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EC number: 242-354-0 | CAS number: 18472-51-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Source: Commercial hatchery M.B. Ruysbroek B.V. (Noordvliet 159, Maassluis)
- Length at study initiation: 2.1+/- 0.1 cm
- Weight at study initiation: 0.09 +/- 0.014 g
- Feeding during test: no - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 210 mg CaCO3/L
- Test temperature:
- 24 - 26 °C
- pH:
- 7.5 - 8.0
- Dissolved oxygen:
- > 5.9 mg O2/L
- Nominal and measured concentrations:
- 1.8, 3.2, 5.6, 10.0, 18.0 mg/L + control (nominal concentrations)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 2000 mL
- Type: open. Test substance is not volatile
- Fill volume: 1500 mL
- Aeration: Slight aeration, not specified
- Renewal rate of test solution (frequency/flow rate): Semistatic, daily renewal
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- Biomass loading rate: 150 mL/fish
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: DSWL, prepared from ground water from a locality near Linschoten (NL)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: No data
- Light intensity: No data
EFFECT PARAMETERS MEASURED:
Mortality, condition of survivors, sampled every 24 h - Reference substance (positive control):
- no
- Remarks:
- not required by Guideline
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2.08 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 3.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: No mortality was observed in the controls after 24, 48, 72 and 96 hours
- Reported statistics and error estimates:
- Calculations of LC50-values by probit analysis
- Sublethal observations / clinical signs:
Effect data:
Concentration
Mortality
(n.c.)
Number
Percentage
[mg/L]
24h
48h
72h
96h
24h
48h
72h
96h
1.8
0
0
0
0
0
0
0
0
3.2
0
0
0
0
0
0
0
0
5.6
0
0
0
0
0
0
0
0
10.0
0
0
1
1
0
0
5
5
18.0
19
20
20
20
95
100
100
100
Effect concentrations related to 20% solution:
48 h [mg/L]
95 % c.l.
96 h [mg/L]
95 % c.l.
LC0
10.0 (n)
no data1
10.0 (n)2
no data1
LC50
13,4 (n)
no data1
10.4 (n)
no data1
LC100
18.0 (n)
no data1
18.0 (n)
no data1
1No confidence intervals for the LC values could be calculated because of the steep concentration-effect curve
2One fish was dead at 10.0 mg/L, but because 10 % mortality is allowed according to OECD Guideline 203 for the control fishes this concentration is recorded as the 96 h LC0
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test results indicate that Chlorhexidine digluconate is acutely toxic towards fish. The 96h-LC50 was determined to be 2.08 mg a.s./L.
Reference
Description of key information
In a study on the acute toxicity of Chlorhexidine digluconate towards fish the LC50 was determined to be 2.08 mg a.i./L, employing the zebrafish (Brachydanio rerio) as the test organism.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 2.08 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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