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EC number: 857-673-6 | CAS number: 1879067-61-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study Initiation Date: September 02, 2019 Experimental Starting Date: September 09, 2019 Experimental Completion Date: November 26, 2019 Study Completion Date: January 28, 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study according to OECD guideline 202 and under GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Swiss GLP Monitoring Authorities, Date of decission 2018-01-10
- Specific details on test material used for the study:
- Appearance: Beige solid
Expiration Date: End of February 2021
Storage Conditions: < 30°C - Analytical monitoring:
- yes
- Details on sampling:
- - Sampled concentrations: 4.6, 10, 22, 46, and 100 mg/L
- Sampling method: duplicate samples were taken from each test concentration and the control at
the start and at the end of the test (after 48 hours). For the 48-hour stability samples, the contents
of the respective replicates were combined prior to sampling.
- Sample storage conditions before analysis: all samples were frozen (at -20 ± 5 °C)
- Analysed samples: The concentrations of the test item CA5727A were measured in one of the duplicates for all taken samples. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test medium of the highest nominal concentration of 100 mg/L was prepared by mixing
100.16 mg of the test item into 1000 mL of test water using ultrasonic treatment for
15 minutes and intense stirring for 15 minutes at room temperature.
- Eluate:The highest test concentration of 100 mg/L was further diluted with test water to prepare the test
media with the lower test concentrations. The test medium was prepared just before the introduction of
the daphnids (i.e., start of the test).
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No remarkable observations were made concerning the appearance of the test media.
All test media were clear solutions throughout the entire test duration. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Daphnia magna Straus
- Source: Daphnia Collection of the University of Basel/Switzerland in 2015
- Age of parental stock: 3-4 Weeks. At the start of the test, the organisms used
in the test were 6 to 24 hours old and were not first brood progeny.
- Feeding during test: no
ACCLIMATION
- Acclimation period: no acclimatisation was necessary, since the bred of the daphnia
was under identical conditions to those of the test.
- Type and amount of food during the breeding: Algal suspension of the green algae
Desmodesmus subspicatus supplied by the Collection of Algal Cultures (SAG, Institute for
Plant Physiology, University of Göttingen, 37073 Göttingen / Germany) and cultivated at
IES Ltd Laboratories under standardized conditions or a mixture of this algal suspension and a
commercial fish diet (Tetra Min® Hauptfutter, supplied by TETRA-GmbH, 49324 Melle / Germany).
- Feeding frequency: three times a week
- Health during acclimation (any mortality observed): The daphnia were healthy, no mortality was observed. - Test type:
- static
- Water media type:
- other: ISO Test water (Medium according to the guideline)
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.5 mmol/L = 250 mg/L as CaCO3
- Test temperature:
- 20-21 °C
- pH:
- 7.8
- Dissolved oxygen:
- at the start of the test at all concentrations and the control: 8.4-8.7 mg/L oxygen
at the end of the test at all concentrations and the control: 8.3-8.7 mg/L oxygen - Salinity:
- n.d.
- Conductivity:
- n.d.
- Nominal and measured concentrations:
- nominal concentrations (measured concentrations 0/48h): 4.6 (4.6/4.9), 10 (10/11), 22 (22/23), 46 (46/46) and
100 (103/99) mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Beakers
- Type (delete if not applicable): Open (The test vessels were Loosely coverd with glass sheets
to reduce the loss of water by evaporation and to avoid the entry of dust into the solutions.)
- Material, size, headspace, fill volume: glas, 100 mL, open, 50 mL
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): no, static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4
- No. of vessels per vehicle control (replicates):no
- Biomass loading rate: 1 Daphnia / 10 mL test media
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO Medium
Ingredients / Properties [mmol/L] / Concentration [mg/L]
CaCl2 × 2H2O / 2.0 / 294
MgSO4 × 7H2O / 0.5 / 123
NaHCO3 / 0.75 / 65
KCl / 0.075 / 5.8
- Alkalinity: 0.8 mmol/L
- Water Hardness: 2.5 mmol/L = 250 mg/L as CaCO3
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod:16-hour light to 8-hour dark cycle with a 30-minute transition period
- Light intensity:16 and 17 µE m-2 s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility of the daphnids and other non-lethal effects (e.g. abnormal behavior or appearance) after 24 and 48 hours of exposure.
