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EC number: 940-417-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-06-06 to 2012-06-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study, no deficiencies
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 5,8,11,14,17-pentaoxahenicosane; 5,8,11,14-tetraoxaoctadecane
- EC Number:
- 940-417-3
- Molecular formula:
- C14H30O4 C16H34O5
- IUPAC Name:
- 5,8,11,14,17-pentaoxahenicosane; 5,8,11,14-tetraoxaoctadecane
- Details on test material:
- - Name of test material (as cited in study report): Polyglycol BB 300
- Physical state: Liquid, colourless
- Analytical purity: 99.6 % (a/a)
- Purity test date: 2011-06-28
- Lot/batch No.: DEG4155254
- Expiration date of the lot/batch: 2013-06-24
- Stability under test conditions: Not applicable
- Storage condition of test material: Room temperature, protected from light, in original container.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 12.5 – 25.0 – 50.0 – 100 – 200 mg/L
- Sampling method: The test item Polyglycol BB 300 was analytically verified by GC-MS in the fresh media (0 h) and old media (48 h) of all concentration levels and the control.
Sampling from the freshly prepared media was carried out immediately after homogenisation. Samples of the old media were taken from additional replicates, prepared with test media, but without daphnids. These replicates were incubated under test conditions until sampling. Recoveries of the test item should be within ± 20% of the nominal concentrations.
- Sample storage conditions before analysis:All original samples were stored at 6 ± 2 °C until sample preparation, if necessary. Prepared samples were stored at room temperature until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution (200 mg/L test item was weighed out) was freshly prepared with dilution water and agitated.
- Eluate: Dilution water
- Differential loading: 12.5 – 25.0 – 50.0 – 100 – 200 mg/L
- Controls: Dilution water without test item tested under the same conditions as the test groups.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- Lufthygiene (WaBoLu), further own breeding
- Age at study initiation (mean and range, SD): 2 to 24 h
- Method of breeding: In 2-3 L glass vessels with appr. 1.8 L culture medium, at 20 +/- 2 °C, in an incubator, 16 h illumination, illumination strength max. 20 µExm-2 x s-1
- Feeding during test: No feeding
ACCLIMATION
- Acclimation period: 2 h
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: No feeding
- Health during acclimation (any mortality observed): Healthy, no mortality observed
DILUTION WATER:
Dilution water ISO test water, according to OECD 202, Annex 3:
Component Concentration [mg/L]
KCl 5.76
NaHCO3 64.8
CaCl2 2 H2O 294
MgSO4 7 H2O 123
pH-value: 7.8 0.2
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
Test conditions
- Hardness:
- Dilution water at test start: 253 (mg CaCO3/L)
- Test temperature:
- 20.8 °C (Water)
- pH:
- Water Quality Parameters at 0 h (Fresh Media)
(measured in one additional replicate per concentration level and control)
Polyglycol BB 300
Nominal
Test Item Concentration
[mg/L] pH-Value
200 7.80
100 7.66
50.0 7.75
25.0 7.77
12.5 7.73
Control 7.81
Water Quality Parameters after 48 h (Old Media)
(measured in all replicates)
Polyglycol BB 300
Nominal
Test Item Concentration
[mg/L] pH-Values
Replicates
1 2 3 4
200 7.70 7.70 7.69 7.69
100 7.66 7.67 7.69 7.69
50.0 7.69 7.68 7.67 7.66
25.0 7.72 7.71 7.70 7.69
12.5 7.67 7.69 7.69 7.70
Control 7.66 7.64 7.64 7.65 - Dissolved oxygen:
- Water Quality Parameters at 0 h (Fresh Media)
(measured in one additional replicate per concentration level and control)
Polyglycol BB 300
Nominal
Test Item Concentration
[mg/L] Dissolved O2-Concentration
[mg/L]
200 8.76
100 8.77
50.0 8.73
25.0 8.74
12.5 8.71
Control 8.89
Water Quality Parameters after 48 h (Old Media)
(measured in all replicates)
Polyglycol BB 300
Nominal
Test Item Concentration
[mg/L] Dissolved O2 -Concentration [mg/L]
Replicates
1 2 3 4
200 8.33 8.41 8.39 8.44
100 8.17 8.29 8.31 8.23
50.0 8.17 8.10 8.18 8.10
25.0 8.30 8.19 8.12 8.10
12.5 8.27 8.26 8.29 8.30
Control 8.26 8.16 8.22 8.35 - Salinity:
- Not measured, freshwater
- Nominal and measured concentrations:
- please refer to "Any other information on materials and methods"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers, 50 mL
- Type (delete if not applicable): closed with parafilm
- Material, size, headspace, fill volume: Glass beakers (4 cm ID x 7 cm H), 50 mL
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): None
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: see above
- Conductivity: Dilution water at test start:654 µS/cm
- Culture medium different from test medium: Elendt M4 acc. to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
- Intervals of water quality measurement: At test start and test end
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16:8 light:dark cycle
- Light intensity: Diffuse light, illumination strength max. 20 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobility was determined in all groups after 24 h and 48 h.
