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EC number: 300-644-5 | CAS number: 93951-21-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- see read across justification in section 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 19 d
- pH:
- 4
- Temp.:
- 60
- Initial conc. measured:
- 209.8 mg/L
- Duration:
- 168 h
- pH:
- 4
- Temp.:
- 70
- Initial conc. measured:
- 211 mg/L
- Duration:
- 71 h
- pH:
- 4
- Temp.:
- 80
- Initial conc. measured:
- 230 mg/L
- Duration:
- 52 h
- pH:
- 7
- Temp.:
- 25
- Initial conc. measured:
- 221 mg/L
- Duration:
- 60 min
- pH:
- 9
- Temp.:
- 20
- Initial conc. measured:
- 226 mg/L
- Preliminary study:
- at 50°C after 2.4h the following results were observed: pH4: 1.7% degradation
pH7: 98.9% degradation
pH9: >99% degradation
at 50°C and pH 4 after 5 d a degradation of 10% was obserfed - Transformation products:
- yes
- No.:
- #1
- No.:
- #2
- No.:
- #2
- No.:
- #3
- Details on hydrolysis and appearance of transformation product(s):
- #1: retention time in HPLC: 11.2 minutes: after 2.4 h 75% at 50°C and pH7 (Reactive Black 5 bis-vinyl)
#2: retention time in HPLC of both isomers: 8.6 min;
#3: retention time in HPLC: 8.2 min - % Recovery:
- 33.5
- pH:
- 4
- Temp.:
- 80 °C
- Duration:
- 71.5 h
- % Recovery:
- 42.3
- pH:
- 4
- Temp.:
- 70 °C
- Duration:
- 168 h
- % Recovery:
- 48
- pH:
- 4
- Temp.:
- 60 °C
- Duration:
- 18.98 d
- % Recovery:
- 41.7
- pH:
- 7
- Temp.:
- 25 °C
- Duration:
- 51.9 h
- % Recovery:
- 45.3
- pH:
- 9
- Temp.:
- 20 °C
- Duration:
- 60 min
- pH:
- 4
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- <= 0 d-1
- DT50:
- 258.5 wk
- pH:
- 7
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- <= 0.015 h-1
- DT50:
- 1.9 d
- pH:
- 9
- Temp.:
- 20 °C
- Hydrolysis rate constant:
- <= 0.015 min-1
- DT50:
- 48 min
- Validity criteria fulfilled:
- yes
- Conclusions:
- At environmental conditions, the test substance is hydrolytically unstable at pH 7 and 9 and hydrlotically stable at pH 4
- Executive summary:
The structural analogue was tested for abiotic degradation according to OECD 111. In addition to pH 4, 7, 9 as required by the guideline, the substance was also tested at pH 11 (dyeing conditions) and pH 1.2.
At pH 1.2 and 37°C, there was no abiotic degradation within the 5 days observation period. At pH 4, 7, 9, and 11, the test substance was not stable at 50°C. The degration at pH 4, 7, and 9 where hence investiated further.
At pH 4, the half-life at 25°C is ca. 5 years; at pH 7, the half-life at 25°C is ca. 2 days; at pH 9, the half-life at 20°C is ca. 50 minutes.
pH
T [°C]
k=<
t1/2
4
25
0.0004 1/d
258.5 weeks
7
25
0.0152 1/d
1.9 days
9
20
0.0146 1/min
48 minutes
During degradation of the bis-ester, both isoforms of the mono-vinyl form appear temporarily as degradation product. One main degradation product is the bis-vinyl as the active dye-form which further hydrolyses to the hydroxy form (-OH).
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- GLP compliance:
- yes
- Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Sample storage conditions before analysis: inert gas atmosphere; at the dark; the period from dissolution up to the testing at thermostated temeprature was kept as short as possible
- Buffers:
- - pH: 4, 7, 9
- Type and final molarity of buffer:
- Composition of buffer:
pH4: potassium hydrogene phthtalate in water; adjustment of the pH by sodium hydroxide
pH7: monopotassium dihydrogene phosphate in water; adjustment of the pH by sodium hydroxide
pH9: boric acid in aqueous potassium chloride solution; adjustment of the pH with sodium hydroxide - Estimation method (if used):
- not applicable
- Details on test conditions:
- TEST SYSTEM
- Type, material and volume of test flasks, other equipment used:
- Sterilisation method:
- Lighting:
- Measures taken to avoid photolytic effects:
- Measures to exclude oxygen:
- Details on test procedure for unstable compounds:
- Details of traps for volatile, if any
- If no traps were used, is the test system closed/open
- Is there any indication of the test material adsorbing to the walls of the test apparatus?