VEHICLE CONTROL PERFORMED: no, not needed
RANGE-FINDING STUDY:
- Test concentrations: 1.0 / 10 / 100 mg/L
- Results used to determine the conditions for the definitive study:
48-hour immobilization: 1.0 mg/L / 0%; 10 mg/L / 20%; 100 mg/L / 100% - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate / Separate reference studies under GLP twice a year.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no abnormalities in the control and up and including the highest test concentration
100 mg/L.
- Mortality of control: no
- Other adverse effects control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: 0.19-3.0 mg/L
- Dose-response test: Yes
- ECx: 24-hour EC50: 1.8 mg/L
48-hour EC50: 1.4 mg/L - Reported statistics and error estimates:
- No statistics needed.
The 24-hour and 48-hour NOEC were determined directly from the raw data. The
24-hour and 48-hour EC50 of the test item could not be calculated, because none of the responses
exceeded 50%. The EC50 was, therefore, also determined directly from the raw data. - Validity criteria fulfilled:
- yes
- Conclusions:
- The substance had not adverse effects on aquatic freshwater invertebrates at a test concentration of 100 mg/L over a test period of 48 hours.
- Executive summary:
The acute toxicity of the test item to freshwater invertebrates (Daphnia magna) was determined in a 48‑hour static test according to the OECD Guideline for Testing of Chemicals, No. 202 (2004) and the Commission Regulation (EC) No. 440/2008, Part C.2.
The nominal test item concentrations tested were 4.6, 10, 22, 46 and 100 mg/L. A control (test water without test item) was tested in parallel. The measured concentrations of the test item in the test mediawith the concentrations from 4.6 to 100 mg/L were between 98 and 103% of the nominal values at the start of the test and between 99 and 107 % of nominal at the end of the test. The reported biological results were based on the nominal concentrations of the test item, since the correct dosage and the stability of the test item were confirmed.
The biological test results (based on nominal concentrations) were as follows:
48-hour EC50 >100 mg/L
48-hour NOEC = 100 mg/L
Reference
Analytical results
Sampling Day/ |
Nominal Concentra-tion of |
Measured Concentration |
Sample Preparation Factor |
Determined Concentration of Test Item |
% of Nominal Concentration |
|
[day/hours] |
[mg/L] |
[µg/L] |
|
[mg/L] |
[%] |
|
0/0 |
Control |
<LOD |
42 |
n.a. |
n.a. |
|
|
4.6 |
3.62 |
1260 |
4.56 |
99 |
|
|
10 |
3.70 |
2772 |
10.3 |
103 |
|
|
22 |
3.28 |
6552 |
21.5 |
98 |
|
|
46 |
3.60 |
12852 |
46.3 |
101 |
|
|
100 |
4.04 |
25452 |
103 |
103 |
|
2/48 |
Control |
<LOD |
42 |
n.a. |
n.a. |
|
|
4.6 |
3.85 |
1260 |
4.85 |
105 |
|
|
10 |
3.88 |
2772 |
10.7 |
107 |
|
|
22 |
3.43 |
6552 |
22.5 |
102 |
|
|
46 |
3.60 |
12852 |
46.3 |
101 |
|
|
100 |
3.89 |
25452 |
99.0 |
99 |
LOD = 0.0497 µg test item/L
n.a. = not applicable
The tabulated values of the samples represent rounded results obtained by calculation using the exact Raw Data.
Biological Results: Effect of CA5727A on the Mobility of Daphnia magna
Nominal Test Item Concentration |
No. of Daphnids Tested |
Immobilized Daphnids after 24 Hours |
Immobilized Daphnids after 24 Hours |
Immobilized Daphnids after 48 Hours |
Immobilized Daphnids after 48 Hours |
[mg/L] |
|
No. |
[%] |
No. |
[%] |
Control |
20 |
0 |
0 |
0 |
0 |
4.6 |
20 |
0 |
0 |
0 |
0 |
10 |
20 |
0 |
0 |
0 |
0 |
22 |
20 |
0 |
0 |
0 |
0 |
46 |
20 |
0 |
0 |
0 |
0 |
100 |
20 |
0(3F) |
0 |
0 |
0 |
F: number of mobile test animals with slower swimming activity compared to the control animals
Description of key information
48-hour EC50 >100 mg/L (Daphnia magna, OECD TG 202, GLP)
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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