TEST CONCENTRATIONS
- Range finding study:
Immobilisation Rates in the non GLP Preliminary Test
(n = 20, divided into 2 replicates with 10 daphnids each)
Nominal
Test Item
Concentration
[mg/L] IMMOBILISATION [%]
24 h 48 h
Replicates Replicates
1 2 MV 1 2 MV
300 80 80 80 100 100 100
100 40 20 30 100 100 100
30 10 10 10 0 0 0
10 0 0 0 0 0 0
Control 0 0 0 0 0 0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 65.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI 58.6-73.8 mg/L
- Details on results:
- - Mortality of control: 0 %
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50/LC50: 1.76 (CI 1.50 - 2.01) mg/L - Reported statistics and error estimates:
- EC10- and the EC50-values were calculated by sigmoidal dose-
response regression. The confidence intervals were calculated from the best-fit values, the standard error and the t-distribution with the software GraphPad Prism5, wherever possible. The concentration effect relationships are shown graphically.
Any other information on results incl. tables
Immobilisation Rates in the Definitive Test after 24 and 48 h of Exposure
(n = 20, divided into 4 replicates with 5 daphnids each)
Polyglycol BB 300 |
IMMOBILISATION [%] |
|||||||||
Nominal Test Item Concentration [mg/L] |
24 h |
48 h |
||||||||
Replicates |
Replicates |
|||||||||
1. |
2. |
3. |
4. |
MV |
1. |
2. |
3. |
4. |
MV |
|
200 |
80 |
60 |
60 |
60 |
65 |
100 |
100 |
100 |
100 |
100 |
100 |
40 |
40 |
20 |
60 |
40 |
80 |
80 |
60 |
100 |
80 |
50.0 |
0 |
0 |
0 |
0 |
0 |
40 |
20 |
40 |
20 |
30 |
25.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
12.5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the nominal concentrations of the test item Polyglycol BB 300 the 48-h EC50 for Daphnia magna was estimated to be 65.4 mg/L (95 % confidence limits: 58.6 – 73.8 mg/L). The NOEC after 48 h was 25.0 mg/L. The LOEC after 48 h was 50.0 mg/L.
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item Polyglycol BB 300 (batch number: DEG4155254) were determined according to OECD 202 (2004) from 2012-06-06 to 2012-06-11, with the definitive exposure phase from 2012-06-06 to 2012-06-08, at Dr.U.Noack-Laboratorien, Käthe-Paulus-Str.1, 31157 Sarstedt, Germany .
The study was conducted in sealed glass flasks under static conditions over a period of 48 h with 5concentration levels of the test item Polyglycol BB 300 in the range of 12.5 to 200 mg/L prepared with dilution water in a geometric series with a dilution factor of 2. Twenty daphnids were exposed to each concentration level and the control.
The concentrations of the test item Polyglycol BB 300 were analytically verified by GC-MS in the fresh media (0 h) and in the old media (48 h) of all concentration levels and the control. The measured concentrations of the test item Polyglycol BB 300 in the fresh media (0 h) were in the range of 91 to 105 % of the nominal values. The measured concentrations of the test item in the old media (48 h) were in the range of 91 to 119 % of the nominal values, indicating that the concentrations of the test item Polyglycol BB 300 were successfully maintained for the duration of the test.
The endpoints were assessed based on the nominal concentrations of the test item Polyglycol BB 300 (Table 1).
The water quality parameters (pH-value and dissolved oxygen concentration), measured at 0 and 48 h, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.
Table 1: EC10-, EC50- (with Confidence Interval), EC100-Values, NOEC and LOEC
(based on the nominal concentrations of the test item Polyglycol BB 300)
Endpoints
(with 95 % confidence limits)
Test
Duration
[h]
Polyglycol BB 300
Nominal Concentration
of the Test Item
[mg/L]
EC10
24
54.4 (CI: 43.5 – 67.6)
48
34.5 (CI: 27.0 – 42.1)
EC50
24
132 (CI: 111 - 156)
48
65.4 (CI: 58.6 – 73.8)
EC100
24
> 200
48
200
NOEC
48
25.0
LOEC
48
50.0
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