skin-sensitizing potential of chemicals.
Results and discussion
In vivo (LLNA)
Results
- Key result
- Parameter:
- SI
- Value:
- > 3
- Test group / Remarks:
- Yes
- Remarks on result:
- other: Positive indication of skin sensitization .
- Cellular proliferation data / Observations:
- CELLULAR PROLIFERATION DATA/observation
CELLULAR PROLIFERATION DATA
The stimulation indices (SIs) for each experimental group are determined as the increase in 3H-TdR incorporation relative to concurrent vehicle-treated controls. A Methyl methanesulphonate that, at 1 or more concentrations, causes an SI of 3 or greater is considered to have skin-sensitizing activity. Thus, whether the draining auricular lymph nodes are excised and pooled for each experimental group or for each individual animal, the three-fold or greater increase in proliferative activity compared with concurrent vehicle-treated control animals is the sole criterion for a classification of skin-sensitizing activity.
DETAILS ON STIMULATION INDEX CALCULATION
Stimulation indices (SIs) for each experimental group are determined as the increase in 3H-TdR incorporation relative to concurrent vehicle-treated controls. A Methyl methanesulphonate that, at 1or more concentrations, causes an SI of 3 or greater is considered to have skin-sensitizing activity.
EC3 CALCULATION:
The EC3 values (3-fold increase in stimulation index)
CLINICAL OBSERVATIONS: no data
BODY WEIGHTS
No data
Applicant's summary and conclusion
- Interpretation of results:
- other: sensitizing
- Conclusions:
- Methyl methanesulphonate(66-27-3) was observed for its skin sensitization potential in mouse by LLNA mode .It is concluded from the Mouse local lymphnode assay (LLNA) in vivo test of skin sensitization potential for Methyl methanesulphonate is skin sensitizing to the female CBA/Ca mouse.
- Executive summary:
The Mouse local lymphnode assay (LLNA) in vivo test was performed on 6-16 weeks old, 4-5 female CBA/Ca mice to study the skin senisitization potential of Methyl methanesulphonate(66-27-3).Groups of mice (n 4 or 5) are treated by topical application, on the dorsum of both ears, with 25μLof 1of several concentrations of test material, or with an equal volume of the relevant vehicle alone. They are treated daily for 3 consecutive days, followed by a 2 day rest period before analysis. On the sixth day (5 days after initiation of treatment), the mice are injected intravenously, by the tail vein, with 250μLof sterile phosphate-buffered saline (PBS) containing 20μCi of [3H] methyl thymidine. Five hours later, the mice are killed, and the draining auricular lymph nodes are excised and pooled for each experimental group or for each individual animal. Single cell suspensions of lymph node cells are prepared. Lymph node cells are washed twice with an excess of PBS and precipitated with 5% trichloroacetic acid (TCA) at 4°C. The samples, pelleted by centrifugation, are resuspended 12 to 18 hours later in 1mL of 5% TCA and transferred to 10 mL of scintillation cocktail. Incorporation of tritiumlabeled thymidine [3H-TdR] is measured by {beta-scintillation counting and expressed as disintegrations per minute (dpm). The drainined auricular lymph nodes are excised and pooled for each experimental group or for each individual animal, the three-fold or greater increase in proliferative activity compared with concurrent vehicle-treated control animals is the sole criterion for a classification of skin-sensitizing activity.
Thus from the result obtained from the The Mouse local lymphnode assay (LLNA) in vivo test on mice for Methyl methanesulphonate,at 1or more concentrations, causes an SI of 3 or greater is considered to have skin-sensitizing activity. ThereforeMethyl methanesulphonate(66-27-3) was considered to be sensitizing to the female CBA/Ca mouse.
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