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EC number: 940-332-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-02-05 to 2014-03-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Biphenyl-4-yl-(9,9-dimethyl-9H-fluoren-2-yl)-(9,9‘-spirobifluoren-4-yl)-amine
- EC Number:
- 940-332-1
- Molecular formula:
- C52H37N
- IUPAC Name:
- Biphenyl-4-yl-(9,9-dimethyl-9H-fluoren-2-yl)-(9,9‘-spirobifluoren-4-yl)-amine
Constituent 1
- Specific details on test material used for the study:
- Designation: Art. 139301
Synonym: HTM-163
Appearance: pale yellow powder without visible impurities
Released until: August 15, 2016
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, Rossdorf, Germany.
- Pretreatment:
The aerobic activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and centrifuged again. This procedure was done three times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material per litre were mixed with test water and aerated for two days. This suspension was used for the experiment.
- Concentration of sludge: 28.7 mg/L
- Water: deionised water - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Initial conc.:
- 286.8 mg/L
- Based on:
- ThOD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Analytical grade salts were added to deionised water to prepare the following stock solutions:
a) 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4 x 2 H2O, 0.5 g NH4Cl filled up with deionised water to 1000 mL volume; The pH-value was 7.4.
b) 22.5 g MgSO4 x 7 H2O filled up with deionised water to 1000 mL volume
c) 36.4 g CaCl2 x 2 H2O filled up with deionised water to 1000 mL volume
d) 0.25 g FeCl3 x 6 H2O filled up with deionised water to 1000 mL volume In order to avoid precipitation of iron hydroxide in the stock solution
d) after storage and before use, one drop of concentrated HCl per litre was added.
50 mL of stock solution a) and 5 mL of the stock solutions b) to d) were combined and filled up to a final volume of 5000 mL with deionised water.
- Additional substrate: -
- Solubilising agent (type and concentration if used): -
- Test temperature: 22°C ± 1°C,
- pH: 7.5
- pH adjusted: no
- Suspended solids concentration: 28.7 mg/L
- Continuous darkness: yes
- Other:
TEST SYSTEM
- Culturing apparatus: Manometric Test System with test flasks containing a volume of approximately 500 mL.
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Manometric Test System
- Method used to create anaerobic conditions: Manometric Test System
- Measuring equipment: Manometric Test System
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used: Potassium hydroxide solution (45%)
- Other:
SAMPLING
- Sampling frequency: daily
- Sampling method: Manometric Test System
- Sterility check if applicable: -
- Sample storage before analysis: -
- Other: -
CONTROL AND BLANK SYSTEM
- Inoculum blank:
- Abiotic sterile control:
- Toxicity control:
- Other:
STATISTICAL METHODS:
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- Results of the Functional Control
The reference item sodium benzoate was sufficiently degraded to 82% after 14 days and to 85% after 28 days of incubation.
Results of the Toxicity Control
In the toxicity control containing both, the test item and reference item, 29% biodegradation was noted within 14 days
and 31% biodegradation was determined after 28 days of incubation.
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- no biodegradation was observed
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test item is classified as not readily biodegradable in the 10-d-window and after 28 days.
- Executive summary:
The test item is classified as not readily biodegradable in the 10-d-window and after 28 days.
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