Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 604-610-3 | CAS number: 147858-26-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- , but the study was conducted in accordance with ISO/IEC 17025.
Test material
- Reference substance name:
- Reaction mass of 2-O-α-L-Rhamnopyranosyl-α-L-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoic acid and α-L-Rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoic acid
- EC Number:
- 604-610-3
- Cas Number:
- 147858-26-2
- IUPAC Name:
- Reaction mass of 2-O-α-L-Rhamnopyranosyl-α-L-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoic acid and α-L-Rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoic acid
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Non-adapted activated sludge from the aeration tank of the ARA Werdhoelzli (Zuerich, Switzerland), a municipal biological waste water treatment plant.
- Concentration of sludge: 30 mg/l dry matter in the final mixture. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 1 002 mg/L
- Based on:
- test mat.
- Remarks:
- 70.6 mg COD/L
- Initial conc.:
- 30.1 mg/L
- Based on:
- test mat.
- Remarks:
- DOC (theoretical value calculated based on the carbon content and the test concentration)
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Aerobic mineral salts medium prepared with ultrapure water (conductivity: <1.5 μS/cm; DOC: <0.5 mg/l)
- Additional substrate: None, test or reference item as sole organic carbon sources.
- Solubilising agent (type and concentration if used): None.
- Test temperature: 22 ± 1 °C
- pH: Before the test start, the pH value was checked and if necessary adjusted to 7.2 (±0.2) with NaOH or HCl. At the end of the test, the pH was measured in all flasks.
TEST SYSTEM
- Culturing apparatus: 510 ml glass bottles (tightly closed with manometric BOD measuring devices) containing a total volume of test solution of 200 ml. The bottles were equipped with stirring rods and butyl rubber quivers which contain two pellets of sodium hydroxide each to absorb the produced CO2 from the headspace.
- Number of culture flasks/concentration: 2
- Measuring equipment: The respirometric activity was measured as pressure change. Because the formed CO2 is adsorbed, O2 consumption leads to a pressure decrease, which is recorded by a manometric BOD measuring device (OxiTop®-C head; WTW Wissenschaftlich-Technische Werkstätten GmbH & Co.
KG, DE-82362 Weilheim). Dissolved organic carbon (DOC) was determined with a Shimadzu TOC-Analyzer TOC-L CSH (Shimadzu Schweiz GmbH, Römerstr. 3, CH-4153 Reinach) using the non-purgeable organic carbon (NPOC) mode.
CONTROL AND BLANK SYSTEM
- Inoculum blank: The procedure control sodium benzoate reached 88% biodegradation after 14 days, thus confirming suitability of inoculum and test conditions
- Abiotic sterile control: Not conducted.
- Toxicity control: Not conducted.
STATISTICAL METHODS: No statistical analysis was performed.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- -
- Test performance:
- All of the following validity criteria were met:
• The difference of extremes of replicate values of the at the end of the 10-d window or at the end of the test, as appropriate, must be less than 20%
• The percentage degradation of the reference item must reach the pass level of 60% by day 14
• The O2 uptake in the blank control should normally be 20-30 mg O2/l and should not be greater than 60 mg O2/l in 28 days.
• The pH value should be in the range of 6-8.5.
At the end of the test the pH value of the blank control was 7.3 and the procedure control was 7.5.
The pH values of both test units were 7.4. All pH values were therefore in the required range at the end of the test.
The temperature was in the acceptable range during the whole incubation period.
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 80.4
- Sampling time:
- 10 d
- Remarks on result:
- other: mean of two replicates
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 96
- Sampling time:
- 28 d
- Remarks on result:
- other: mean of two replicates
- Details on results:
- The biodegradability of the test item based on O2 consumption was calculated to be 96% after 28 days as compared to the chemical oxygen demand (COD).
Biodegradation of the test item started after a lag-phase of about 1 day.
The biodegradation reached about 85% at the end of the 10-d window.
The procedure control sodium benzoate reached 88% biodegradation after 14 days, thus confirming suitability of inoculum and test conditions.
The respective DOC concentrations at the beginning and at the end of the test after 28 days were (in mg/l):
- 30.1 (theoretical value calculated based on the carbon content and the test concentration) and 1.78 for the test units (mean of two replicates)
- <0.5 and 1.05 for the blank control (mean of two replicates)
- 35.21 (theoretical value calculated based on the carbon content and the test concentration) and 1.47 for the procedure control (mean of two replicates)
The calculated total elimination based on DOC measurements reached 98% for the test item and 100% for sodium benzoate, respectively. The data confirm the high biodegradation since the total elimination based on DOC was only slightly higher as compared to the biodegradation based on BOD.
The test item reached the pass level of 60% for ready biodegradability in the Manometric Respirometry Test within the 10-d window and, therefore, can be termed as readily biodegradable.
BOD5 / COD results
- Results with reference substance:
- Degradation after 3 days > 60% (validity criterion fulfilled)
94.3 % degradation after 28 days
Any other information on results incl. tables
- Evaluation of test results (calculations): see 'Attached background material'
- Data of individual O2 consumption (for test item and procedure control): see 'Attached background material'
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- - biodegradability based on O2 consumption: 96% after 28 days;
- the pass level of 60% for ready biodegradability was reached within the 10-d window: the test item can be termed as readily biodegradable. - Executive summary:
The biodegradability of the test item 'Decanoic acid, 3-[[6-deoxy-2-O-(6-deoxy-α-L-mannopyranosyl)-α-L-mannopyranosyl]oxy]-, 1-(carboxymethyl)octyl ester, mixt. with 1-(carboxymethyl)octyl 3-[(6-deoxy-α-L-mannopyranosyl)oxy]decanoate' exposed to microorganisms derived from activated sludge of a municipal sewage treatment plant was investigated under aerobic static exposure conditions, following the test guideline OECD 301 F.
The biodegradability of the test material based on O2 consumption was calculated to be 96% after 28 days as compared to the chemical oxygen demand (COD).
Biodegradation of the test item started after a lag-phase of about 1 day.
The biodegradation reached about 85% at the end of the 10-d window.
The procedure control sodium benzoate reached 88% biodegradation after 14 days, thus confirming suitability of inoculum and test conditions.
The calculated total elimination based on DOC measurements reached 98% for the test item and 100% for sodium benzoate, respectively. The data confirm the high biodegradation since the total elimination based on DOC was only slightly higher as compared to the biodegradation based on BOD.
The test material reached the pass level of 60% for ready biodegradability in the Manometric Respirometry Test within the 10-d window and, therefore, can be termed as readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.