TEST MEDIUM
- Volume used/treatment
- Kind and purity of water:
- Preparation of test medium:
- Renewal of test solution:
- Identity and concentration of co-solvent:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Dissolved oxygen: - Duration:
- 19 d
- pH:
- 4
- Temp.:
- 60
- Initial conc. measured:
- 209.8 mg/L
- Duration:
- 168 h
- pH:
- 4
- Temp.:
- 70
- Initial conc. measured:
- 211 mg/L
- Duration:
- 71 h
- pH:
- 4
- Temp.:
- 80
- Initial conc. measured:
- 230 mg/L
- Duration:
- 52 h
- pH:
- 7
- Temp.:
- 25
- Initial conc. measured:
- 221 mg/L
- Duration:
- 60 min
- pH:
- 9
- Temp.:
- 20
- Initial conc. measured:
- 226 mg/L
- Positive controls:
- no
- Negative controls:
- no
- Preliminary study:
- at 50°C after 2.4h the following results were observed: pH4: 1.7% degradation
pH7: 98.9% degradation
pH9: >99% degradation
at 50°C and pH 4 after 5 d a degradation of 10% was obserfed - Transformation products:
- yes
- No.:
- #1
- No.:
- #2
- No.:
- #2
- No.:
- #3
- Details on hydrolysis and appearance of transformation product(s):
- #1: retention time in HPLC: 11.2 minutes: after 2.4 h 75% at 50°C and pH7 (Reactive Black 5 bis-vinyl)
#2: retention time in HPLC of both isomers: 8.6 min;
#3: retention time in HPLC: 8.2 min - % Recovery:
- 33.5
- pH:
- 4
- Temp.:
- 80 °C
- Duration:
- 71.5 h
- % Recovery:
- 42.3
- pH:
- 4
- Temp.:
- 70 °C
- Duration:
- 168 h
- % Recovery:
- 48
- pH:
- 4
- Temp.:
- 60 °C
- Duration:
- 18.98 d
- % Recovery:
- 41.7
- pH:
- 7
- Temp.:
- 25 °C
- Duration:
- 51.9 h
- % Recovery:
- 45.3
- pH:
- 9
- Temp.:
- 20 °C
- Duration:
- 60 min
- pH:
- 4
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- <= 0 d-1
- DT50:
- 258.5 wk
- pH:
- 7
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- <= 0.015 h-1
- DT50:
- 1.9 d
- pH:
- 9
- Temp.:
- 20 °C
- Hydrolysis rate constant:
- <= 0.015 min-1
- DT50:
- 48 min
- Validity criteria fulfilled:
- yes
- Conclusions:
- At environmental conditions, the test substance is hydrolytically unstable at pH 7 and 9 and hydrlotically stable at pH 4
- Executive summary:
The structural analogue, Reactive Black 5, was tested for abiotic degradation according to OECD 111. In addition to pH 4, 7, 9 as required by the guideline, the substance was also tested at pH 11 (dyeing conditions) and pH 1.2.
At pH 1.2 and 37°C, there was no abiotic degradation within the 5 days observation period. At pH 4, 7, 9, and 11, the test substance was not stable at 50°C. The degration at pH 4, 7, and 9 where hence investiated further.
At pH 4, the half-life at 25°C is ca. 5 years; at pH 7, the half-life at 25°C is ca. 2 days; at pH 9, the half-life at 20°C is ca. 50 minutes.
pH
T [°C]
k=<
t1/2
4
25
0.0004 1/d
258.5 weeks
7
25
0.0152 1/d
1.9 days
9
20
0.0146 1/min
48 minutes
During degradation of the bis-ester, both isoforms of the mono-vinyl form appear temporarily as degradation product. One main degradation product is Reactive Black 5 bis-vinyl as the active dye-form which further hydrolyses to Reactive Black 5-OH.
Referenceopen allclose all
Description of key information
Substance is not hydrolytically stable at pH 7, 9, and 11 at environmental or use-conditions
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 2 d
- at the temperature of:
- 25 °C
Additional information
The structural analogue, Reactive Black 5, was tested for abiotic degradation according to OECD 111. In addition to pH 4, 7, 9 as required by the guideline, the substance was also tested at pH 11 (dyeing conditions) and pH 1.2.
At pH 1.2 and 37°C, there was no abiotic degradation within the 5 days observation period. At pH 4, 7, 9, and 11, the test substance was not stable at 50°C. The degradation at pH 4, 7, and 9 were hence investigated further.
At pH 4, the half-life at 25°C is ca. 5 years; at pH 7, the half-life at 25°C is ca. 2 days; at pH 9, the half-life at 20°C is ca. 50 minutes.
During degradation of the bis-ester, both isoforms of the mono-vinyl form appear temporarily as degradation product. One main degradation product is Reactive Black 5 bis-vinyl as the active dye-form which further hydrolyses to Reactive Black 5-OH.
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