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EC number: 684-597-9 | CAS number: 1072005-10-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Oxygen-consumption test according to Hüls AG (Dr. Schöberl)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- The test item was emusified in Nonylphenol plus 10 E0 plus 5 PO.
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Test organisms: Pseudomonas putida Migula
- Laboratory culture: from Dr. Reinhard Kanne, Bayer AG, Leverkusen
- Pretreatment: A liquid culture of the test organisms was incubated for 19h, 10 x 10^5 germ/mL, extinction (436 nm): 0.29 - 0.31. 80 mL of this liquid culture was inoculated with 200 mL sterile stocksolution 1 and 2 and 5 minutes aerated and mixed. Extinction of the bacteria suspension: 0.304.
Details on stocksolution 1: 20 g D(+)Glucose, 4.24 g NaNO3 p. a., 2.40 g K2HPO4 (without water), 1.20 g KH2PO4 p. a., 30 mL trace element solution (see below), 1000 mL double destilled water. Sterilise at 100 °C, 40 min with drifty steam.
Trace element solution (all vaues in g per 500 mL double destilled water): 0.0275 Al2 (SO4)3 x 18 H2O, 0.014 KJ, p. a., 0.014 KBr, p. a., 0.0275 TiO2, (Titan IV oxid), 0.014 SnCl2 x 2 H2O, p. a., 0.014 LiCl, p.a., 0.1945 MnCl2 x 4 H2O, p. a., 0.307 H3BO3, 0.0275 ZnSO4 x 7 H2O, p. a., 0.0275 CuSO6 x 5 H2O, p. a., 0.0295 NiSO4 x 6 H2O, 0.0275 Co(NO3)2 x 6 H2O, reinst
Details on stocksolution 2: 0.200 g FeSO4 x 7 H2O p.a., 3.71 g MgSO4 x 6 H2O p.a. fill up to 1L with double destilled water, shake, filter sterile - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 4.75 h
- Test temperature:
- 25.0 - 25.7 °C
- Nominal and measured concentrations:
- Nominal concentrations: 0.99, 9.9, 99, 990 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: BSB-flask
- Type: closed
- Material, fill volume: glass, 100 mL
- Aeration: no
- No. of vessels per concentration (replicates): 4 (2 for measurements at start, 2 for measurements at end)
- No. of vessels per control (replicates): 5
- No. of vessels per abiotic control (replicates): 4 (2 for measurements at start, 2 for measurements at end)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no details available
EFFECT PARAMETERS MEASURED
oxygen concentration was measured at the start and at the end of the test - Reference substance (positive control):
- no
- Duration:
- 4.75 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 990 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 4.75 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 990 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The test item caused a higher consumption of O2 compared to the control. In the highest concentration of 0.99 g/L the consuption was 13.5% higher. For details please see Table 1 below. Therefore the test item does not inhibit but stimulate the respriation.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the respiration inhibition test the EC10 was >0.99 g/L.
- Executive summary:
In the oxygen-consumption test according to Hüls AG (Dr. Schöberl) the respiration inhibition of the test substance was determined. Four concentrations of 0.99, 9.9, 99 and 990 mg/L were used. After incubation of 4.75 h with Pseudomonas putida Migula the respiration of the organisms was stimulated and not inhibited. Therefor the EC10 and EC50 > 0.99 g/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- EG-Richtlinie-Nr.: 88/302/EG (30.5.1988)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test item was pipetted directly in the test vessels.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
mainly domestic wastwater treatment plant "Marl-West"
- Pretreatment: On the day of sampling the sewage was settled twice and washed with 1-2 L drinking water. Afterwards 200 mL were filled up to 4 L with drinking water and stored aerated and stirred. On the start of the experiment it was washed twice with drinking water. The final activated sludge had a dry content of 3.79 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- 8.1 - 8.5
- Nominal and measured concentrations:
- Nominal concentrations: 45.8, 91.6, 183.2, 366.4 and 1099 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: vessels
- Type: open
- Material, fill volume: glass, 500 mL
- Aeration: yes (0.5 - 1 L/min)
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 2
- No. of vessels per reference (replicates): 3
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no details available
EFFECT PARAMETERS MEASURED
oxygen concentration was measured at the start and at the end of the test - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The test item caused a higher consumption of O2 compared to the control. In the highest concentration of 1.1 g/L the consuption was 17.9% higher. For details please see Table 1 below. Therefore the test item does not inhibit but stimulate the respriation.
- Results with reference substance (positive control):
- Reference substance: 3,5 Dichlorophenol (5, 15, 30 mg/L)
EC50-limit: 5-30 mg/L
EC50-determined: 8.7 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- In the respiration inhibition test the EC10 was >1.1 g/L.
- Executive summary:
In the oxygen-consumption test according to OECD 209 the respiration inhibition of the test substance was determined. The concentrations of 45.8, 91.6, 183.2, 366.4 and 1099 mg/L were used. After incubation of 3 h with activated sludge the respiration of the organisms was stimulated and not inhibited. Therefor the EC10 and EC50 > 1.1 g/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to Chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 4.75 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 990 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 4.75 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 990 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to Chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
Referenceopen allclose all
Table 1: Details on results
Concentration (mg/L) |
Inhbition of respiration (%) |
0.99 |
-4.4 |
9.9 |
-3.7 |
99 |
-8.7 |
990 |
-13.5 |
Table 1: Details on results
Substance |
Concentration (mg/L) |
Inhbition of respiration (%) |
Test item |
45.8 |
-17.3 |
91.6 |
-15.3 |
|
183.4 |
-17.4 |
|
366.4 |
-18.7 |
|
1100 |
-17.9 |
|
Reference substance |
5 |
38.4 |
15 |
58.0 |
|
30 |
80.7 |
Description of key information
No data on the test item is available. With the read-across substances the following results were obtained:
CAS 68583-51-7: EC10 > 0.9 g/L, NOEC = 0.9 g/L
CAS 853947-59-8: EC10 > 1.1 g/L, NOEC = 1.1 g/L
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 900 mg/L
Additional information
No data on the test item is available. With the read-across substances the following results were obtained:
In the oxygen-consumption test according to Hüls AG (Dr. Schöberl) the respiration inhibition of the read across substance CAS 68583-51-7 was determined. Four concentrations of 0.99, 9.9, 99 and 990 mg/L were used. After incubation of 4.75 h with Pseudomonas putida Migula the respiration of the organisms was stimulated and not inhibited. Therefor the EC10 and EC50 > 0.99 g/L.
In the oxygen-consumption test according to OECD 209 the respiration inhibition of the read across substance CAS 853947-59-8 was determined. The concentrations of 45.8, 91.6, 183.2, 366.4 and 1099 mg/L were used. After incubation of 3 h with activated sludge the respiration of the organisms was stimulated and not inhibited. Therefor the EC10 and EC50 > 1.1 g/L.
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