Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 432-070-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 2008 - June 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: no melting point/range (Decomposition at temperatures > 200 °C)
- Boiling point: no boiling point (decomposition before boiling)
- Vapour pressure: <1 * 10-12 hPa at 25°C (calculation)
- Water solubility: 125.76 g/L at 20°C
- log Pow: <-2
- pKa: = 7,00+/-0,04 - Analytical monitoring:
- no
- Details on sampling:
- No analytical monitoring
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a stock solution of an appropriate concentration in deionised water was prepared. The stock solution then was used to prepare the treatments.
First Test: Limit test, 1000 mg/L (nominal concentration)
Main Study: 5 concentrations ranging from 2000 to 125 mg/L
Controls: two replicates before and two replicates after measuring reference and test item - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- -PRE-TREATMENT:
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treamtent plant. It was collected on the same day as
the respective experiment was performed and aereated during transport. Upon arrival in the test facility, sludge was washed with tap water three
times, then centrifuged n re-suspended in tap water.
The nutrition was freshly prepared. The inocolum was taken from its source, wahsed, aerated and the dry matter was determined.
-CHEMICALS AND NUTRITIENT SOLUTION:
all chemicals used in the test were "analytical grade" or "for use in microbiology":
Peptone (8.0 g)
Meat Extract (5.5g)
Urea (1.5g)
NaCl (0.35g)
CaCl2 * 2 H20 (0.2 g)
MgSO4*7 H2o (0.1 g)
K2HPO4 (1.4g)
Deionised water (ad 500mL)
- INITIAL BIOMASS CONCENTRATION:
dry matter of sludge (mg suspended solids/litre), on February 13, 2008 : 4.3
dry matter in the test (mg suspended solids/litre): on February 13, 2008: 1.72
dry matter of sludge (mg suspended solids/litre), on February 15, 2008 : 3.8
dry matter in the test (mg suspended solids/litre): on February 15, 2008: 1.52 - Test type:
- other: aerobic
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- no data given in the test report
- Hardness:
- deionised water was used
- Test temperature:
- 20 +/- 2°C
- pH:
- 7.1 -7.6
- Dissolved oxygen:
- no data given in the test report
- Salinity:
- no data given in the test report
- Nominal and measured concentrations:
- First Test (limit test): 1000 mg/L nominal conc.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Aeration: purified air, using pasteur pipettes, flow approx. 0.75 L/min
- Feeding: nutrient solution, 16 mL/vessel - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenole (CAS 591-35-5)
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 270 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Any observations: The test item showed inhibition values which were not correlated to its concentration. At Concentrations 2000 and 1000 mg/L, approx. the same inhibition was found. It is believed that the test system, consisting of a wide variety of microorganisms, is not uniformly affected by the test item.
- Results with reference substance (positive control):
- results considered valid
- Reported statistics and error estimates:
- none
- Validity criteria fulfilled:
- yes
- Conclusions:
- The following results for the test item were determined:
3h NOEC = below 125 mg/L
3h EC20 = 270 mg/L (95% confidence interval: 110 - 430 mg/L) - Executive summary:
Two valid experiments were performed:
First, a limit-test with two replicates using the nominal concentration 1000 mg/L was performed.
The treatment 1000 mg/L showed a mean inhibition of the respiration rate of the activated sludge of 31%.
Then, a main study was performed using five concentrations ranging from 2000 to 125 mg/L.
The following inhibitions of the respiration rate of the activated sludge were detected: At 2000 mg/L 29.5 %, at 1000 mg/L 30.0 %, at 500 mg/L 24.5 %, at 250 mg/L 19.5 % and at 125 mg/L 15.8% inhibition were detected.
The test item showed inhibition values which were not correlated to its concentration. At Concentrations 2000 and 1000 mg/L, approx. the same inhibition was found. It is believed that the test system, consisting of a wide variety of micro-organisms, is not uniformly affected by the test item.
The following results for the test itemwere determined:
3h NOEC = below 125 mg/L
3h EC20 = 270 mg/L (95% confidence interval: 110 - 430 mg/L)
3h EC50 > 2000 mg/L
Reference
In both studies, the inhibition which was caused by the test item did not rise above 30 %, which were observed in the treatments 2000 and 1000 mg/L. At the lowest concentration of 125 mg/L, 15.6 % inhibition were observed.
It might be possible that the test item shows inhibitory action only to part of the microorganisms which are present in the activated sludge, so that respiration of these microorganisms is nearly completely inhibited by approx. 500 mg/L test item concentration. The micro-organisms which are not susceptible to toxic effects of the test item are not inhibited at all up to the highest concentrations tested. Therefore, no exact 3h EC50 value and no exact NOEC value could be determined. The 3h EC50 value is given as > 2000 mg/L and the NOEC is stated as 125 mg/L.
Description of key information
Not acute toxic to bacteria.
3h NOEC < 125 mg/L
3h EC20 = 270 mg/L
3h EC50 > 2000 mg/L
EC10(16 h) = 2600 mg/L
EC50(16 h) = 7500 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2 000 mg/L
Additional information
Two studies were determined.
In the key study two valid experiments were performed:
First, a limit-test with two replicates using the nominal concentration 1,000 mg/L
The treatment 1,000 mg/L showed a mean inhibition of the respiration rate of the activated sludge of 31%.
Then, a main study was performed using five concentrations ranging from 2,000 to 125 mg/L.
The following inhibitions of the respiration rate of the activated sludge were detected: At 2000 mg/L 29.5 %, at 1000 mg/L 30.0 %, at 500 mg/L 24.5 %, at 250 mg/L 19.5 % and at 125 mg/L 15.8% inhibition were detected.
The following results for the test item were determined:
3h NOEC = below 125 mg/L
3h EC20 = 270 mg/L (95% confidence interval: 110 - 430 mg/L)
3h EC50 > 2000 mg/L
In the supporting study the following values were determined:
EC10(16 h) = 2600 mg/L
EC50(16 h) = 7500 mg/L
The substance is not acute toxic for bacteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.