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EC number: 248-460-3 | CAS number: 27441-70-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September 18, 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted on 4 April 1984
- Principles of method if other than guideline:
- The study described in this report was conducted in accordance with ISO/IEC 17025.
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Details on test solutions:
- These test concentrations were prepared by direct addition of the test substance.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Inoculum: activated sludge from the aeration tank of a municipal biological waste water treatment plant, not adapted, not pre-conditioned.
- Source: ARA Werdhölzli, CH-8048Zürich (18 September 2002;9.30 a.m.).
- Concentration: 1.6 g/l dry matter in the final mixture. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Nominal and measured concentrations:
- 125, 250, 500, 1000 and 2000 mg/l nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 ml flasks containing a total volume of test suspension of 500 ml.
- Aeration: aerated by shaking (120 r.p.m.).
- No. of vessels per concentration: one replicate.
- No. of vessels per control: 2 replicates.
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: synthetic sewage feed prepared with tap water.16 ml of 100-fold sewage concentrate per 500 ml of final test mixture.
EFFECT PARAMETERS MEASURED
The oxygen concentration, in mg/|, was measured with a oxygen electrode inserted to a 300 ml BOD bottle and recorded for about 5 to 10 min. - Reference substance (positive control):
- yes
- Remarks:
- 3,S-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- At the applied test concentrations, the following toxic effects to the activated sludge were observed: 2000 mg/l (45 %), 1000 mg/l (41 %), 500 mg/l (34 %), 250 mg/l (31 %),125 mg/(25 %).
Based on these data the EC20 value of test item was estimated to be lower than 125 mg/l. The EC50 and EC80 values were estimated to be higher than 2000 mg/l.
The variation between the two controls was ± 2.8 %. - Results with reference substance (positive control):
- The EC50 value of the reference substance 3,5-dichlorophenol was estimated to be 2.5 mg/ which was low but in the range of 5 mg/l thus confirming suitability of activated sludge and test conditions.
- Validity criteria fulfilled:
- yes
- Remarks:
- The variation between the two controls was ± 2.8 % and the EC50 of reference substance was 2.5 mg/l
- Conclusions:
- IC50 (3h) > 2000 mg/l
- Executive summary:
The inhibitory effect of test item on the oxygen consumption of activated sludge microorganisms of a municipal sewage treatment plant was investigated under aerobic static conditions. The inhibitory effect may include the effect on respiration and nitrification. The exposure time of the test substance on the activated sludge was 3 hours.
The nominal concentrations of test item were 125, 250, 500, 1000 and 2000 mg/l, respectively. These test concentrations were prepared by direct addition of the test substance. No chemical analysis of test concentrations was conducted. For the calculation of the effective concentration leading to 20, 50, and 80 % respiration inhibition the nominal content of the test substance was used, assuming the test compound to be stable in water over 3 h.
At the applied test concentrations, the following toxic effects to the activated sludge were observed: 2000 mg/l (45 %), 1000 mg/l (41 %), 500 mg/l (34 %), 250 mg/l (31 %),125 mg/(25 %).
Based on these data the EC20 value of test item was estimated to be lower than 125 mg/l. The EC50 and EC80 values were estimated to be higher than 2000 mg/l.
Conclusion
IC50 (3h) > 2000 mg/l
Reference
Definitive test: activated sludge respiration rates after 3 h of exposure to the test material and calculation of the inhibition.
Nominal concentration (mg/l) | O2 consumption rates (mg/O2l/h) | Respiration inhibition (%) |
Control | 23.8 | - |
Control | 25.2 | - |
Test item 125 | 18.3 | 25.1 |
Test item 250 | 16.9 | 30.9 |
Test item 500 | 16.2 | 33.8 |
Test item 1000 | 14.5 | 40.8 |
Test item 2000 | 13.4 | 45.2 |
Reference 3 | 11.5 | 53.1 |
Reference 10 | 7.0 | 71.4 |
Reference 30 | 2.7 | 88.9 |
Description of key information
IC50 (3h) > 2000 mg/l
Key value for chemical safety assessment
Additional information
The inhibitory effect of test item on the oxygen consumption of activated sludge microorganisms of a municipal sewage treatment plant was investigated under aerobic static conditions. The nominal concentrations of test item were 125, 250, 500, 1000 and 2000 mg/l.
At the applied test concentrations, the following toxic effects to the activated sludge were observed: 2000 mg/l (45 %), 1000 mg/l (41 %), 500 mg/l (34 %), 250 mg/l (31 %),125 mg/(25 %). Based on these data the EC20 value of test item was estimated to be lower than 125 mg/l. The EC50 and EC80 values were estimated to be higher than 2000 mg/l.
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