Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: 1309389-73-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-01-20 to 2016-02-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- signed 2014-04-30
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: conventional treatment plant for domestic waste, Shenyang Northern Treatment Plant, China
- Preparation of inoculum for exposure: coarse particles were removed by filtration through a fine sieve and the sample was settled. After the supernatant was discarded, the sludge was washed twice with mineral medium and the concentrated sludge was suspended in mineral medium. 3 replicates of 10 ml suspended sludge were weighed and dried at 105°C for 125 min and reweighed to calculate the initial sludge concentration.
- Concentration of sludge: measured concentration of suspended sludge was 5.05 g/L as suspended solids (SS), which was adjusted to 4.04 g/L as suspended solids (SS). On the day of the test start, five replicates of 10 ml sludge were weighed and dried at 105°C for about 95 min and reweighed. The mean concentration of suspended sludge measured was 3806 mg/L SS.
- 7.880 mL of activated sludge was added to 1 L flask and made up to volume to give a suspended solid concentration of 30 mg/L.
- Storage length/ storage conditions: kept aerobic at 20.9-22.7°C until used for the test - Duration of test (contact time):
- 28 d
- Initial conc.:
- 41.6 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
a) KH2PO4 (8.52 g/L), K2HPO4 (21.75 g/L), Na2HPO4 × 2H2O (33.40 g/L), NH4Cl (0.50 g/L)
b) CaCl2 (27.50 g/L)
c) MgSO4 × 7H2O (22.51 g/L)
d) FeCl3 × 6H2O (0.25 g/L)
- Test temperature: 22 °C +/- 1 °C
- pH: start of test: 7.49; end of test: 7.74 - 8.08
- Continuous darkness: diffuse light
TEST SYSTEM
- Culturing apparatus: 1-L flasks
- Number of culture flasks/concentration: 2 flasks each for test item and inoculum control; one flask each for procedure control and toxicity control
- Measuring equipment: oxygen uptake was measured in regular intervals by an automated respirometer (CES company)
- Incubation: 28 days under diffuse light
SAMPLING
- Sampling frequency: Oxygen uptake was recorded by an automated respirometer in all test flasks at 24 hour intervals for 28 days. Evolved CO2 was absorbed in a solution of sodium hydroxide.
- The pH of various test solutions was measured at the end of the incubation period on Day 28.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (2 replicates, containing only inoculum)
- Toxicity control: yes (1 replicate; 41.8 mg/L test substance; equivalent to 93.6 mg/L ThOD and 55.7 mg/L reference substance; equivalent to 93.0 mg/L ThOD resulting in total ThOD of 186.6 mg/L)
- Procedure control: yes (1 replicate; 56.9 mg/L reference substance; equivalent to 95.0 mg/L ThOD) - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Purity: 99.6 %; 56.9 mg/L medium
- Preliminary study:
- not applicable
- Test performance:
- No unusual observation during tests.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1.6
- Sampling time:
- 28 d
- Details on results:
- The main biodegradation of test item after 28 days amounts to 1.6 % (2 replicates).
Biodegradation of the reference substance (Sodium benzoate): 63.5, 87.7 and 92.9 % after Day 4, 14 and 28, respectively.
Toxicity Control: 44.2 % degradation within 14 days exceeding 25 % based on total ThOD indicating that the test item is not inhibitory under the test conditions.
In conclusion, according to the guideline, the test item is not readily biodegradable under the test conditions.
Validity: The test is valid, as
- the difference between extremes of replicates of the removal of the test item at the plateau and at test end was less than 20 %,
- the percentage degradation of the reference substance (with 87.7 %) exceeded pass level of 60 % by day 14,
- 44.2 % and thus more than 25 % degradation based on total ThOD occured in toxicity control within 14 days,
- the oxygen uptake of the inoculum blank was 22.0 mg O2/L and thus < 60 mg/L in 28 days, and
- the pH remained within the range of 6.0 - 8.5. - Results with reference substance:
- The reference substance sodium benzoate degraded up to 87.7 % within the first 14 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The substance is not biodegradable after 28 days of exposure under conditions of the Manometric Respirometry Test (OECD 301 F). Further, 41.8 mg/L of the substance did not inhibit the biodegradataion of the reference substance sodium benzoate.
