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EC number: 275-864-7 | CAS number: 71701-15-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Hydrogen bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-), compound with 2-ethylhexylamine (1:1)
- EC Number:
- 275-864-7
- EC Name:
- Hydrogen bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-), compound with 2-ethylhexylamine (1:1)
- Cas Number:
- 71701-15-0
- Molecular formula:
- C42H44CrN9O6
- IUPAC Name:
- Chromate(1-), bis[2-[2-[4,5-dihydro-3-methyl-5-(oxo-kO)-1-phenyl-1H-pyrazol-4-yl]diazenyl-kN1]benzoato(2-)-kO]-, hydrogen, compd. with 2-ethyl-1-hexanamine (1:1:1)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Preparation of the saturated solution:
The saturated solution (1.00 mg/L test item were weighed out) was prepared with dilution water two days prior to the start of the exposure
(at -48 hours).
Treatment:
The test item was dispersed in the dilution water by stirring with approximately 1100 rpm for 48 hours at room temperature. After completion of
stirring, the test item dispersion was filtered with a membrane filter (0.45 µm, RC, MACHEREY-NAGEL) to achieve the saturated solution.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Clone 5
- Source:
Origin: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
Breeder: DR.U.NOACK-LABORATORIEN, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany
- Age at study initiation (mean and range, SD): 2 to 24 h old daphnids from a healthy stock were used for the study
- Method of breeding:
Culture: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 +- 2°C, in an incubator, 16 h illumination; light intensity
of max. 20 µE x m-2 x s-1
Culture medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
Cultur feeding: At least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and
Desmodesmus subspicatus, with an algae cell density of > 106 cells/mL
- Feeding during test: No feeding
ACCLIMATION
- Acclimation period: At least 2 h in dilution water.
- Acclimation conditions (same as test or not): Same as test conditions.
- Health during acclimation (any mortality observed): No mortality observed.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 253 mgCaCO3/L
- Test temperature:
- 18 - 22°C, constant within ± 1°C
At start of exposure: 19.7 °C - pH:
- pH at the Start of the Exposure (0 hours): 7.84
at the end of the test:
Saturated solution: 7.72 - 7.83
Control: 7.78- 7.81
Nominal loading
of the test item
[mg/L] pH-value
1.00 6.91
Control 7.65
pH at the End of the Exposure (48 hours)
(measured in all replicates)
Nominal loading
of the test item
[mg/L] pH-values
Replicates
1 2 3 4
1.00 7.26 7.26 7.27 7.31
Control 7.13 7.14 7.19 7.22 - Dissolved oxygen:
- O2-concentration at the Start of the Exposure (0 hours): 8.20 mg O2/L
at the end of the test:
Saturated solution: 8.26 mg O2/L
control: 8.04 mg O2/L
Nominal loading level
of the test item
[mg/L]
Dissolved
O2-concentration
[mg/L]
1.00 4.38
Control 8.59
O2-concentration at the End of the Exposure (48 hours)
(measured in all replicates)
Nominaltest loading level
of the test item
[mg/L] Dissolved O2 -concentration [mg/L]
Replicates
1 2 3 4
1.00 8.41 8.38 8.42 8.44
Control 8.26 8.26 8.29 8.27 - Nominal and measured concentrations:
- nominal: 100 mg/L as a saturated solution (The maximum dissolved concentration of the test item that can be achieved under the test conditions in the test medium, acc. to OECD Series, No. 23 (2000)6)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (5 cm ID x 8 cm H), 50 mL volume
- Test volume: 20 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- dilution water: According to EC 92/69 L383A C.2. Annex
- Culture medium different from test medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
OTHER TEST CONDITIONS
- Photoperiod: 16/8 h light/dark cycle
- Light intensity: Diffuse light, illumination range max. 20 μmol photons⋅m-2 ⋅ s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Immobilisation, 24 h interval
TEST CONCENTRATIONS
-The effects of the saturated solution (100 mg/L) of the test item were tested in a limit test.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- other: 5 % Immobilisation
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilisation
- Details on results:
- See below
- Results with reference substance (positive control):
- The percentage immobility for the reference item was determined after 24 h. The EC100-value was determined directly from the test results.
The EC10- and EC50- with 95 % confidence interval
(CI) was determined in a probability network by interpolation according to standard procedures.
EC-values after 24 h of the reference item in mg/L:
EC10 : 0.97
EC50 : 2.07 (CI 1.68 - 2.56)
EC100 : 5.80
The EC50-value of the reference item potassium dichromate after 24 h is within the prescribed concentration range of 1.0 - 2.5 mg/L of quality
criteria according to AQS: DIN Guideline 38412 L 30.
Any other information on results incl. tables
Biological Data
The percentage immobility was determined in the tested limit concentration and the control after 24 h and 48 h. The immobilisation rates in % after 24 h and 48 h of exposure are given in the table below. There was no biologically significant effect neither in the tested limit concentration nor
in the control group.
Percentage Number of Daphnia Incapable of Swimming after 24 and 48 hours of Exposure
Test item Nominal loading of the test item [mg/L] |
IMMOBILISATION [%] |
|||||||||
24 hours |
48 hours |
|||||||||
Replicates |
Replicates |
|||||||||
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
|
100 (Saturated solution) |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
20 |
5 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
According to OECD Guidance Document No 23 (2000) if an effect cannot be detected in a test with a saturated solution the result has to be reported as no toxic effects at saturation. Exposures are expressed in terms of the original concentration of the test item in water at the start of the mixing period (loading rate).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- At the saturated solution with a nominal loading of 100 mg/L of the test item , no biologically significant effect was determined to Daphnia magna.
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS) the effect of the saturated solution (100 mg/L) as limit concentration of the test item was determined according to OECD 202 (1999, Draft) and EC Directive 92/69/EC Method C.2 (1992) .
(Saturated solution: The maximum dissolved concentration of the test item that can be achieved under the test conditions in the test medium, acc. to OECD Series, No. 23 (2000)6).
The limit test was conducted under static conditions over 48 h.
20 test organisms were exposed to the limit concentration and control.
A reference test was carried out with potassium dichromate to determine the toxicity of the reference item. The EC50-value of the
reference item of 2.07 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS, DIN Guideline 38412 L 30. Water quality parameters pH-value and dissolved oxygen concentration, measured at 0 and 48 h, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.
At the saturated solution with a nominal loading of 100 mg/L of the test item , no biologically significant effect was determined to Daphnia magna.
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