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EC number: 287-024-7 | CAS number: 85408-62-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-07-18 to 2008-03-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study conducted according to GLP and OECD (202) (Guidelines for Testing of Chemicals. Section 2. Guideline 202 "Daphnia sp. Acute Immobilisation Test" (2004)). The analytical chemistry supporting this study was performed to GLP and confirms the identity of the test item but, the separate chemistry report has not been audited at the current time (04 November 2010).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- Name of test material as cited in test report: Sodium Cocoyl Isethionate Common name SLI (76) Stripped.
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Nominal Concentrations: 0, 18, 32, 56, 100, 180 mg/L
- Sampling method: Test item concetrations (Nominal 18, 32, 56, 100 and 180 mg/L) were determined at 0, 24 and 48 hrs. The stock solution and test media were removed and renewed after 24 hours and concentrations determined using LC/MS.
- Sample storage conditions before analysis: No data provided.
Test solutions
- Details on test solutions:
- A nominal 1800 mg/L stock solution was prepared by adding 1800 mg of SLI (76) stripped to 1000 mL of purified water. The solution was heated to approximately 40-50 degrees C whilst stirring continuously until test item dissolved. Test media were prepared by diluting the warm 1800 mg/L stock solution with Elendt M7 medium to obtain nominal test concentrations of 0, 18, 32, 56, 100 and 180 mg/L. The test solutions were left to stand for 1 hour after preparation to allow for settling of the test item precipitate. The resulting supernatant was decanted and used as the final test media.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation (mean and range, SD):<24 hrs old
- Daphnia were not fed duing the test
ACCLIMATION
- Acclimation period: <24 h
- Acclimation conditions (same as test or not): no data
- Type and amount of food: no food
- Feeding frequency: not applicable
- Health during acclimation (any mortality observed): no data
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- Measured in 0 and 180 mg/L nominal test solutions at 0, 24 and 48 hrs. Mean = 276 (SD = 10) mg/L as CaCO3
- Test temperature:
- The temperature was measured in five random test vessels at 0, 24 and 48 hrs. Mean = 20.0 (SD = 0.6) degrees C
- pH:
- The pH was measured in all test concentrations at 0, 24 and 48 hrs. Mean = 7.6 (SD = 0.2)
- Dissolved oxygen:
- The dissolved oxygen concetration was measured in all test concentrations at 0, 24 and 48 hrs. Mean = 8.0 (SD = 1.2) mg/L
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal: 0, 18, 32, 56, 100 and 180 mg/L
Measured concentrations reported as time weighted averages for both total and dissolved:
Total measured concentrations: 15.7, 29.1, 49.8, 101.6 and 169.2 mg/L
Dissolved measured concentrations: 10.8, 19.6, 34.6, 50.1 and 80.6 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 120 mL crytallising dish
- Renewal rate of test solution (frequency/flow rate): every 24 hours
- No. of organisms per vessel: five
- No. of vessels per concentration (replicates): four replicates per concentration
- No. of vessels per control (replicates): four
- No. of vessels per vehicle control (replicates): not applicable
- Biomass loading rate: no data
TEST MEDIUM / WATER PARAMETERS
Elendt M7 media. No details provided.
OTHER TEST CONDITIONS
Normal laboratory light intensity with photoperiod of 16 h light and 8 h dark.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobility in the Daphnia is due mainly to indirect effects. At 48 hours the Daphnia exposed to 56 mg/L (nominal) SLI(76) stripped were fully mobile however there was evidence of the precipitate adhering to the carapace and antennae. Similarly at 100 mg/L (nominal), the majority of the Daphnia were coated in precipitate around the head, carapace and antennae although they were still considered mobile. At 180 mg/L (nominal), individuals were severely hindered by test precipitate and were considered immobile according to the criteria set by OECD i.e. unable to swim after 15 seconds agitation. It should be noted however that no mortality was observed. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 101.6 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% Confidence Interval
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 50 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence interval
- Details on results:
- None of the concetrations caused 100% immobility.
Immobility in the Daphnia is due mainly to indirect effects. At 48 hours the Daphnia exposed to 56 mg/L (nominal) SLI(76) stripped were fully mobile however there was evidence of the precipitate adhering to the carapace and antennae. Similarly at 100 mg/L (nominal), the majority of the Daphnia were coated in precipitate around the head, carapace and antennae although they were still considered mobile. At 180 mg/L (nominal), individuals were severely hindered by test precipitate and were considered immobile according to the criteria set by OECD i.e. unable to swim after 15 seconds agitation. It should be noted however that no mortality was observed and it is believed that without the precipitation no effects would have been observed. - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- 95% confidence intervals
Any other information on results incl. tables
TABLE 1. SUMMARY OF SLI (76) STRIPPED CONCENTRATIONS during 48 hour Daphnia magna study
Nominal concentration (mg/L) |
Measured concentration (mg/L) |
Mean measured concentration (mg/L)* |
||||||||
0 hours |
24 hrs (old) |
24 hrs (new) |
48 hrs |
|||||||
Total |
Dissolved |
Total |
Dissolved |
Total |
Dissolved |
Total |
Dissolved |
Total |
Dissolved |
|
18 |
17.7 |
10.1 |
17.4 |
15.4 |
18.7 |
10.3 |
10.0 |
7.9 |
15.7 |
10.8 |
32 |
30.3 |
15.4 |
31.4 |
27.6 |
31.6 |
17.3 |
23.5 |
19.4 |
29.1 |
19.6 |
56 |
53.4 |
39.4 |
50.1 |
37.4 |
54.3 |
41.0 |
42.1 |
22.4 |
49.8 |
34.6 |
100 |
102.8 |
54.4 |
93.9 |
40.7 |
103.8 |
67.8 |
106.1 |
40.4 |
101.6 |
50.1 |
180 |
169.6 |
100.6 |
155.9 |
61.1 |
168.6 |
116.9 |
183.2 |
54.8 |
169.2 |
80.6 |
* calculated as time weighted means
Table 2. 24 & 48 hour immobility data
Nominal |
% immobility |
|
Conc (mg/L) |
at 24 hrs |
at 48 hrs |
0.0 |
0 |
0 |
18 |
0 |
0 |
32 |
0 |
0 |
56 |
0 |
0 |
100 |
0 |
0 |
180 |
10 |
60 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 of SLI(76) stripped to juvenile (<24 h old) Daphnia magna based on total mean measured concentrations was > 101.6 (95 % confidence intervals). The 48-hour EC50 based on dissolved mean measured concentrations was >50.0 (95 % confidence intervals).
- Executive summary:
The 48-hour EC50 of SLI(76) stripped to juvenile (<24 h old) Daphnia magna, under semi-static conditions, based on total mean measured concentrations was > 101.6 (95 % confidence intervals). The 48-hour EC50 based on dissolved mean measured concentrations was >50.0 (95 % confidence intervals). Test concentrations were determined throughout the study by LC/MS. The study was conducted according to GLP and OECD (202) testing guideline.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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