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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2020-03-31 to 2020-06-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- Based on the predicted moisture content of artificial soil approximately 30 % and the purity of the test substance was 98.5%, respectively 586 mg of test substance was weighed and mixed well with quartz sand to a total weight of about 10 g by grinding. Each mixture was then mixed with about 740 g wet artificial soil to obtain test substance concentrations of 1000 mg/kg (dry weight of atrtificial soil). Four replicates were prepared. Blank control was prepared by mixing about 10 g of quartz sand and about 740 g wet artificial soil, with four replicates.
The above test media (750 g wet weight) was transferred into 1L glass beakers. Ten healthy earthwonns which had been washed and dried with filter paper before exposure were placed randomly on the surface of artificial soil in each container. These earthworms were placed on the surface and allowed to burrow into the test medium by themselves. The containers were covered with perforated plastic film to prevent the artificial soil from drying.
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- The earthworms which were used in the test were purchased on February 20, 2020, and all earthwonns have been raised in the laboratory for 40 days before use.
Before the test, earthworms were acclimated to the study conditions for about 24 hours in the aitificial soil.
Batch No. EEf20200220-I
Weight 521 mg - 596 mg per earthwom
Age Older than 2 months
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 20°C ± 2°C
- pH:
- 5.84 (at the beginning of the test)
6.32 - 6.37 (at the end of the test) - Moisture:
- 29.7 % (dry weight, at the beginning of the test)
28.2% - 28.8% (dry weight, at the end of the test) - Details on test conditions:
- The test systems were put into an incubator to cultivate. During the test, the light intensity was 400 1x - 800 1x with continuous light, the environment temperature was 20°C±2°C, and the humidity was 65% - 100%. The test duration was 14 days.
- Nominal and measured concentrations:
- Nominal: 1000 mg/kg (dry weight of atiificial soil).
- Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide (C2H4ClNO)
Results and discussion
Effect concentrations
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- After 7 days and 14 days exposure, the mortalities of earthworms in the blank control were both 0%, and in the 1000 mg/kg (dry weight of artificial soil) test group were both 0%.
- Results with reference substance (positive control):
- The sensitivity test was carried out with the reference substance chloroacetamide (C2H4ClNO) based on the artificial soil test of the same batch Eisenia foetida. The 14 d-LC50 of chloroacetamide was 27.6 mg/kg (dry
weight of artificial soil), 95% confidence limit was 25.7 mg/kg - 29.7 mg/kg.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The mortality of earthworms in blank control was 0% at the end of the test, which fulfilled the criteria for the validity of not exceeding 10%.
- Conclusions:
- Under the test conditions, the result for the 14 d median lethal concentration (LC50) was greater than 1000 mg/kg (dry weight of artificial soil).
- Executive summary:
The artificial soil test involves keeping earthworms in samples of a precisely defined artificial soil, in which 1000 mg/kg (dry weight of artificial soil) of the test substance had been applied.
Mortality was assessed 7 days and I 4 days after application. Then the acute toxicity of the test substance was detennined by the results of the mortality.
After 7 days and 14 days exposure, the mortalities of earthworms in the blank control were both 0%, and in the 1000 mg/kg (dry weight of artificial soil) test group were both 0%.
Under the test conditions, the result for the 14 d median lethal concentration (LC50) was greater than 1000 mg/kg ( dry weight of artificial soil ).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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