Reference
Table 1 Biodegradation Results of Test Item (28 days)
|
02 -uptake |
BOD (mgO2/mg test item) |
|
Biodegradation |
|||||||
Day |
IC-1 |
IC-2 |
TS-1 |
TS-2 |
IC Average |
TS-1 |
TS-2 |
TS Average |
TS-1 |
TS-2 |
TS Average |
1 |
2.29 |
2.79 |
2.83 |
3.25 |
2.5 |
0.008 |
0.018 |
0.013 |
0.4% |
0.8% |
0.6% |
2 |
4.44 |
5.04 |
4.85 |
5.79 |
4.7 |
0.004 |
0.026 |
0.015 |
0.2% |
1.2% |
0.7% |
3 |
6.29 |
6.85 |
7.08 |
8.12 |
6.6 |
0.012 |
0.037 |
0.025 |
0.5% |
1.7% |
1.1% |
4 |
8.06 |
8.60 |
8.70 |
10.18 |
8.3 |
0.010 |
0.045 |
0,028 |
0.4% |
2.0% |
1.2% |
5 |
9.91 |
10.35 |
10.35 |
12.08 |
10.1 |
0.006 |
0.048 |
0.027 |
0.3% |
2.1% |
1.2% |
6 |
11.12 |
11.54 |
11.47 |
13.41 |
11.3 |
0.004 |
0.051 |
0.028 |
0.2% |
2.3% |
1.3% |
7 |
11.45 |
11.93 |
11.95 |
13.97 |
11.7 |
0.006 |
0.055 |
0.031 |
0.3% |
2.5% |
1.4% |
8 |
12.00 |
12.83 |
12.74 |
14.97 |
12.4 |
0.008 |
0.062 |
0.035 |
0.4% |
2.8% |
1.6% |
9 |
12.43 |
13.24 |
13.16 |
15.76 |
12.8 |
0.009 |
0.071 |
0.040 |
0.4% |
3.2% |
1.8% |
10 |
13.04 |
13.85 |
13.79 |
16.64 |
13.4 |
0.009 |
0.078 |
0.044 |
0.4% |
3.5% |
2.0% |
11 |
13.62 |
14.37 |
14.41 |
17.35 |
14.0 |
0.010 |
0.080 |
0.045 |
0.4% |
3.6% |
2.0% |
12 |
14.20 |
14.89 |
15.10 |
18.03 |
14.5 |
0.014 |
0.085 |
0.050 |
0.6% |
3.8% |
2.2% |
13 |
15.20 |
15.66 |
15.68 |
19.01 |
15.4 |
0.007 |
0.087 |
0.047 |
0.3% |
3.9% |
2.1% |
14 |
15.76 |
16.29 |
16.39 |
19.62 |
16.0 |
0.009 |
0.087 |
0.048 |
0.4% |
3.9% |
2.2% |
15 |
16.06 |
16.45 |
16.39 |
19.89 |
16.3 |
0.002 |
0.086 |
0.044 |
0.1% |
3.8% |
2.0% |
16 |
16.33 |
16.74 |
16.60 |
20.30 |
16.5 |
0.002 |
0.091 |
0.047 |
0.1% |
4.1% |
2.1% |
17 |
16.95 |
17.45 |
17.14 |
20.93 |
17.2 |
0.000 |
0.090 |
0.045 |
0.0% |
4.0% |
2.0% |
18 |
17.37 |
17.93 |
17.58 |
21.43 |
17.7 |
0.000 |
0.090 |
0.045 |
0.0% |
4.0% |
2.0% |
19 |
17.64 |
18.37 |
17.87 |
21.80 |
18.0 |
0.000 |
0.091 |
0.046 |
0.0% |
4.1% |
2.1% |
20 |
18.12 |
18.97 |
18.26 |
22.26 |
18.5 |
0.000 |
0.090 |
0.045 |
0.0% |
4.0% |
2.0% |
21 |
18.72 |
19.60 |
18.66 |
22.78 |
19.2 |
0.000 |
0.086 |
0.043 |
0.0% |
3.8% |
1.9% |
22 |
19.16 |
20.10 |
19.10 |
23.12 |
19.6 |
0.000 |
0.085 |
0.043 |
0.0% |
3.8% |
1.9% |
23 |
19.53 |
20.41 |
19.26 |
23.30 |
20.0 |
0.000 |
0.079 |
0.040 |
0.0% |
3.5% |
1.8% |
24 |
19.62 |
20.66 |
19.89 |
24.03 |
20.1 |
0.000 |
0.094 |
0.047 |
0.0% |
4.2% |
2.1% |
25 |
20.62 |
21.20 |
20.14 |
24.22 |
20.9 |
0.000 |
0.080 |
0.040 |
0.0% |
3.6% |
1.8% |
26 |
20.80 |
21.32 |
20.33 |
24.28 |
21.1 |
0.000 |
0.076 |
0.038 |
0.0% |
3.4% |
1.7% |
27 |
21.53 |
21.78 |
20.64 |
24.62 |
21.7 |
0.000 |
0.070 |
0.035 |
0.0% |
3.1% |
1.6% |
28 |
21.87 |
22.10 |
21.03 |
24.93 |
22.0 |
0.000 |
0.070 |
0.035 |
0.0% |
3.1% |
1.6% |
Note: IC = Incentum Control; TS = Test Suspension.
Description of key information
Biodegradation in water - manometric respirometry test (OECD 301F; GLP compliant): not readily biodegradable (1.6 % after 28 days)
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
One GLP study on biodegradation (OECD 301F) was conducted with non-adapted activated sludge. The manometric respirometry test resulted in 1.6 % degradation of the substance after 28 days. Hence, the substance is not readily biodegradable under the test conditions. Further, the test item did not inhibit the activity of activated sludge microorganisms at 41.8 mg/L (biodegradation in toxicity control was ≥ 25 % after 14 days of incubation). The biodegradation of the reference substance sodium benzoate (56.9 mg/L) amounted to 87.7 % after 14 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.